Polychaete burrows harbour distinct microbial communities in oil‐contaminated coastal sediments

Taylor, John D.; Cunliffe, Michael

doi:10.1111/1758-2229.12292

Cited by 23 publications

(21 citation statements)

References 34 publications

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“…Several studies have shown that fungi are abundant in marine sediments (Orsi et al, 2013a, b;Xu et al, 2014;Taylor and Cunliffe, 2015). Xu et al (2014) used a similar Q-PCR-based approach to quantify fungi in sediment samples collected from five deep-sea sites in the Pacific Ocean and showed that Fungi-specific 28S rRNA gene copies ranged from 3.5 × 10 6 to 5.2 × 10 7 copies per g wet sediment (Xu et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Multi-year assessment of coastal planktonic fungi reveals environmental drivers of diversity and abundance

2016

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Mycoplankton have so far been a neglected component of pelagic marine ecosystems, having been poorly studied relative to other plankton groups. Currently, there is a lack of understanding of how mycoplankton diversity changes through time, and the identity of controlling environmental drivers. Using Fungi-specific high-throughput sequencing and quantitative PCR analysis of plankton DNA samples collected over 6 years from the coastal biodiversity time series site Station L4 situated off Plymouth (UK), we have assessed changes in the temporal variability of mycoplankton diversity and abundance in relation to co-occurring environmental variables. Mycoplankton diversity at Station L4 was dominated by Ascomycota, Basidiomycota and Chytridiomycota, with several orders within these phyla frequently abundant and dominant in multiple years. Repeating interannual mycoplankton blooms were linked to potential controlling environmental drivers, including nitrogen availability and temperature. Specific relationships between mycoplankton and other plankton groups were also identified, with seasonal chytrid blooms matching diatom blooms in consecutive years. Mycoplankton α-diversity was greatest during periods of reduced salinity at Station L4, indicative of riverine input to the ecosystem. Mycoplankton abundance also increased during periods of reduced salinity, and when potential substrate availability was increased, including particulate organic matter. This study has identified possible controlling environmental drivers of mycoplankton diversity and abundance in a coastal sea ecosystem, and therefore sheds new light on the biology and ecology of an enigmatic marine plankton group. Mycoplankton have several potential functional roles, including saprotrophs and parasites, that should now be considered within the consensus view of pelagic ecosystem functioning and services.

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Section: Discussionmentioning

confidence: 99%

Multi-year assessment of coastal planktonic fungi reveals environmental drivers of diversity and abundance

2016

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“…After 18 h, 1 l seawater samples were removed, filtered through 0.2 µm filters and the filters stored at −80°C. RNA was extracted from the filters as previously described (Taylor and Cunliffe, ) and cDNA generation was performed using the Omniscript RT kit (Qiagen) in accordance with manufacturer's instructions using the reverse primer PROK1492R (Suzuki et al . ).…”

Section: Introductionmentioning

confidence: 99%

“…After 18 h, 1 l seawater samples were removed, filtered through 0.2 mm filters and the filters stored at 2808C. RNA was extracted from the filters as previously described (Taylor and Cunliffe, 2015) and cDNA generation was performed using the Omniscript RT kit (Qiagen) in accordance with manufacturer's instructions using the reverse primer PROK1492R (Suzuki et al 2000). The V4 region of the 16S rRNA gene was amplified by PCR using primers 515F and 806R (Caporaso et al, 2011) from the cDNA and sequenced on an Ion Torrent PGM (Life Technologies) in accordance with manufacturer's instructions.…”

Section: Introductionmentioning

confidence: 99%

Coastal bacterioplankton community response to diatom‐derived polysaccharide microgels

Taylor

Cunliffe

2017

Environ Microbiol Rep

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Phytoplankton-derived polysaccharide microgels, including transparent exopolymer particles (TEP), are a major component of the marine organic carbon pool. Previous studies have made correlative links between phytoplankton material and bacterioplankton, and performed experiments that assess general responses to phytoplankton, yet there is a lack of direct empirical evidence of specific bacterioplankton responses to natural phytoplankton polysaccharide microgels. In this study, we used diatom produced TEP in controlled incubation experiments to determine the impact of polysaccharide microgels on a coastal bacterioplankton community. Quantification of bacterial 16S rRNA gene transcripts showed that the addition of TEP caused an increase in bacterioplankton activity. Similarly, high-throughput sequencing of RT-PCR amplified bacterial 16S rRNA gene transcripts showed that active bacterioplankton community structure and diversity also changed in response to microgels. Alteromonadales and Rhodobacterales increased in abundance in response to TEP, suggesting that both bacterioplankton taxa utilize diatom-derived microgels. However, through assessing C-labelled TEP uptake via RNA Stable Isotope Probing, we show that only the Alteromonadales (genus Alteromonas) assimilated the TEP carbon. This study adds utilization of diatom-derived TEP to the metabolic repertoire of the archetypal copiotrophic bacterioplankton Alteromonas, and indicates that the Rhodobacterales may utilize TEP for other purposes (e.g. attachment sites).

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“…; Pinto & Bemvenuti ; Rosa & Bemvenuti ; Shimanaga et al . ; Taylor & Cunliffe ). The cylindrical morphology and the trajectory of Permian H. tenuis suggest the displacement of a nematodelike organism within the substrate.…”

Section: Discussionmentioning

confidence: 99%

Permian macroburrows as microhabitats for meiofauna organisms: an ancient behaviour common in extant organisms

Villegas‐Martín

Netto

2019

LET

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Meiofaunal organisms are indirectly influenced by the activity of benthic macroinvertebrates within the sediment, which plays a role in modifying physical and chemical characteristics of the habitat. The association of meiofaunal organisms and macroburrows is well known in modern environments, but the record of this relationship in the geological record is still incipient. This study documents diminutive burrows (Helminthoidichnites tenuis) associated with the surface of macroburrows (Palaeophycus tubularis) in Early Permian deposits. The cylindrical shape and meandering to loop trajectory of the diminutive burrows indicate that they were produced by small free‐living meiofaunal nematodes. Apparently, P. tubularis (open burrow) constituted a favourable microhabitat for nematodes, providing the following: (1) protection against erosive processes and meiofauna predators; (2) oxygen access to more in‐depth layers within the sediment; (3) temperature stabilization; and (4) food supply due to mucus impregnation in the macroburrow walls by the Palaeophycus tracemaker. The association between H. tenuis and P. tubularis constitutes the first fossil record of a symbiotic relationship between meiofaunal nematodes and macrobenthic organisms (polychaetes). It also suggests that ecological strategies such as mutualism or commensalism, which are common between extant nematodes and macrobenthic invertebrates, were available in the behavioural programme of these organisms since the Early Permian.

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Polychaete burrows harbour distinct microbial communities in oil‐contaminated coastal sediments

Cited by 23 publications

References 34 publications

Multi-year assessment of coastal planktonic fungi reveals environmental drivers of diversity and abundance

Multi-year assessment of coastal planktonic fungi reveals environmental drivers of diversity and abundance

Coastal bacterioplankton community response to diatom‐derived polysaccharide microgels

Permian macroburrows as microhabitats for meiofauna organisms: an ancient behaviour common in extant organisms

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