Polycyclic Aromatic Hydrocarbon-degrading Bacteria from Aviation Fuel Spill Site at Ibeno, Nigeria

John, R. C.; Essien, Joseph P.; Akpan, S. B.; Okpokwasili, G. C.

doi:10.1007/s00128-012-0598-7

Cited by 52 publications

(38 citation statements)

References 28 publications

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“…The plate was sprayed with pyrene (1000 mg/L) for the isolation of pyrene-degrading bacteria. The bacterial colonies surrounded by a clear zone were scored positive (John et al 2012). All the isolates were purified through subculturing, maintained on CFMM supplemented with pyrene and preserved as glycerol stocks (15 %, v/v).…”

Section: Enrichment and Isolation Of Strainsmentioning

confidence: 99%

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

Singh

Tiwary

2016

Bioresour. Bioprocess.

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Background: Biosurfactants (BSs) are amphipathic, surface active molecules produced by microorganisms and can reduce the surface tension and interfacial tension. The present study emphasizes the isolation and structural characterization of the BS produced by Pseudomonas otitidis P4.Results: An efficient BS producing bacterial strain isolated from the unexplored coal mining site of Chirimiri, India was identified as P. otitidis P4 based on morphological, biochemical and 16S rRNA gene sequence analysis. The surface tension of the culture medium was reduced from 71.18 to 33.4 mN/m. The surface tension and emulsification activity of the BS remained stable over a wide range of temperature, pH and salt concentrations indicating its scope of application in bioremediation, food, cosmetics, and pharmaceutical industries. Structural attributes of BS were determined by biochemical tests, thin layer chromatography (TLC), Fourier transform infrared (FTIR) and nuclear magnetic resonance ( 1 H NMR) spectroscopy analyses, which confirmed the glycolipid nature of BS. Lipid and sugar fractions were the main constituents of the extracted BS. Thermogravimetric (TG) and Differential scanning calorimetry (DSC) analyses showed the thermostable nature of BS. As determined from TGA graph, the degradation temperature of biosurfactant was found to be 280 °C while complete weight loss was observed after 450 °C. Conclusion:The BS isolated from P. otitidis P4 was identified as glycolipid and showed high emulsification activity and stability in a wide range of temperature, pH and salinity which makes it suitable for various industrial and environmental applications.

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Section: Enrichment and Isolation Of Strainsmentioning

confidence: 99%

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

Singh

Tiwary

2016

Bioresour. Bioprocess.

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“…For example, the gram-negative bacteria include Alcaligenes sp. (Deveryshetty and Phale 2010; John et al 2012), Anthrobacter sp. (Kallimanis et al 2007; Kallimanis et al 2009), Burkholderia sp.…”

Section: Introductionmentioning

confidence: 99%

“…(Feng et al 2012), Micrococcus sp. (John 2012), Ochrobacterum sp. (Ghosal et al 2010), Pseudomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Multiple degradation pathways of phenanthrene by Stenotrophomonas maltophilia C6

Gao

Seo

Wang

et al. 2013

International Biodeterioration & Biodegradation

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Stenotrophomonas maltophilia strain C6, capable of utilizing phenanthrene as a sole source of carbon and energy, was isolated from creosote-contaminated sites at Hilo, Hawaii. Twenty-two metabolites of phenanthrene, covering from dihydrodiol to protocatechuic acid, were isolated and characterized. Phenanthrene was degraded via an initial dioxygenation on 1,2-, 3,4-, and 9,10-C, where the 3,4-dioxygenation and subsequent metabolisms were most dominant. The metabolic pathways were further branched by ortho- and meta-cleavage of phenanthrenediols to produce 1-hydroxy-2-naphthoic acid, 2-hydroxy-1-naphthoic acid, and naphthalene-1,2-dicarboxylic acid. These intermediates were then transformed to naphthalene-1,2-diol. 1-Hydroxy-2-naphthoic acid was also degraded via a direct ring cleavage. Naphthalene-1,2-diol underwent primarily ortho-cleavage to produce trans-2-carboxycinnamic acid and then to form phthalic acid, 4,5-dihydroxyphthalic acid and protocatechuic acid. Accumulation of salicylic acid in prolonged incubation indicated that a limited extent of meta-cleavage of naphthalene-1, 2-diol also occurred. This is the first study of detailed phenanthrene metabolic pathways by Stenotrophomonas maltophilia.

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“…High molecular weight PAHs are much more toxic than those of low molecular weight (Neff et al 2005). PAHs are ubiquitous organic pollutants that are determined in environment due to the high thermodynamic stability of benzene, their hydrophobic nature (John et al 2012). …”

Section: Issn: 2320-5407mentioning

confidence: 99%

Polycyclic-Aromatic Hydrocarbon (Pahs) in Environment and Human Health: A Review.

Kisku¹,

Tripathi²,

Raj³

2018

IJAR

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Polycyclic Aromatic Hydrocarbon-degrading Bacteria from Aviation Fuel Spill Site at Ibeno, Nigeria

Cited by 52 publications

References 28 publications

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

Multiple degradation pathways of phenanthrene by Stenotrophomonas maltophilia C6

Polycyclic-Aromatic Hydrocarbon (Pahs) in Environment and Human Health: A Review.

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