Polyazetidine-based immobilization of redox proteins for electron-transfer-based biosensors

Frasconi, Marco; Favero, Gabriele; Fusco, Massimo Di; Mazzei, Franco

doi:10.1016/j.bios.2008.08.017

Cited by 23 publications

(12 citation statements)

References 28 publications

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“…Therefore, an interesting diagnostic is a plot of I p vs. υ 1/2 , as shown in Figure 5(b); both the oxidation peak current ( I a ) and the reduction peak current ( I c ) of the PPy/PAN–MWCNT/SPCE exhibited linear responses. The electrode reaction can be reasonably deduced as being controlled by a radial diffusion process 2 , which is related to the mass transport rate of the electroactive species to the electrode surface . Moreover, the incorporation of both conductive nanofibers and a PPy layer on the electrode surface makes the PPy/PAN–MWCNT/SPCE system quasi‐reversible ( I c / I a ≠ 1)…”

Section: Resultsmentioning

confidence: 99%

Modification of disposable screen‐printed carbon electrode surfaces with conductive electrospun nanofibers for biosensor applications

Ekabutr

Chailapakul²,

Supaphol³

2013

J of Applied Polymer Sci

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In this work, we describe an alternative approach to the surface modification of screen-printed carbon electrodes (SPCEs) to fabricate a polypyrrole/polyacrylonitrile multiwall carbon nanotube-modified screen-printed carbon electrode (PPy/PAN-MWCNT/ SPCE) using a two-step process. First, PAN loaded with 5 wt % of MWCNTs was electrospun onto a carbon layer. The CNTembedded PAN electrospun nanofibers (average diameter % 200 nm) were subsequently coated with a PPy layer via vapor-phase polymerization using p-toluenesulfonate as an oxidizing agent in a vacuum system. The electrochemical behavior of both the unmodified and the modified SPCEs were compared using cyclic voltammetry (CV) with common electroactive analytes in order to optimize the electrospinning and the vapor-phase parameters. By incorporating glucose oxidase(GOX) as an enzymatic model into the modified electrode without a mediator, we conducted a calibration study which showed that glucose could be detected by an amperometer over a linear range of 0.25-6 mM with a LOD of 15.51 lM and a sensitivity of 5.41 lA/mM cm 2 . With a mediator, the glucose detection can be performed at low potential over a linear range of 0.125-7 mM with a LOD of 0.98 mM and a sensitivity of 14.62 lA/mM cm 2 . Therefore, the novel modified electrode is a promising new device for biosensor applications. V C 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 3885-3893, 2013

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Section: Resultsmentioning

confidence: 99%

Modification of disposable screen‐printed carbon electrode surfaces with conductive electrospun nanofibers for biosensor applications

Ekabutr

Chailapakul²,

Supaphol³

2013

J of Applied Polymer Sci

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“…SAMs of thiols on gold surface seem to be the best choice for their application in biosensor construction [36]. Moreover, different immobilization procedures are available to pave the way to biorecognition element-electrode surface interaction, including covalent and noncovalent ones [37][38][39][40].…”

Section: Basic Conceptsmentioning

confidence: 99%

Biosensors for Drug Analysis

Deriu¹,

Mazzei

2012

Analytical Techniques for Clinical Chemistry

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“…In electrochemical sensors, the working electrodes are essentially modified with the active sensing materials. The physico-chemical properties of active materials affect greatly the sensor performance as well as its operational stability [5]. Therefore, the research and development for a potential active material play a decisive role in fabricating sensitive, efficient, and reliable sensing devices.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Ethanol Chemical Sensor Based on Novel Ag-Doped Mesoporous α–Fe2O3 Prepared by Modified Sol-Gel Process

et al. 2018

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Mesoporous α–Fe2O3 has been synthesized via a simple sol-gel procedure in the presence of Pluronic (F-127) triblock copolymer as structure directing agent. Silver (Ag) nanoparticles were deposited onto α–Fe2O3 matrix by the photochemical reduction approach. Morphological analysis revealed the formation of Ag nanoparticles with small sizes < 20 nm onto the mesoporous structure of α–Fe2O3 possessing < 50 nm semi-spherical shape. The XRD, FTIR, Raman, UV-vis, PL, and N2 sorption isotherm studies confirmed the high crystallinity, mesoporosity, and optical characteristics of the synthesized product. The electrochemical sensing toward liquid ethanol has been performed using the current devolved Ag/α–Fe2O3-modified glassy carbon electrode (GCE) by cyclic voltammetry (CV) and current potential (I-V) techniques, and the obtained results were compared with bare GCE or pure α–Fe2O3. Mesoporous Ag/α–Fe2O3 was found to largely enhance the sensor sensitivity and it exhibited excellent sensing characteristics during the precision detection of low concentrations of ethanol. High and reproducible sensitivity of 41.27 μAmM− 1 cm− 2 at lower ethanol concentration region (0.05 to 0.8 mM) and 2.93 μAmM− 1 cm− 2 at higher concentration zone (0.8 to 15 mM), with a limit of detection (LOD) of 15.4 μM have been achieved. Investigation on reaction kinetics revealed a characteristic behavior of mixed surface and diffusion-controlled processes. Detailed sensing studies revealed also that the sensitivity toward ethanol was higher than that of methanol or isopropanol. With further effort in developing the synthesis and fabrication approaches, a proper utility for the current proposed protocol for fabricating a better sensor device performance is possible.

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Polyazetidine-based immobilization of redox proteins for electron-transfer-based biosensors

Cited by 23 publications

References 28 publications

Modification of disposable screen‐printed carbon electrode surfaces with conductive electrospun nanofibers for biosensor applications

Modification of disposable screen‐printed carbon electrode surfaces with conductive electrospun nanofibers for biosensor applications

Biosensors for Drug Analysis

Highly Sensitive Ethanol Chemical Sensor Based on Novel Ag-Doped Mesoporous α–Fe2O3 Prepared by Modified Sol-Gel Process

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