Polyaniline Entrapped Water-Dispersible 3MPA-ZnSe Quantum Dots and Their Application for the Development of an Enzymatic Electrochemical Nanobiosensor for the Detection of 17β-Estradiol, an Endocrine-Disrupting Compound

Jijana, Abongile Nwabisa

doi:10.1007/s12010-022-04277-w

Cited by 12 publications

(4 citation statements)

References 79 publications

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“…Entrapment requires selecting a suitable polymer to provide the enzyme-free space for dispersion, ensuring high stability, and minimizing leaching ( Nguyen et al, 2019 ). For example, Jijana, (2023) selected Horseradish Peroxidase (HRP) as a bioreceptor to achieve the detection of E2. In this work the nanocomposite 3-mercaptopropionic acid capped zinc selenide quantum dots trapped within the polyaniline (PANI: 3MPA-ZnSeQD) are used to immobilize the HRP on the gold electrode surface by entrapment, as shown in Figure 2A .…”

Section: Various Biosensors For Detecting E2mentioning

confidence: 99%

17β-estradiol biosensors based on different bioreceptors and their applications

Wang,

Kong,

Liu

et al. 2024

Front. Bioeng. Biotechnol.

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17β-Estradiol (E2) is a critical sex steroid hormone, which has significant effects on the endocrine systems of both humans and animals. E2 is also believed to play neurotrophic and neuroprotective roles in the brain. Biosensors present a powerful tool to detect E2 because of their small, efficient, and flexible design. Furthermore, Biosensors can quickly and accurately obtain detection results with only a small sampling amount, which greatly meets the detection of the environment, food safety, medicine safety, and human body. This review focuses on previous studies of biosensors for detecting E2 and divides them into non-biometric sensors, enzyme biosensors, antibody biosensors, and aptamer biosensors according to different bioreceptors. The advantages, disadvantages, and design points of various bioreceptors for E2 detection are analyzed and summarized. Additionally, applications of different bioreceptors of E2 detection are presented and highlight the field of environmental monitoring, food and medicine safety, and disease detection in recent years. Finally, the development of E2 detection by biosensor is prospected.

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Section: Various Biosensors For Detecting E2mentioning

confidence: 99%

17β-estradiol biosensors based on different bioreceptors and their applications

Wang,

Kong,

Liu

et al. 2024

Front. Bioeng. Biotechnol.

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“…The multifunctional properties of these materials extend not only to mechanical properties but also to optical, electrical, and magnetic properties [110]. A recent study was conducted by our group, focused on nanocomposites of poly(3,4-ethylene dioxythiophene) polymeric nanoparticles and graphene quantum dots in enzyme inhibition on screen-printed electrodes (SPE) [111]. In this work, the dual detection of catechol and diclofenac was carried out.…”

Section: Application Of Enzyme-based Electrochemical Nanobiosensorsmentioning

confidence: 99%

“…The developed biosensor’s LOD was found to be 0.2 × 10 −6 M towards 17β-estradiol. The authors stated that the biosensor was capable of detecting 17β-estradiol concentrations in spiked tap water samples with acceptable recoveries [ 111 ]. In another study, quantum dots were utilized to detect cholesterol.…”

Section: Application Of Enzyme-based Electrochemical Nanobiosensorsmentioning

confidence: 99%

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Novel Approaches to Enzyme-Based Electrochemical Nanobiosensors

Kilic,

Singh,

Keles

et al. 2023

Biosensors

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Electrochemistry is a genuinely interdisciplinary science that may be used in various physical, chemical, and biological domains. Moreover, using biosensors to quantify biological or biochemical processes is critical in medical, biological, and biotechnological applications. Nowadays, there are several electrochemical biosensors for various healthcare applications, such as for the determination of glucose, lactate, catecholamines, nucleic acid, uric acid, and so on. Enzyme-based analytical techniques rely on detecting the co-substrate or, more precisely, the products of a catalyzed reaction. The glucose oxidase enzyme is generally used in enzyme-based biosensors to measure glucose in tears, blood, etc. Moreover, among all nanomaterials, carbon-based nanomaterials have generally been utilized thanks to the unique properties of carbon. The sensitivity can be up to pM levels using enzyme-based nanobiosensor, and these sensors are very selective, as all enzymes are specific for their substrates. Furthermore, enzyme-based biosensors frequently have fast reaction times, allowing for real-time monitoring and analyses. These biosensors, however, have several drawbacks. Changes in temperature, pH, and other environmental factors can influence the stability and activity of the enzymes, affecting the reliability and repeatability of the readings. Additionally, the cost of the enzymes and their immobilization onto appropriate transducer surfaces might be prohibitively expensive, impeding the large-scale commercialization and widespread use of biosensors. This review discusses the design, detection, and immobilization techniques for enzyme-based electrochemical nanobiosensors, and recent applications in enzyme-based electrochemical studies are evaluated and tabulated.

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