Polycomb-mediated repression of paternal chromosomes maintains haploid dosage in diploid embryos of Marchantia

Montgomery, Sean A.; Hisanaga, Tetsuya; Wang, Nan; Axelsson, Elin; Akimcheva, Svetlana; Šrámek, Miloš; Liu, Chang; Berger, Frédéric

doi:10.1101/2022.02.04.477531

Cited by 2 publications

(2 citation statements)

References 111 publications

(159 reference statements)

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“…FISH experiments were performed according to (Montgomery et al 2022). Briefly, around 5000 nuclei in 20 μl PBS buffer were incubated at 65°C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

The plant nuclear lamina disassembles to regulate genome folding in stress conditions

Wang

Tzourtzou

et al. 2022

Preprint

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The nuclear lamina (NL) is a complex network of nuclear lamins and lamin-associated nuclear membrane proteins, which scaffold the nucleus to maintain structural integrity. InArabidopsis thaliana, Nuclear Matrix Constituent Proteins (NMCPs) are essential components of the NL and are required to maintain the structural integrity of the nucleus and specific perinuclear chromatin anchoring. At the nuclear periphery, suppressed chromatin overlapping with repetitive sequences and inactive protein-coding genes are enriched. At a chromosomal level, plant chromatin organization in interphase nuclei displays flexibilities in response to various developmental cues and environmental stimuli. Based on these observations inArabidopsis, and given the role ofAtNMCPgenes (CRWN1andCRWN4) in organizing chromatin positioning at the nuclear periphery, one can expect considerable changes in chromatin-NL interactions when the global chromatin organization patterns are being altered in plants. Here, we report the highly flexible nature of plant nuclear lamina, which disassembles substantially under various stress conditions. Particularly, under heat stress, we reveal that chromatin domains, initially tethered to the nuclear envelope, remain largely associated withCRWN1and become scattered in the inner nuclear space. Via investigating the three-dimensional chromatin contact network, we further reveal that CRWN1 proteins play a structural role in shaping the changes in genome folding under heat stress. Also, CRWN1 acts as a negative transcriptional co-regulator to modulate the shift of the plant transcriptome profile in response to heat stress.

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“…FISH experiments were performed according to (Montgomery et al 2022). Briefly, around 5000 nuclei in 20 μl PBS buffer were incubated at 65°C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

The plant nuclear lamina disassembles to regulate genome folding in stress conditions

Wang

Tzourtzou

et al. 2022

Preprint

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“…Though mechanisms that regulate parent-of-origin expression in the Arabidopsis embryo at the genome level remain to be described, studies of gene expression in the Arabidopsis endosperm have uncovered multiple epigenetic pathways regulating imprinting (Batista and Köhler, 2020). In embryos of the liverwort Marchantia polymorpha, Polycomb-mediated histone 3 lysine 27 trimethylation is widely deposited on paternal chromosomes and is responsible for transcriptional silencing of hundreds of paternal alleles during the brief sporophytic phase of this lower plant (Montgomery et al, 2022). It will be interesting to determine whether epigenetic mechanisms for imprinting in the Arabidopsis endosperm or Marchantia embryo also regulate parent-of-origin gene expression in the Arabidopsis embryo.…”

Section: Parent-of-origin Transcript Differences Between the Suspenso...mentioning

confidence: 99%

Hybridization alters maternal and paternal genome contributions to early plant embryogenesis

et al. 2022

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After fertilization, zygotic genome activation (ZGA) results in a transcriptionally competent embryo. Hybrid transcriptome experiments in Arabidopsis have concluded that the maternal and paternal genomes make equal contributions to zygotes and embryos, yet embryo defective (emb) mutants in the Columbia (Col) ecotype display early maternal effects. Here we show that hybridization of Col with Landsberg erecta (Ler) or Cape Verde Islands (Cvi) ecotypes decreases maternal effects of emb mutants. Reanalysis of Col/Ler and Col/Cvi transcriptomes confirmed equal parental contributions in Col/Cvi early embryos. By contrast, thousands of genes in Col/Ler zygotes and 1-cell embryos were biallelic in one cross and monoallelic in the reciprocal cross, with analysis of intron reads pointing to active transcription as responsible for this parent-of-origin bias. Our analysis shows that, contrary to previous conclusions, the maternal and paternal genomes in Col/Ler zygotes are activated in an asymmetric manner. The decrease in maternal effects in hybrid embryos compared to isogenic Col plus differences in genome activation between Col/Cvi and Col/Ler suggest that neither of these hybrids accurately reflects general trends of parent-of-origin regulation in Arabidopsis embryogenesis.

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Polycomb-mediated repression of paternal chromosomes maintains haploid dosage in diploid embryos of Marchantia

Cited by 2 publications

References 111 publications

The plant nuclear lamina disassembles to regulate genome folding in stress conditions

The plant nuclear lamina disassembles to regulate genome folding in stress conditions

Hybridization alters maternal and paternal genome contributions to early plant embryogenesis

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