Polycation-based DNA complexes for tumor-targeted gene delivery in vivo

Kircheis, Ralf; Schüller, Susanne; Brunner, Sylvia; Ogris, Manfred; Heider, Karl-Heinz; Zauner, Wolfgang; Wagner, Ernst

doi:10.1002/(sici)1521-2254(199903/04)1:2<111::aid-jgm22>3.3.co;2-p

Cited by 75 publications

(98 citation statements)

References 12 publications

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“…Following intravenous application in SCID mice, analysis of luciferase distribution in both models showed that expression levels within the tumors were up to 2 logs higher than levels found in the liver, which was the highest expressing major organ, with expression in the tumor accounting for 97% to 99% of the total transgene expression measured in vivo. Notably, no acute toxicity, as reported for positively charged DNA/PEI particles, 13,14 was observed.…”

Section: Discussionmentioning

confidence: 99%

Specific systemic nonviral gene delivery to human hepatocellular carcinoma xenografts in SCID mice

et al. 2002

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Section: Discussionmentioning

confidence: 99%

Specific systemic nonviral gene delivery to human hepatocellular carcinoma xenografts in SCID mice

et al. 2002

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“…The reasons for the differences remain to be clarified; reporter gene expression may not be in direct correlation to the body distribution pattern, as previously described. 24,25 In the current case, positive charges of polyplexes cannot be the sole explanation for the observed gene expression patterns since HD O at c/p ratio of 2 still shows a more favorable tumor/lung relation as compared to LPEI.…”

Section: Gene Expression In Vivomentioning

confidence: 99%

Novel degradable oligoethylenimine acrylate ester-based pseudodendrimers for in vitro and in vivo gene transfer

et al. 2007

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“…16,17 Intravenous administration of genes in mice mediated by PEGylated Tf-PEI/DNA complexes could result in a high expression of exogenous genes in tumor but in tail as well. 18 Here, we described the successful gene transfer into subcutaneously transplanted tumors via systemic administration of the GE7 system. Our results demonstrated that high b-gal activity could be observed in tumor tissues and the expression there persisted for more than 2 weeks.…”

Section: Discussionmentioning

confidence: 99%

Systemic genetic transfer of p21WAF−1 and GM-CSF utilizing of a novel oligopeptide-based EGF receptor targeting polyplex

Liu

Tian

et al. 2003

Cancer Gene Ther

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Limited.Based on the fact that aberrant overexpression of some growth factor receptors was observed in a variety of human cancer cells, a novel nonviral gene delivery system GE7, which contains a 16-amino-acid ligand for identifying EGF receptor was constructed for tumor-targeted gene therapy. Intravenous administration of GE7 system revealed that it has the ability to target b-galactosidase (b-gal ) reporter gene into murine hepatoma (Hepa) cells. Owing to the limited antitumor effects elicited by a single-gene transfer, recent efforts to treat malignancy using combined gene therapy have been accomplished with varying degrees of success. In this study, the human cyclin-dependent kinase inhibitor gene p21 WAFÀ1 and the murine cytokine gene granulocyte-macrophage colony-stimulating factor (GM-CSF) were used simultaneously for in vivo gene therapy through systemic injection of the EGF R targeted GE7/DNA complex into murine hepatoma-bearing mice. The results demonstrated that combined administration of p21 WAFÀ1 and GM-CSF could remarkably inhibit the growth of subcutaneously transplanted hepatoma Hepa cells, and significantly increase the survival rate of tumor-bearing mice. The activities of natural killer (NK) cells and specific cytotoxic T lymphocytes (CTL) were clearly enhanced after combined gene therapy. In vitro experiments showed that p21 WAFÀ1 gene transfer exhibited a suppressive function on the growth of Hepa cells and the expression of H-2K b and B7-1 molecules on Hepa cells increased significantly after combined genes delivery. All these results suggested that the GE7 system was able to target therapeutic genes efficiently to cancer cells, which showed high EGF R expression. The cotransfer of p21 WAFÀ1 and GM-CSF genes apparently inhibited the growth of tumors through (a) the arrest of tumor cell growth and (b) the enhancement of systemic antitumor immunity.

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Polycation-based DNA complexes for tumor-targeted gene delivery in vivo

Cited by 75 publications

References 12 publications

Specific systemic nonviral gene delivery to human hepatocellular carcinoma xenografts in SCID mice

Specific systemic nonviral gene delivery to human hepatocellular carcinoma xenografts in SCID mice

Novel degradable oligoethylenimine acrylate ester-based pseudodendrimers for in vitro and in vivo gene transfer

Systemic genetic transfer of p21WAF−1 and GM-CSF utilizing of a novel oligopeptide-based EGF receptor targeting polyplex

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