Polyclonal B-Cell activation byNeisseria meningitidis capsular polysaccharides elicit antibodies protective againstTrypanosoma cruzi infection in vitro

Oliveira, Teresa G.; Milani, S.; Travassos, Luiz R.

doi:10.1002/(sici)1098-2825(1996)10:4<220::aid-jcla8>3.0.co;2-d

Cited by 6 publications

(5 citation statements)

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“…It therefore appears that either there is a degree of selection for incorporation into the HLA‐DR – compartment, or that there are certain common antigens that all individuals are exposed to during the course of their lifetime and sequences against these common antigens dominate the HLA‐DR – compartment. Another possibility is that in addition to vaccine‐specific PCs in the HLA‐DR + population, additional inter‐individual variation arises from the presence of PCs generated in incidental responses against non‐vaccine antigens or because of ‘bystander stimulation’ 39 . Perhaps the most compelling evidence we have of the HLA‐DR + and HLA‐DR – PCs comprising distinct populations is that when analyzing, which baseline B‐cell subsets are activated to produce the IgG PCs, we see differences between the vaccine groups for the HLA‐DR + , but not the HLA‐DR – PCs.…”

Section: Discussionmentioning

confidence: 99%

“…Another possibility is that in addition to vaccine-specific PCs in the HLA-DR + population, additional inter-individual variation arises from the presence of PCs generated in incidental responses against non-vaccine antigens or because of 'bystander stimulation'. 39 Perhaps the most compelling evidence we have of the HLA-DR + and HLA-DR -PCs comprising distinct populations is that when analyzing, which baseline B-cell subsets are activated to produce the IgG PCs, we see differences between the vaccine groups for the HLA-DR + , but not the HLA-DR -PCs. This indicates that the HLA-DR + population is enriched for vaccine specificity, allowing us to detect these differences, but the HLA-DRpopulation is not.…”

Section: Discussionmentioning

confidence: 99%

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BCR repertoire sequencing: different patterns of B‐cell activation after two Meningococcal vaccines

et al. 2015

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Next-generation sequencing was used to investigate the B cell receptor heavy chain transcript repertoire of different B cell subsets (naïve, marginal zone, IgM memory and IgG memory) at baseline, and of plasma cells 7 days following administration of serogroup ACWY meningococcal polysaccharide and protein-polysaccharide conjugate vaccines. Baseline B cell subsets could be distinguished from each other using a small number of repertoire properties (clonality, mutation from germ-line and complementarity-determining region 3 length) that were conserved between individuals. However, analyzing the complementarity-determining region 3 amino acid sequence (which is particularly important for antigen binding) of the baseline subsets showed few sequences shared between individuals. In contrast, day 7 plasma cells demonstrated nearly tenfold greater sequence sharing between individuals than the baseline subsets, consistent with the plasma cells being induced by the vaccine antigen, and sharing specificity for a more limited range of epitopes. By annotating plasma cell sequences based on IgG subclass usage and mutation, and also comparing them to the sequences of the baseline cell subsets, we were able to identify different signatures after the polysaccharide and conjugate vaccines. Plasma cells produced after conjugate vaccination were predominantly IgG1, and most related to IgG memory cells. In contrast, after polysaccharide vaccination, the plasma cells were predominantly IgG2, less mutated, and were equally likely to be related to marginal zone, IgM memory or IgG memory cells. High-throughput B cell repertoire sequencing thus provides a unique insight into patterns of B cell activation not possible from more conventional measures of immunogenicity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

BCR repertoire sequencing: different patterns of B‐cell activation after two Meningococcal vaccines

et al. 2015

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“…Polyclonal stimulation of memory B cells, which causes their proliferation and differentiation into plasma cells, helps to maintain serological memory throughout life [45]. Polyclonal B cell activation by capsular polysaccharides of Neisseria meningitidis was previously reported by Oliveira et al (1996) [46].…”

Section: Discussionmentioning

confidence: 95%

The effect of pneumococcal immunization on total and antigen-specific B cells in patients with severe chronic kidney disease

2019

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BackgroundWhile the 23-valent pneumococcal polysaccharide vaccine (PPV23) is routinely used in Canada and some other countries to prevent pneumococcal infection in adults with chronic kidney disease (CKD), patients develop a suboptimal antibody response to PPV23 due to their immune dysfunction. The 13-valent pneumococcal conjugate vaccine (PCV13) has superior immunogenicity in some categories of immunocompromised adults; however, its effect on the immune response in CKD patients has only been addressed by two recent studies with conflicting results. The effect of PPV23 or PCV13 on B cells in these patients has not been previously studied. We studied the absolute numbers and proportions of B cells and subpopulations in two groups of adult patients with severe CKD pre- and 7 days post-immunization with PCV13: pneumococcal vaccine naïve and previously immunized with PPV23 (over one year ago).ResultsPPV23 immunized patients had significantly lower proportions and absolute numbers of class switched memory (CD19 + CD27 + IgM-), as well as lower absolute numbers of IgM memory (CD19 + CD27 + IgM+) and class switched B cells (CD19 + CD27-IgM-) compared to PPV23 naïve patients. Following PCV13 immunization, the differences in absolute numbers of B-cell subpopulations between groups remained significant. The PPV23 immunized group had higher proportions of CD5- B cells along with lower proportions and absolute numbers of CD5+ B cells compared to PPV23 naïve patients both pre- and post-immunization with PCV13. However, previous PPV23 immunization did not have a noticeable effect on the numbers of total IgG or serotype 6B and 14 specific antibody-secreting cells detected 7 days post-immunization with PCV13. Nevertheless, fold increase in anti-serotype 14 IgG concentrations 28 days post-PCV13 was greater in PPV23 naïve than in previously immunized patients.ConclusionsThe results suggest that immunization with PPV23 may result in long-term changes in B-cell subpopulations such as increased prevalence of CD5- B cells and decreased prevalence of class switched memory B cells in the peripheral blood. Because previous immunization with PPV23 in patients with CKD is associated with a significant decrease in the total class switched memory B cells in response to subsequent immunization with PCV13, this may reduce PCV13 immunogenicity in the setting of PPV23 followed by PCV13.Trial registrationRegistered February 24, 2015 at ClinicalTrials.gov (NCT 02370069).

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“…The antigenicity of the mucins is well established in other organisms and tissues, particularly in the case of cancer immunotherapy (for example Apostolopoulos, McKenzie & Pietersz 1996, Graham, Burchell & Taylorpapadimitriou 1996. There is evidence that cross-reactive antibodies to Neisseria meningitidis capsular polysaccharide may both react with a mucin-like molecule from trypomastigotes and cross-protect (Oliveira, Milani & Travassos 1996). In view of the importance of the question whether protection against ticks resides with oligosaccharide or protein epitopes, it is interesting that there is evidence that for the MUC-1 mucin, both may be important (Koganty, Reddish & Longenecker 1996).…”

Section: Discussionmentioning

confidence: 99%

Vaccination of cattle against the Boophilus microplus using a mucin‐like membrane glycoprotein

McKenna

Riding

Jarmey

et al. 1998

Parasite Immunology

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An antigen, BMA7, which induced partial immunity against tick infestation has been isolated from Boophilus microplus using two different protein fractionation protocols, accompanied by vaccination and parasite challenge trails. The antigen is a 63 kDa glycoprotein isolated from semi-engorged adult female ticks. Though significant, the induced immunity is less striking than that previously reported for antigen Bm86 from the same parasite. However, co-vaccination with Bm86 and BMA7 can enhance immunity over that seen with a commercial vaccine based on Bm86 alone. Limited peptide sequence information shows significant variation in the BMA7 protein occurs. The antigen has approximately 36 kDa of glycosylation, in both N-linked and O-linked oligosaccharides. There is evidence that both polypeptide and oligosaccharide are antigenic, but the chemical nature of the protective antigenic sites is not clear. There is little or no immunological response to the antigen during natural infestation with parasites, suggesting the antigen is 'concealed' and protective immunity dependent on artificial vaccination. The antigen has some similarities with the vertebrate mucins. It is widely distributed in tick tissues and membrane bound but its function is currently unknown.

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Polyclonal B-Cell activation byNeisseria meningitidis capsular polysaccharides elicit antibodies protective againstTrypanosoma cruzi infection in vitro

Cited by 6 publications

References 28 publications

BCR repertoire sequencing: different patterns of B‐cell activation after two Meningococcal vaccines

BCR repertoire sequencing: different patterns of B‐cell activation after two Meningococcal vaccines

The effect of pneumococcal immunization on total and antigen-specific B cells in patients with severe chronic kidney disease

Vaccination of cattle against the Boophilus microplus using a mucin‐like membrane glycoprotein

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