Polydatin Attenuates 14.1 MeV Neutron-Induced Injuries via Regulating the Apoptosis and Antioxidative Pathways and Improving the Hematopoiesis of Mice

Abstract

With more powerful penetrability and ionizing capability, high energetic neutron radiation (HENR) often poses greater threats than photon radiation, especially on such occasions as nuclear bomb exposure, nuclear accidents, aerospace conduction, and neutron-based radiotherapy. Therefore, there emerges an urgent unmet demand in exploring highly efficient radioprotectants against HENR. In the present study, high-throughput 14.1 MeV neutrons were generated by the high-intensity D-T fusion neutron generator (HINEG) and succeeded in establishing the acute radiation syndrome (ARS) mouse model induced by HENR. A series of preclinical studies, including morphopathological assessment, flow cytometry, peripheral complete blood, and bone marrow karyocyte counting, were applied showing much more serious detriments of HENR than the photon radiation. In specific, it was indicated that surviving fraction of polydatin- (PD-) treated mice could appreciably increase to up to 100% when they were exposed to HENR. Moreover, polydatin contributed much in alleviating the HENR-induced mouse body weight loss, spleen and testis indexes decrease, and the microstructure alterations of both the spleen and the bone marrow. Furthermore, we found that the HENR-damaged hematopoiesis was greatly prevented by PD treatment in such aspects as bone marrow hemocytogenesis, splenocytes balancing, or even the peripheral blood cellularity. The additional IHC investigations revealed that PD could exert potent hematopoiesis-promoting effects against HENR via suppressing apoptosis and promoting the antioxidative enzymes such as HO-1.

Polydatin Radiosensitizes Lung Cancer while Preventing Radiation Injuries by Modulating Tumor-infiltrating B Cells

“…According to the earlier research, PD can considerably boost the body's ability to tolerate radiation harm, as evidenced again by the current observations of animal survival [15,20]. The mechanism of its radiation protection action, as a natural plant extract that has been thoroughly explored, could include scavenging free radicals, activating antioxidant pathways, modulating immunity, boosting hematopoiesis, vascular endothelial protection, and so on [6,15,21,22]. In recent years, the study of its exact mechanism has been a hot topic in the field of radiation protection research.…”

Molecular Hydrogen Reduces Electromagnetic Pulse-Induced Male Rat Reproductive System Damage in a Rodent Model

“…After anesthesia, two testicles from both sides of each animal were dissected, weighed, and fixed or frozen with liquid nitrogen as soon. Subsequently, the testicular index was calculated using the following formula [ 27 ]: …”

Old dog, new tricks: Polydatin as a multitarget agent for current diseases

“…Radiation affects haematopoietic function. PD improved high‐energy neutron radiation‐induced haematopoietic injury by inhibiting cell apoptosis and promoting antioxidant enzymes, such as HO‐1 (Guo et al, 2020).…”