Polycaprolactone-chitosan/multi-walled carbon nanotube: A highly strengthened electrospun nanocomposite scaffold for cartilage tissue engineering

Mirmusavi, Mohammad Hossein; Ahmadian, Mehdi; Karbasi, Saeed

doi:10.1016/j.ijbiomac.2022.04.152

Cited by 51 publications

(19 citation statements)

References 43 publications

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“…In addition, with the development of electrospun nanocomposite scaffolds containing nickel nanoparticles (Karakas et al, 2017), graphene oxide (Aidun et al, 2019), hydroxyapatite (Shirzaei Sani et al, 2021), and multi-walled carbon nanotubes (Mirmusavi et al, 2022) enhanced functional performances of complex nanostructures have been demonstrated. For example, Aidun et al (2019) investigated the biological activity and physicochemical properties of the PCL/CS scaffolds incorporated with graphene oxide.…”

Section: Introductionmentioning

confidence: 99%

“…Sani et al (Shirzaei Sani et al, 2021) incorporated hydroxyapatite nanoparticles onto PCL/CS nanofibers to enhance their mechanical properties, proliferation, cell viability, and bioactivity of them. In another work, the structural, mechanical, and biological properties of PCL/CS electrospun nanocomposite scaffolds containing multi-walled carbon nanotubes were studied by Mirmusavi et al (2022). The samples containing nanoparticles showed more cell viability and bioactivity compared to the neat samples.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication and characterization of polycaprolactone/chitosan nanofibers containing antibacterial agents of curcumin and ZnO nanoparticles for use as wound dressing

Mosallanezhad

Nazockdast

Ahmadi

et al. 2022

Front. Bioeng. Biotechnol.

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The potential of the nanoscale structure is utilized by electrospun nanofibers, which are promising materials for wound dressings. Here, we prepared wound dressings constituting polycaprolactone (PCL) and chitosan (CS). Curcumin (Cur) and zinc oxide nanoparticles (ZnO) as antibacterial agents were embedded in PCL/CS electrospun nanofibers and different properties including morphology, physicomechanical, interaction with water, antibacterial efficiency, and in vitro studies were investigated. SEM images confirmed the nanofibrous structure of samples with 100 ± 5 to 212 ± 25 nm in average diameter. Elemental analysis of nanofibers showed a good distribution of ZnO along nanofibers which not only caused decreasing in nanofiber diameter but also increased tensile strength of nanofibers up to 2.9 ± 0.5 MPa and with good elongation at break of 39 ± 2.9. ZnO nanoparticles also facilitated the interaction of nanofibers with water, and this led to the highest water vapor transition rate, which was equal to 0.28 ± 0.02 g cm−2 day−1. The sample containing 3 wt% Cur had the highest water uptake value (367 ± 15%) and the lowest water contact angle (78 ± 3.7°), although Cur has a hydrophobic nature. The release profile of Cur showed a two-stage release and the Peppas model predicted a non-fickian diffusion. Simultaneous incorporation of CS, ZnO, and Cur effectively inhibited bacterial growth. In addition, in vitro studies represented that high content of Cur decreases cell viability and cell attachment. The outcomes from the fabricated nanofibrous scaffolds demonstrated appropriate properties for application as a wound dressing.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of polycaprolactone/chitosan nanofibers containing antibacterial agents of curcumin and ZnO nanoparticles for use as wound dressing

Mosallanezhad

Nazockdast

Ahmadi

et al. 2022

Front. Bioeng. Biotechnol.

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“…This may be related to the interaction between the two polymers. This phenomenon can also increase the mechanical strength of the scaffold [ 46 ].…”

Section: Resultsmentioning

confidence: 99%

Novel Scaffold Based on Chitosan Hydrogels/Phthalated Cashew Gum for Supporting Human Dental Pulp Stem Cells

Leite

Oliveira²,

Quelemes³

et al. 2023

Pharmaceuticals

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Hydrogels are structures that have value for application in the area of tissue engineering because they mimic the extracellular matrix. Naturally obtained polysaccharides, such as chitosan (CH) and cashew gum, are materials with the ability to form polymeric networks due to their physicochemical properties. This research aimed to develop a scaffold based on chitosan and phthalated cashew tree gum and test it as a support for the growth of human mesenchymal stem cells. In this study, phthalation in cashew gum (PCG) was performed by using a solvent-free route. PCG-CH scaffold was developed by polyelectrolyte complexation, and its ability to support adherent stem cell growth was evaluated. The scaffold showed a high swelling rate. The pore sizes of the scaffold were analyzed by scanning electron microscopy. Human dental pulp stem cells (hDPSCs) were isolated, expanded, and characterized for their potential to differentiate into mesenchymal lineages and for their immunophenotypic profile. Isolated mesenchymal stem cells presented fibroblastoid morphology, plastic adhesion capacity, and differentiation in osteogenic, adipogenic, and chondrogenic lineages. Mesenchymal stem cells were cultured in scaffolds to assess cell adhesion and growth. The cells seeded on the scaffold showed typical morphology, attachment, and adequate distribution inside the matrix pores. Thus, cells seeded in the scaffold may improve the osteoinductive and osteoconductive properties of these biomaterials.

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“…Poly(ɛ-caprolactone) (PCL) is a semicrystalline polyester with melting temperatures around 55–60 °C, which makes it the perfect polymer for 3D extrusion [ 10 , 11 ]. It is the most used polymer in the TE field [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…It is the most used polymer in the TE field [ 12 ]. It is approved by the Food and Drug Administration [ 13 ], exhibits good mechanical properties, is biodegradable, has a slow degradation rate, and improves the cell differentiation and proliferation of chondrocytes [ 10 , 11 , 14 ]. However, their hydrophobicity makes cell attachment difficult [ 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Cellulose-Based Scaffolds: A Comparative Study for Potential Application in Articular Cartilage

et al. 2023

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Osteoarthritis is a highly prevalent disease worldwide that leads to cartilage loss. Tissue engineering, involving scaffolds, cells, and stimuli, has shown to be a promising strategy for its repair. Thus, this study aims to manufacture and characterise different scaffolds with poly(ε-caprolactone) (PCL) with commercial cellulose (microcrystalline (McC) and methyl cellulose (MC) or cellulose from agro-industrial residues (corncob (CcC)) and at different percentages, 1%, 2%, and 3%. PCL scaffolds were used as a control. Morphologically, the produced scaffolds presented porosities within the desired for cell incorporation (57% to 65%). When submitted to mechanical tests, the incorporation of cellulose affects the compression resistance of the majority of scaffolds. Regarding tensile strength, McC2% showed the highest values. It was proven that all manufactured scaffolds suffered degradation after 7 days of testing because of enzymatic reactions. This degradation may be due to the dissolution of PCL in the organic solvent. Biological tests revealed that PCL, CcC1%, and McC3% are the best materials to combine with human dental pulp stem/stromal cells. Overall, results suggest that cellulose incorporation in PCL scaffolds promotes cellular adhesion/proliferation. Methyl cellulose scaffolds demonstrated some advantageous compressive properties (closer to native cartilaginous tissue) to proceed to further studies for application in cartilage repair.

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Polycaprolactone-chitosan/multi-walled carbon nanotube: A highly strengthened electrospun nanocomposite scaffold for cartilage tissue engineering

Cited by 51 publications

References 43 publications

Fabrication and characterization of polycaprolactone/chitosan nanofibers containing antibacterial agents of curcumin and ZnO nanoparticles for use as wound dressing

Fabrication and characterization of polycaprolactone/chitosan nanofibers containing antibacterial agents of curcumin and ZnO nanoparticles for use as wound dressing

Novel Scaffold Based on Chitosan Hydrogels/Phthalated Cashew Gum for Supporting Human Dental Pulp Stem Cells

Cellulose-Based Scaffolds: A Comparative Study for Potential Application in Articular Cartilage

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