Polycaprolactone/chlorinated bioglass scaffolds doped with Mg and Li ions: Morphological, physicochemical, and biological analysis

Kukulka, Elisa Camargo; Souza, Joyce Rodrigues de; Araújo, Juliani Carolini Ribeiro de; Vasconcellos, Luana Marotta Reis de; Campos, Tiago Moreira Bastos; Thim, Gilmar Patricínio; Borges, Alexandre Luiz Souto

doi:10.1002/jbm.b.35140

Cited by 7 publications

(5 citation statements)

References 53 publications

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“…This observation indicated a notable impact of the incorporation of BG on the fiber size. SEM images (Figure 3B) further support these findings, indicating the influence of bioglass on fiber structure, which is in agreement with the study conducted by Kukulka et al 41 This could potentially lead to increased surface area for cell attachment and proliferation. SEM images (Figure 3B) further support these findings, indicating the influence of bioglass on fiber structure.…”

Section: Discussionsupporting

confidence: 91%

“…Sodium metasilicate (0.65 mol/L) underwent ion exchange resin treatment to form silicic acid (Si[OH] 4 ), serving as the silica precursor. 14,[19][20][21][22][23] In the production of 45S5 bioglass, Si(OH) 4 was blended with calcium chloride hydrate (CaCl 2 ), ammonium phosphate monobasic (NH 4 H 2 PO 4 ), and sodium chloride (NaCl). The resulting solution was subjected to drying at 100 C for 24 h, followed by freezing and subsequent freeze-drying for 72 h. Following the lyophilization process, the sample was calcined at 600 C for 5 h, resulting in the formation of a calcined and lyophilized silicate chlorinated BG.…”

Section: Synthesis Of 45s5 Bioglass By the Sol-gel Methodsmentioning

confidence: 99%

“…The synthesis of bioglass 45S5 (composed of wt % 45% SiO 2 ; 24.5% CaO; 24.5% Na 2 O; and 6% P 2 O 5 ) followed a sol–gel procedure. Sodium metasilicate (0.65 mol/L) underwent ion exchange resin treatment to form silicic acid (Si[OH] 4 ), serving as the silica precursor 14,19–23 . In the production of 45S5 bioglass, Si(OH) 4 was blended with calcium chloride hydrate (CaCl 2 ), ammonium phosphate monobasic (NH 4 H 2 PO 4 ), and sodium chloride (NaCl).…”

Section: Methodsmentioning

confidence: 99%

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Biodegradable electrospun poly(L‐lactide‐co‐ε‐caprolactone)/polyethylene glycol/bioactive glass composite scaffold for bone tissue engineering

de Souza,

Cardoso,

de Toledo

et al. 2024

J Biomed Mater Res

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The field of tissue engineering has witnessed significant advancements in recent years, driven by the pursuit of innovative solutions to address the challenges of bone regeneration. In this study, we developed an electrospun composite scaffold for bone tissue engineering. The composite scaffold is made of a blend of poly(L‐lactide‐co‐ε‐caprolactone) (PLCL) and polyethylene glycol (PEG), with the incorporation of calcined and lyophilized silicate‐chlorinated bioactive glass (BG) particles. Our investigation involved a comprehensive characterization of the scaffold's physical, chemical, and mechanical properties, alongside an evaluation of its biological efficacy employing alveolar bone‐derived mesenchymal stem cells. The incorporation of PEG and BG resulted in elevated swelling ratios, consequently enhancing hydrophilicity. Thermal gravimetric analysis confirmed the efficient incorporation of BG, with the scaffolds demonstrating thermal stability up to 250°C. Mechanical testing revealed enhanced tensile strength and Young's modulus in the presence of BG; however, the elongation at break decreased. Cell viability assays demonstrated improved cytocompatibility, especially in the PLCL/PEG+BG group. Alizarin red staining indicated enhanced osteoinductive potential, and fluorescence analysis confirmed increased cell adhesion in the PLCL/PEG+BG group. Our findings suggest that the PLCL/PEG/BG composite scaffold holds promise as an advanced biomaterial for bone tissue engineering.

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Section: Discussionsupporting

confidence: 91%

Section: Synthesis Of 45s5 Bioglass By the Sol-gel Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Biodegradable electrospun poly(L‐lactide‐co‐ε‐caprolactone)/polyethylene glycol/bioactive glass composite scaffold for bone tissue engineering

de Souza,

Cardoso,

de Toledo

et al. 2024

J Biomed Mater Res

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“…The dispersion utilized for the electrospinning consisted of 11% PCL dissolved in acetone (w/v) and 13.5% of bioceramic. The components were mixed at a constant rotation of 300 rpm 16 on a shaker (Solab, SP, Brazil) until the total dissolution of the polymer. Thus, three solutions were obtained: PCL (control), PCL/N600, and PCL/CL600.…”

Section: Methodsmentioning

confidence: 99%

Chlorinated‐based bioceramics incorporated in polycaprolactone membranes

Guimarães,

de Souza,

Campos

et al. 2023

J Biomed Mater Res

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The development of bioactive membranes with bone repair properties is great interest in the field of tissue engineering. In this study, we aimed to fabricate and characterize a composite membrane composed of sol–gel synthesized bioceramics and electrospun polycaprolactone (PCL) fibers for bone tissue regeneration applications. The bioceramics were prepared using the sol–gel method with nitrate (N) and chloride (CL) as precursors. PCL and bioceramic solutions were electrospun to obtain ultrafine fiber mats. Raman spectroscopy, x‐ray diffraction (XRD), Fourier Transform Infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM) were used to characterize the materials. The results showed that both chlorinated and non‐chlorinated bioceramics contained NBOs (non‐bridge bonds) and crystallized the α‐wollastonite phase, with the chlorinated version doing so at lower temperatures. In vitro tests were performed to evaluate cytotoxicity, cell adhesion, and mineralized matrix formation on the membranes. The composite membranes showed improved cell viability and promoted mineralization nodules formation. This study presents a promising approach for the development of bioactive membranes for bone tissue engineering, with potential applications in bone regeneration therapies.

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“…BG has been combined with many different materials, for example, various hydrogels [ 8 ] , graphene oxide [ 9 ] , or polycaprolactone [ 10 ] , from which it is released and induces osteogenic properties [ 11 ] . By combining PLA and bioglass, it might be possible to combine the positive characteristics of these materials and overcome their negative aspects.…”

Section: Introductionmentioning

confidence: 99%

A new 3D-printed polylactic acid-bioglass composite for bone tissue engineering induces angiogenesis in vitro and in ovo

Cichos,

Schätzlein,

Wiesmann-Imilowski

et al. 2023

IJB

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Large bone defects such as those that occur after trauma or resections due to cancer still are a challenge for surgeons. Main challenge in this area is to find a suitable alternative to the gold-standard therapy, which is highly risky, and a promising option is to use biomaterials manufactured by 3D printing. In former studies, we demonstrated that the combination of polylactic acid (PLA) and bioglass (BG) resulted in a stable 3D-printable material, and porous and finely structured scaffolds were printed. These scaffolds exhibited osteogenic and anti-inflammatory properties. This 3D-printed material fulfills most of the requirements described in the diamond concept of bone healing. However, the question remains as to whether it also meets the requirements concerning angiogenesis. Therefore, the aim of this study was to analyze the effects of the 3D-printed PLA-BG composite material on angiogenesis. In vitro analyses with human umbilical vein endothelial cells (HUVECs) showed a positive effect of increasing BG content on viability and gene expression of endothelial markers. This positive effect was confirmed by an enhanced vascular formation analyzed by Matrigel assay and chicken chorioallantoic membrane (CAM) assay. In this work, we demonstrated the angiogenic efficiency of a 3D-printed PLA–BG composite material. Recalling the osteogenic potential of this material demonstrated in former work, we manufactured a mechanically stable, 3D-printable, osteogenic and angiogenic material, which could be used for bone tissue engineering.

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Polycaprolactone/chlorinated bioglass scaffolds doped with Mg and Li ions: Morphological, physicochemical, and biological analysis

Cited by 7 publications

References 53 publications

Biodegradable electrospun poly(L‐lactide‐co‐ε‐caprolactone)/polyethylene glycol/bioactive glass composite scaffold for bone tissue engineering

Biodegradable electrospun poly(L‐lactide‐co‐ε‐caprolactone)/polyethylene glycol/bioactive glass composite scaffold for bone tissue engineering

Chlorinated‐based bioceramics incorporated in polycaprolactone membranes

A new 3D-printed polylactic acid-bioglass composite for bone tissue engineering induces angiogenesis in vitro and in ovo

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