Polyamines of urogenital tract men as factors of apoptosis regulation spermatozoids

Плосконос, М. В.; Евдокимов, В В

doi:10.18565/urology.2019.4.74-79

Cited by 2 publications

(4 citation statements)

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“…1 ) belong to the PA group, along with putrescine and cadaverine, which are often termed diamines ( 8 ). According to our previous results the content of Spm in human semen ranges from 138.4-21,749.9 µmol/l and Spd from 17.9-1,170.8 µmol/l ( 14 ), while the levels of PA in the blood serum (and blood plasma) do not exceed 1 µmol/l ( 26 ). Thus, the seminal plasma PA concentration greatly exceeds that of the other tissues, indicating the importance of PA for normal sperm function ( 27-29 ).…”

Section: Discussionmentioning

confidence: 64%

“…Infertility is known to be associated with a change in PA concentrations in the ejaculate, which seems to influence gamete viability ( 14 , 32 , 33 ). Excessive production of PA results in a decreased number of mature spermatozoa ( 34 ), whereas lowered PA concentrations in the seminal liquid is correlated with increased sperm apoptosis ( 35 ).…”

Section: Discussionmentioning

confidence: 99%

“…They are essential for cell growth, cell proliferation and differentiation (8,9), and have the ability to coordinate the process of cell apoptosis (9)(10)(11)(12)(13). The contents of the PAs spermine (Spm) and spermidine (Spd) in the seminal plasma are significantly higher than in any other biological fluid, which highlights the importance of these low molecular weight organic polycations for human reproductive function, especially in men (10,14). However, there is a lack of studies aiming to understand the roles of the human seminal plasma PA.…”

Section: Introductionmentioning

confidence: 99%

“…However, there is a lack of studies aiming to understand the roles of the human seminal plasma PA. It has been established that higher PAs levels, such as that of Spd and Spm, are produced by the prostate gland from putrescin and decarboxylated SAM, participate in the regulation of the pH of seminal plasma, stabilize the structure of sperm DNA, activate the motility of gametes, and are considered to be a de-capacitating factor that participates in the regulation of the sperm membrane integrity during fertilization (8,(14)(15)(16). The cell membranes are considered the main targets for MW at the molecular level (2,17).…”

Section: Introductionmentioning

confidence: 99%

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Assessing the biological effects of microwave irradiation on human semen in vitro and determining the role of seminal plasma polyamines in this process

Плосконос

Zulbalaeva²,

Kurbangalieva³

et al. 2022

Biomed Rep

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The aim of the present study was to investigate the influence of millimeter-wave electromagnetic (MW) irradiation on normal and pathological human sperm in vitro, and to evaluate a possible role of polyamines (PA) in this process. The stability of sperm membranes, the number of apoptotic gametes, and the content of seminal plasma PA in the ejaculates of fertile and subfertile men were compared before and after short-term MW electromagnetic exposure in vitro. The ejaculate samples were collected from healthy donors [n=25, age 22-38 years old (y.o.), average age 30.6±1.1 y.o. (mean ± SEM)] and from subfertile men (n=78, age 25-48 y.o., average age 34.1±0.8 y.o.) and exposed to MW radiation. The electromagnetic field had a wavelength of 7.1 mm, a frequency of 42.194 GHz and an exposure time of 20 min. The fragility of sperm membranes was evaluated by their resistance to sodium chloride solution (Milovanov test) and to acetic acid (Joel test). Acrosin activity was assayed spectrophotometrically. Apoptosis was determined by the externalization of phosphatidylserine on the outer side of the sperm membrane and propidium iodide staining. The PA levels were determined by agar gel electrophoretic fractionation. An increase in the resistance of sperm membranes, a decrease in acrosin activity, a decrease in the number of apoptotic gametes and a decrease in the seminal plasma PA concentrations were found after exposure of the native human sperm to low-intensity MW irradiation. Two types of reactions were revealed for the subfertile samples. The results revealed positive bio-effects of specific microwaves on the human semen and the participation of PA in the realization of these effects.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Assessing the biological effects of microwave irradiation on human semen in vitro and determining the role of seminal plasma polyamines in this process

Плосконос

Zulbalaeva²,

Kurbangalieva³

et al. 2022

Biomed Rep

View full text Add to dashboard Cite

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Polyamines of biological fluids of the body and the diagnostic value of their determination in clinical and laboratory researches (review of literature)

Плосконос

2021

Klin. lab. diagn.

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The review provides the analysis of the content of the main polyamines (PA) - spermine, spermidine and putrescine in the most important biological fluids of the human body (blood, urine, seminal fluid, etc.). The assessment of their diagnostic and prognostic value in clinical practice is carried out. The novelty and value of assessing of the level of PA metabolites as new diagnostic markers of various diseases has been shown. Among such diseases as cancer, stroke, renal failure, for which the search for early markers is especially relevant. This survey data can be of practical interest and taken into account in estimating the level of PA and its derivatives in clinical and laboratory reseaches. The literature search for the review was carried out using the Scopus, Web of Science, MedLine, RSCI databases.

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Polyamines of urogenital tract men as factors of apoptosis regulation spermatozoids

Cited by 2 publications

References 0 publications

Assessing the biological effects of microwave irradiation on human semen in vitro and determining the role of seminal plasma polyamines in this process

Assessing the biological effects of microwave irradiation on human semen in vitro and determining the role of seminal plasma polyamines in this process

Polyamines of biological fluids of the body and the diagnostic value of their determination in clinical and laboratory researches (review of literature)

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