Polychlorinated biphenyls and methylmercury act synergistically to reduce rat brain dopamine content in vitro.

Bemis, Jeffrey C.; Seegal, Richard F.

doi:10.1289/ehp.99107879

Cited by 136 publications

(54 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The elevations in dialysate DA concentrations seen here are strikingly similar to those seen in media DA concentrations after acute ex vivo exposure of adult rat striatal tissue to Aroclor mixtures (Bemis and Seegal 1999;Chishti et al 1996) and are likely to reflect similar mechanisms. Indeed, in those reports, we suggested that the elevations in media DA may have been due to PCB-induced inhibition of DA transporters.…”

Section: Discussionsupporting

confidence: 74%

“…Using pheochromocytoma (PC12) cells, we demonstrated that only noncoplanar PCB congeners significantly decreased cell DA content (Shain et al 1991). Similar decreases in tissue DA content were described by Chishti et al (1996) and by Bemis and Seegal (1999) after ex vivo exposure of striatal slices from adult rats to commercial mixtures of PCBs. These decreases in tissue DA content after acute exposure were accompanied by significant elevations in media DA, which led us to suggest that PCBs either enhance release of DA and/or inhibit transport of the neurotransmitter back into the neuron.…”

supporting

confidence: 86%

“…Concentrations of DA, in either microdialysates or perchloric acid supernatants of striatal tissue homogenates, were determined using chromatographic separation methods developed and/or modified in our laboratory (Bemis and Seegal 1999;Nakamura et al 1992;Seegal et al 1986a). Aliquots (20 µL) of striatal microdialysates or of a 1:10 dilution of striatal tissue homogenates were injected via a Waters 717 refrigerated autosampler (Waters Corp., Milford, MA) onto a SUPELCOSIL LC-18-DB 25 cm × 4.6 mm, 5-µm C18 reverse-phase column (Supelco, Bellefonte, PA) maintained at 35°C.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Polychlorinated biphenyls alter extraneuronal but not tissue dopamine concentrations in adult rat striatum: an in vivo microdialysis study.

Seegal

Okoniewski

Brosch

et al. 2002

Environ Health Perspect

View full text Add to dashboard Cite

Polychlorinated biphenyls (PCBs) reduce tissue dopamine (DA) concentrations and increase media DA concentrations in both in vitro preparations of bovine adrenal chromaffin cells and adult rat striatal tissue. To determine whether these changes also occur in the intact animal, we used in vivo microdialysis to determine changes in concentrations of DA in striatal dialysates from freely moving adult male rats after exposure to 25 mg/kg/day Aroclor 1254 for varying periods of time. We also determined DA concentrations in striatal tissue obtained postmortem from similarly treated animals. The effects of PCBs on dialysate DA concentrations depended on the length of exposure; DA concentrations were significantly elevated after 3 days of exposure and were significantly reduced after exposure for periods of 1 week or longer. On the other hand, striatal tissue concentrations of DA, determined postmortem in rats exposed to PCBs for the same periods of time, were not significantly altered. We suggest that these time-dependent alterations in dialysate DA concentrations a) reflect PCB-induced alterations of both plasma membrane and vesicular DA transporter function; b) provide a more sensitive index of altered central DA function after exposure to PCBs than does measurement of postmortem tissue DA concentrations; and c) play an important role in mediating some PCB-mediated changes in behavior.

show abstract

Section: Discussionsupporting

confidence: 74%

supporting

confidence: 86%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Polychlorinated biphenyls alter extraneuronal but not tissue dopamine concentrations in adult rat striatum: an in vivo microdialysis study.

Seegal

Okoniewski

Brosch

et al. 2002

Environ Health Perspect

View full text Add to dashboard Cite

show abstract

“…However, adverse developmental effects of MeHg associated with seafood consumption have not been observed consistently (22,25) . Some evidence suggests PCB, which have been measured at fairly high concentrations in samples of seafood from Spain, may potentiate adverse developmental effects of MeHg (11,(49)(50)(51)(52) . It has also been suggested that adverse neurodevelopmental effects of PCB may be lower in breast-fed children (38) .…”

Section: Discussionmentioning

confidence: 99%

Maternal fish and other seafood intakes during pregnancy and child neurodevelopment at age 4 years

Mendez

Torrent

Júlvez

et al. 2009

Public Health Nutr.

View full text Add to dashboard Cite

Objective: To analyse the relationship between maternal intakes of fish and other seafood during pregnancy and child neurodevelopment at age 4 years. Although pregnant women are advised to limit seafood intakes because of possible neurotoxin contamination, several studies suggest that overall maternal seafood intakes are associated with improved child neurodevelopment, perhaps because of higher DHA intakes. Design: The study uses data from a prospective birth cohort study. Maternal seafood intakes were assessed using a semi-quantitative FFQ administered shortly after delivery. Multivariate linear regression was used to estimate associations between seafood consumption and scores on the McCarthy Scales of Children's Abilities (MCSA). Analyses were stratified by breast-feeding duration as breast milk is a source of DHA during the postnatal phase of the brain growth spurt. Setting: Menorca, Spain, 1997-2001. Subjects: Full-term children (n 392) with data on maternal diet in pregnancy, breast-feeding duration and neurodevelopment at age 4 years. Results: Among children breast-fed for ,6 months, maternal fish intakes of .2-3 times/week were associated with significantly higher scores on several MCSA subscales compared with intakes #1 time/week. There was no association among children breast-fed for longer periods. Maternal intakes of other seafood (shellfish/squid) were, however, inversely associated with scores on several subscales, regardless of breast-feeding duration.Conclusions: The study suggests that moderately high intakes of fish, but not other seafood, during pregnancy may be beneficial for neurodevelopment among children breast-fed for ,6 months. Further research in other populations with high seafood intakes and data on additional potential confounders are needed to confirm this finding.

show abstract

“…A cross-sectional cut at the level of the hypothalamus was made and the rostral portion was placed in ice-cold HBHS. Several 350-μM thick slices containing the striatum were collected, processed, and prepared for Mn exposure according to Chishti et al (1996) and Bemis and Seegal (1999). In all cases, striatal slices were incubated in 24-well plates containing 500 μl of 1% HBHS/well and the indicated working concentrations of Mn.…”

Section: Striatal Tissue Experimentsmentioning

confidence: 99%

Direct effects of manganese compounds on dopamine and its metabolite Dopac: An in vitro study

Sistrunk

Ross

Filipov

2007

Environmental Toxicology and Pharmacology

View full text Add to dashboard Cite

Following combustion of fuel containing the additive methylcyclopentadienyl-manganesetricarbonyl (MMT), manganese phosphate (MnPO 4 ) and manganese sulfate (MnSO 4 ) are emitted in the atmosphere. Manganese chloride (MnCl 2 ), another Mn 2+ species, is widely used experimentally. Using rat striatal slices, we found that MnPO 4 decreased tissue and media dopamine (DA) and media Dopac (a DA metabolite) levels substantially more than either MnCl 2 or MnSO 4 ; antioxidants were partially protective. Also, both MnCl 2 and MnPO 4 (more potently) oxidized DA and Dopac even in the absence of tissue in the media, suggesting a direct interaction between Mn and DA/Dopac. Because aminochrome is a major oxidation product of DA, we next determined whether MnPO 4 will be more potent in forming aminochrome than MnCl 2 or MnSO 4 which, indeed, was the case. Thus, a potential additional mechanism for the neurotoxic effects of environmentally-relevant forms of Mn, MnPO 4 in particular, is the generation of reactive DA intermediates.

show abstract

Polychlorinated biphenyls and methylmercury act synergistically to reduce rat brain dopamine content in vitro.

Cited by 136 publications

References 31 publications

Polychlorinated biphenyls alter extraneuronal but not tissue dopamine concentrations in adult rat striatum: an in vivo microdialysis study.

Polychlorinated biphenyls alter extraneuronal but not tissue dopamine concentrations in adult rat striatum: an in vivo microdialysis study.

Maternal fish and other seafood intakes during pregnancy and child neurodevelopment at age 4 years

Direct effects of manganese compounds on dopamine and its metabolite Dopac: An in vitro study

Contact Info

Product

Resources

About