Polycaprolactone Composite Micro/Nanofibrous Material as an Alternative to Restricted Access Media for Direct Extraction and Separation of Non-Steroidal Anti-Inflammatory Drugs from Human Serum Using Column-Switching Chromatography

Abstract

Application of the poly-ɛ-caprolactone composite sorbent consisting of the micro- and nanometer fibers for the on-line extraction of non-steroidal anti-inflammatory drugs from a biological matrix has been introduced. A 100 μL human serum sample spiked with ketoprofen, naproxen, sodium diclofenac, and indomethacin was directly injected in the extraction cartridge filled with the poly-ɛ-caprolactone composite sorbent. This cartridge was coupled with a chromatographic instrument via a six-port switching valve allowing the analyte extraction and separation within a single analytical run. The 1.5 min long extraction step isolated the analytes from the proteinaceous matrix was followed by their 13 min HPLC separation using Ascentis Express RP-Amide (100 × 4.6 mm, 5 µm) column. The recovery of all analytes from human serum tested at three concentration levels ranged from 70.1% to 118.7%. The matrix calibrations were carried out in the range 50 to 20,000 ng mL−1 with correlation coefficients exceeding 0.996. The detection limit was 15 ng mL−1, and the limit of quantification corresponded to 50 ng mL−1. The developed method was validated and successfully applied for the sodium diclofenac determination in real patient serum. Our study confirmed the ability of the poly-ɛ-caprolactone composite sorbent to remove the proteins from the biological matrix, thus serving as an alternative to the application of restricted-access media.

New Challenges in (Bio)Analytical Sample Treatment Procedures for Clinical Applications

“…Raabova et al used human serum taken from patients that were under treatment of intravenous infusion with 75 mg of sodium diclofenac. The blood samples were diluted 10 times with 20% aqueous acetonitrile, centrifuged, and the supernatant was analyzed [30].…”

Special Issue “Nanomaterials for Biomedical and Biotechnological Applications”

“…Concerning the chemistry application of nanomaterials, the theme of complex biological mixture separation is discussed by Raabová and colleagues [ 12 ]. In their research paper, the authors describe the advantages of combining poly-ε-caprolactone micro- and nanofibers for the online extraction of analytes from milk and serum matrixes, and confirm the protein removal capability, good mechanical stability and re-usability of this sorbent in more than 300 analyses.…”