Polyaniline nanofibres as templates for the covalent immobilisation of biomolecules

Abstract

The attachment of antibodies onto polyaniline nanofibres using covalent chemistry was investigated for the first time. Polyaniline nanofibres were functionalised post-polymerisation to attach either amide or carboxylic acid side-groups. These templates could then be further modified to attach antibodies, specifically in this instance mouse immunoglobulin G (IgG). The resultant conjugates were characterised using a variety of techniques including infrared, UVvisible and Raman spectroscopy. Conjugates were then used to detect secondary antibodies (anti-

Biotemplate Synthesis of Polyaniline@Cellulose Nanowhiskers/Natural Rubber Nanocomposites with 3D Hierarchical Multiscale Structure and Improved Electrical Conductivity

“…The dedoped PANI showed two absorption peaks at 340 and 718 nm. The first peak at 340 nm is due to the π-π* transition of the benzenoid rings and the second broad peak at 718 nm is assigned to the n-π* transition of the quinonoid rings of PANI 38 . However, the π-π* transition and the n-π* transition bands of PANI supported on CNs blue shifted to 328 and 568 nm, respectively.…”

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“…Researchers throughout the dispensation of innovations in science and technology have exploited many avenues in which plants could be useful; plants are still useful virtually in all areas in science and technology. The role of plant materials in the eco-friendly synthesis of nanoparticles are significant due to great deal of macromolecules they constitute [16,17]. Plants are very good bioreducing agents which made them highly necessary in the green synthesis of nanoparticles/nanomaterials [15].…”

Polyaniline modified flexible conducting paper for cancer detection

“…Moreover, the presence of amine groups provides an additional benefit for covalent immobilization of biomolecules with enhanced biosensing characteristics. [20][21][22] Singh et al 23 used nanostructured polyaniline and Soni et al 24 utilized polyaniline-gold nanocomposite for the detection of sexually transmitted disease (gonorrhoeae). Further, Tiwari and Gong demonstrated chitosan-co-polyaniline (CS-co-PANI) copolymer for application to breast cancer biomarker (BRCA1) detection.…”