Polycaprolactone nanofibers functionalized with placental derived extracellular matrix for stimulating wound healing activity

Rameshbabu, Arun Prabhu; Datta, Sayanti; Bankoti, Kamakshi; Subramani, Elavarasan; Chaudhury, Koel; Lalzawmliana, V.; Nandi, Samit Kumar

doi:10.1039/c8tb01373j

Cited by 52 publications

(36 citation statements)

References 63 publications

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“…Other bioactive molecules can also trigger angiogenesis including metallic ions, platelet-rich plasma (PRP) [158,159], ECM derivatives, recombinant proteins [160][161][162][163], and decellularized matrices [164]. Plenty of experimental studies have been conducted to survey the feasibility of such approaches to promote angiogenesis.…”

Section: Nanofibers Incorporating Phytochemicals and Other Bioactive mentioning

confidence: 99%

“…Angiogenesis plays a pivotal role in soft tissue wound healing, especially in the case of chronic wounds [252,253]. Several electrospun membranes have shown great abilities to improve the healing process of epidermal and dermal layers [163,254]. By highlighting the role of angiogenesis in the skin regeneration process, a multifunctional and biomimetic nanofibrous membrane was composed of fish collagen type I (COL) and bioactive glasses (BGs) using the electrospinning method [255].…”

Section: Angiogenic Nanofibrous Mats For Skin Regenerationmentioning

confidence: 99%

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Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

et al. 2020

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Angiogenesis (or the development of new blood vessels) is a key event in tissue engineering and regenerative medicine; thus, a number of biomaterials have been developed and combined with stem cells and/or bioactive molecules to produce three-dimensional (3D) pro-angiogenic constructs. Among the various biomaterials, electrospun nanofibrous scaffolds offer great opportunities for pro-angiogenic approaches in tissue repair and regeneration. Nanofibers made of natural and synthetic polymers are often used to incorporate bioactive components (e.g., bioactive glasses (BGs)) and load biomolecules (e.g., vascular endothelial growth factor (VEGF)) that exert pro-angiogenic activity. Furthermore, seeding of specific types of stem cells (e.g., endothelial progenitor cells) onto nanofibrous scaffolds is considered as a valuable alternative for inducing angiogenesis. The effectiveness of these strategies has been extensively examined both in vitro and in vivo and the outcomes have shown promise in the reconstruction of hard and soft tissues (mainly bone and skin, respectively). However, the translational of electrospun scaffolds with pro-angiogenic molecules or cells is only at its beginning, requiring more research to prove their usefulness in the repair and regeneration of other highly-vascularized vital tissues and organs. This review will cover the latest progress in designing and developing pro-angiogenic electrospun nanofibers and evaluate their usefulness in a tissue engineering and regenerative medicine setting.

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Section: Nanofibers Incorporating Phytochemicals and Other Bioactive mentioning

confidence: 99%

Section: Angiogenic Nanofibrous Mats For Skin Regenerationmentioning

confidence: 99%

Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

et al. 2020

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“…Since the exact distribution of both components in fi bres has not been investigated yet, the infl uence of the fl uid culture medium on fi bre integrity has to be examined. Generally, nanofi brous mats prepared from biopolymers, such as gelatine or alginate, are known to support cell growth for wound healing, tissue engineering or biotechnological purposes [15][16][17][18][19]. Especially for elongated cells, such as neurites or fi broblasts, nanofi bres have been shown to support cell growth [20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Sterilization of PAN/Gelatine Nanofibrous Mats for Cell Growth

Wehlage¹,

Blattner²,

Sabantina³

et al. 2019

TEK

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Nanofi brous mats can be used as a substrate for eukaryotic cell growth in biotechnology, tissue engineering, etc. Several adherent cells (e.g. human fi broblasts) have been shown to grow well on fi ne fi bres. For most applications, it is necessary to sterilize nanofi brous mats before adding the cells. Another possibility would be the addition of antibiotics and antimycotics to the cell culture medium to prevent microbial infection. However, antibiotics are disadvantageous since they might promote the growth of resistant bacteria in possible future medical applications of nanofi brous mats. Possible sterilization techniques include autoclaving, UV-sterilization, ozone treatment, heat sterilization and other techniques which usually necessitate more expensive equipment, such as gamma irradiation. Systematic examinations of the infl uence of diff erent sterilization techniques on the cell growth on nanofi brous mats have not yet been reported in the literature. Here, we report on the fi rst experimental investigations of the eff ect of sterilization with diff erent methods on the properties of polyacrylonitrile (PAN)/gelatine nanofi brous mats, and the resulting growth and adhesion of Chinese hamster ovary cells. While all techniques under investigation yielded sterile nanofibrous mats, autoclaving and heat sterilization change the PAN/gelatine fi bre morphology. Ozone, on the other hand, modifi es the pH value of the culture medium and partly impedes cell adhesion. UV sterilization also suggests a chemical modifi cation of the nanofi brous mat. Unexpectedly, heat sterilization resulted in the highest amount of adherent Chinese hamster ovary cells grown on PAN/gelatine nanofi brous mats in spite of gelatine melting.

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“…Polycaprolactone is another biocompatible and biodegradable polymer that has a low melting point and glass transition temperature. PCL is highly soluble in organic solvents, such as hexafluoroisopropanol, dichloromethane, chloroform, methanol, tetrahydrofuran, and others (Rameshbabu et al, 2018); it is therefore more suitable when longer degradation times are required. PCL variants with more hydrophilic monomers promote more rapid water diffusion, thus increasing the degradation rate (Guarino et al, 2017).…”

Section: Synthetic Polymersmentioning

confidence: 99%

Electrospun Nanofibers as Carriers of Microorganisms, Stem Cells, Proteins, and Nucleic Acids in Therapeutic and Other Applications

Stojanov

Berlec

2020

Front. Bioeng. Biotechnol.

129

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Electrospinning is a technique that uses polymer solutions and strong electric fields to produce nano-sized fibers that have wide-ranging applications. We present here an overview of the use of electrospinning to incorporate biological products into nanofibers, including microorganisms, cells, proteins, and nucleic acids. Although the conditions used during electrospinning limit the already problematic viability/stability of such biological products, their effective incorporation into nanofibers has been shown to be feasible. Synthetic polymers have been more frequently applied to make nanofibers than natural polymers. Polymer blends are commonly used to achieve favorable physical properties of nanofibers. The majority of nanofibers that contain biological product have been designed for therapeutic applications. The incorporation of these biological products into nanofibers can promote their stability or viability, and also allow their delivery to a desired tissue or organ. Other applications include plant protection in agriculture, fermentation in the food industry, biocatalytic environmental remediation, and biosensing. Live cells that have been incorporated into nanofibers include bacteria and fungi. Nanofibers have served as scaffolds for stem cells seeded on a surface, to enable their delivery and application in tissue regeneration and wound healing. Viruses incorporated into nanofibers have been used in gene delivery, as well as in therapies against bacterial infections and cancers. Proteins (hormones, growth factors, and enzymes) and nucleic acids (DNA and RNA) have been incorporated into nanofibers, mainly to treat diseases and enhance their stability. To summarize, incorporation of biological products into nanofibers has numerous advantages, such as providing protection and facilitating controlled delivery from a solid form with a large surface area. Future studies should address the challenge of transferring nanofibers with biological products into practical and industrial use.

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Polycaprolactone nanofibers functionalized with placental derived extracellular matrix for stimulating wound healing activity

Abstract: Impaired wound healing is primarily associated with inadequate angiogenesis, repressed cell migration, deficient synthesis of extracellular matrix (ECM) component/growth factors, and altered inflammatory responses in the wound bed environment.

Cited by 52 publications

References 63 publications

Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

Sterilization of PAN/Gelatine Nanofibrous Mats for Cell Growth

Electrospun Nanofibers as Carriers of Microorganisms, Stem Cells, Proteins, and Nucleic Acids in Therapeutic and Other Applications

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