Polydatin alleviates non-alcoholic fatty liver disease in rats by inhibiting the expression of TNF-α and SREBP-1c

Tan

et al. 2014

Exp Biol Med (Maywood)

Self Cite

Our studies and others recently demonstrate that polydatin, a resveratrol glucoside, has antioxidative and cardioprotective effects. This study aims to investigate the direct effects of polydatin on Ang II-induced cardiac hypertrophy to explore the potential role of polydatin in cardioprotection. Our results showed that in primary cultured cardiomyocytes, polydatin blocked Ang II-induced cardiac hypertrophy in a dose-dependent manner, which were associated with reduction in the cell surface area and [(3)H]leucine incorporation, as well as attenuation of the mRNA expressions of atrial natriuretic factor and β-myosin heavy chain. Furthermore, polydatin prevented rat cardiac hypertrophy induced by Ang II infusion, as assessed by heart weight-to-body weight ratio, cross-sectional area of cardiomyocyte, and gene expression of hypertrophic markers. Further investigation demonstrated that polydatin attenuated the Ang II-induced increase in the reactive oxygen species levels and NADPH oxidase activity in vivo and in vitro. Polydatin also blocked the Ang II-stimulated increases of Nox4 and Nox2 expression in cultured cardiomyocytes and the hearts of Ang II-infused rats. Our results indicate that polydatin has the potential to protect against Ang II-mediated cardiac hypertrophy through suppression of NADPH oxidase activity and superoxide production. These observations may shed new light on the understanding of the cardioprotective effect of polydatin.

Section: Introductionmentioning

confidence: 99%

Polydatin prevents angiotensin II-induced cardiac hypertrophy and myocardial superoxide generation

Tan

et al. 2014

Exp Biol Med (Maywood)

Self Cite

“…In addition, the levels of TNF-a, MDA and 4-hexanonenal are markedly suppressed by PD in the liver of HFD-fed rats. PD also decreases the gene expression of sterol-regulatory element binding protein and its target genes involved in lipogenesis, including fatty acid synthase and stearoyl-CoA desaturase 1 in HFD-fed rats (Zhang et al, 2012b). Another in vivo test reveals that PD obviously reduces serum fasting insulin, fasting blood glucose, insulin resistance index and TNF-a level, and improves the insulin sensitivity index level, whose effects at high dose are better than those of fenofibrate (Zhang & Lv, 2010).…”

Section: Hepatoprotective Effectsmentioning

confidence: 99%

Polydatin: A review of pharmacology and pharmacokinetics

Peng

2013

Pharmaceutical Biology

217

162

Context: Polydatin, also named piceid (3,4 0 ,5-trihydroxystilbene-3-b-D-glucoside, PD), is a monocrystalline compound isolated from Polygonum cuspidatum Sieb. et Zucc. (Polygonaceae), but is also detected in grape, peanut, hop cones, red wines, hop pellets, cocoa-containing products, chocolate products and many daily diets. There are numerous investigations reported of PD in the past 22 years, but they are usually scattered across various publications, which may block further research and clinical use of PD. Objective: The article summarizes and evaluates the published scientific information of PD pharmacological effects and pharmacokinetics since 1990. Materials and methods: The information from 98 cases included in this review was compiled using major databases such as MEDLINE, Elsevier, Springer, PubMed, Scholar and CNKI. Results: Numerous pharmacological investigations of PD mainly focus on cardiovascular effects, neuroprotection, anti-inflammatory and immunoregulatory effects, anti-oxidation, anti-tumor, liver and lung protection, etc. Conclusion: A great number of pharmacological and pharmacokinetic investigations in the past 22 years have demonstrated that PD has favorable therapeutic properties, indicating its potential as an effective material. However, further research is needed to explore its molecular mechanisms of action and definitive target proteins.

Biomedical Chromatography

“…For example, chlorogenic acid could prevent fatty liver by upregulating the expression of peroxisomal proliferator‐activated receptor α (PPARα) (Alqarni, Bassiouni, Badr, & Ali, ; Wan et al, ). Geniposide and polydatin could alleviate high‐fat‐diet‐induced hepatic steatosis in rats, especially because geniposide exhibits antioxidant actions and could regulate the adipocytokine release and expression of PPARα (Ma et al, ), whereas polydatin could reduce liver tumor necrosis factor‐α (TNF‐α) expression, lipid peroxidation level, and sterol regulatory element‐binding protein‐1c (SREBP‐1c)‐mediated lipogenesis (Zhang, Tan, Yao, & Zhang, ). Resveratrol was proved to reverse the hepatic triglyceride content in high‐fructose‐corn‐syrup‐induced insulin resistance in rats by improving insulin signaling and suppressing the expression levels of SREBP‐1c in liver (Sadi et al, ).…”

Section: Resultsmentioning

confidence: 99%

Systematic investigation on the chemical basis of anti‐NAFLD Qushi Huayu Fang. Part 1: A study of metabolic profiles in vivo and in vitro by high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry

Liu

Sun

Tian

et al. 2020

Qushi Huayu Fang (QHF) is a clinic-empirical prescription for treating non-alcoholic fatty liver disease (NAFLD) in China, which is composed of five herbs. However, the bioactive constituents responsible for the efficacy of QHF remain unclear. Thus, a high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry method was established and adopted to identify the constituents of QHF, and profile its metabolism in vivo and in vitro. Among the 66 constituents in QHF, only 14 compounds of six structural types were absorbed, and 34 metabolites were generated through eight metabolic pathways. A total of 20 metabolites were first reported, including four organic acids, one iridoid, two flavones, five naphthols, three anthraquinones, and five stilbenes. Glucuronidation and sulfation were the main metabolic pathways, and the intestinal metabolism played an important role in the biotransformation of QHF. Many compounds, especially those detected in the liver, the target organ of QHF, were reported to display the anti-NAFLD activity. This is the first study to explore the constituents of QHF and its metabolism in vivo and in vitro, thus realizing the first step to clarify the chemical basis of QHF qualitatively, and laying the foundation for further research on the anti-NAFLD mechanism of QHF. K E Y W O R D S HPLC-Q-TOF, intestinal bacteria, liver, metabolism, Qushi Huayu FangAbbreviations: HPLC-Q-TOF, high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry; IRE1α, inositol-requiring transmembrane kinase/endoribonuclease 1α; NAFLD, non-alcoholic fatty liver disease; Nrf-2, nuclear erythroid 2-related factor 2; PI3K, phosphatidylinositol 3-kinase; PPARα, peroxisomal proliferator-activated receptor α; QHF, Qushi Huayu Fang; SD, Sprague Dawley; SREBP-1c, sterol regulatory element-binding protein-1c; TCM, traditional Chinese medicines; TNF-α, tumor necrosis factor-α; XBP1s, X-box binding protein 1; YCHD, Yinchenhao Decoction. # Huan Liu and Zhaolin Sun contributed equally to this study.