2004
DOI: 10.1016/s1097-2765(04)00128-5
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Polycomb Silencing Blocks Transcription Initiation

Abstract: Polycomb (PcG) complexes maintain the silent state of target genes. The mechanism of silencing is not known but has been inferred to involve chromatin packaging to block the access of transcription factors. We have studied the effect of PcG silencing on the hsp26 heat shock promoter. While silencing does decrease the accessibility of some restriction enzyme sites to some extent, it does not prevent the binding of TBP, RNA polymerase, or the heat shock factor to the hsp26 promoter, as shown by chromatin immunop… Show more

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Cited by 255 publications
(182 citation statements)
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“…Protein interactions have been shown to be mediated through the SAM domain (Ph) and the RING domain (RING and Bmi1), which may also play a critical role in recruitment (Levine et al, 2002;Lavigne et al, 2004). Studies have shown that the PRC1 complex does not significantly alter nucleosomal position or access of nucleases to the nucleosomal arrays (Dellino et al, 2004;Lavigne et al, 2004). This suggests that the PRC1 complex can inhibit chromatin remodeling by blocking interaction with SWI/Snf without making the template inaccessible.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Protein interactions have been shown to be mediated through the SAM domain (Ph) and the RING domain (RING and Bmi1), which may also play a critical role in recruitment (Levine et al, 2002;Lavigne et al, 2004). Studies have shown that the PRC1 complex does not significantly alter nucleosomal position or access of nucleases to the nucleosomal arrays (Dellino et al, 2004;Lavigne et al, 2004). This suggests that the PRC1 complex can inhibit chromatin remodeling by blocking interaction with SWI/Snf without making the template inaccessible.…”
Section: Discussionmentioning
confidence: 99%
“…The first complex, which includes homologues of the Drosophila esc and E(z) genes, has histone deacetylase activity (Sewalt et al, 1998;van Lohuizen et al, 1998). The second PcG complex (dPRC-1 in Drosophila, mPRC1 in mice and hPRC-H in humans) contains homologues of the Drosophila Pc, Psc, Ph, RING and Scm genes (Franke et al, 1992;Alkema et al, 1997;Satijn et al, 1997;Shao et al, 1999) and can mediate silencing of target genes by interfering with SWI/Snf chromatin remodeling machinery, blocking transcriptional initiation and the recruitment of additional silencing activities (Shao et al, 1999;Francis et al, 2001;King et al, 2002;Levine et al, 2002;Dellino et al, 2004;Lavigne et al, 2004).…”
Section: Introductionmentioning
confidence: 99%
“…In addition, PRC1 inhibits chromatin remodeling by SWI-SNF chromatin-remodeling complexes (Shao et al, 1999;Saurin et al, 2001;Francis et al, 2004) and maintains a repressive chromatin structure. The presence of TATA-binding protein Associated Factors in PRC1 suggests that its components might prevent the transition from recruitment to the promoter to initiate transcription (Dellino et al, 2004).…”
Section: Maintenance Of Hox Expression Patternmentioning
confidence: 99%
“…H2A ubiquitylation is carried out by PRC1 (13,19), which is targeted to chromatin through the recognition of H3 Lys-27 trimethylation by chromodomain-containing PcG subunits (18,20), such as M33/ Cbx2 or Pc2/Cbx4 (21). Once bound to chromatin, PcG complexes repress transcription by one or more mechanisms, including interference with nucleosome remodeling by the Swi/Snf complex chromatin remodeling (19) and/or with transcription initiation (22).…”
mentioning
confidence: 99%