Background: Polycystic ovary syndrome (PCOS) considered one of the foremost common endocrinological diseases, which affects women of childbearing age and their quality of life. Methods: An analytical crosssectional study was conducted among a simple random sample of 826 females whose ages of 18-years-old and above in Al-Qunfudah governorate. Data were collected through a validated, electronic self-administered questionnaire to assess their knowledge toward PCOS. Results: A total of 826 women from different areas of Al-Qunfudah governorate were recruited in this study, 47.3% of them whose ages between 18 to 24 years and 39.3% were residents of Al-Qunfudah city. A percentage of 11.9 of this study sample self-reported as being previously diagnosed of PCO, of whom 65.3% received treatment. There was a significant association between knowledge score and age (p=0.000), marital status (p=0.036), educational level (p=0.000), prior knowledge about PCOS and its symptoms (p=0.000) and self-reported PCOS diagnosis (p=0.000). Conclusions: Knowledge levels varied among participants of different socio-demographic backgrounds. Better knowledge was evident among younger and married participants, university graduates, participants with previous background regarding PCOS and its symptoms, as well as participants with self-reported PCOS diagnoses.