Polydatin Encapsulated Poly [Lactic-co-glycolic acid] Nanoformulation Counteract the 7,12-Dimethylbenz[a] Anthracene Mediated Experimental Carcinogenesis through the Inhibition of Cell Proliferation

Abstract

In the present study, the authors have attempted to fabricate Polydatin encapsulated Poly [lactic-co-glycolic acid] (POL-PLGA-NPs) to counteract 7,12-dimethyl benzyl anthracene (DMBA) promoted buccal pouch carcinogenesis in experimental animals. The bio-formulated POL-PLGA-NPs were characterized by dynamic light scattering (DLS), Fourier transform infrared (FTIR) spectroscopy, X-ray powder diffraction (XRD) pattern analysis, and transmission electron microscope (TEM). In addition, the nano-chemopreventive potential of POL-PLGA-NPs was assessed by scrutinizing the neoplastic incidence and analyzing the status of lipid peroxidation, antioxidants, phase I, phase II detoxification status, and histopathological changes and in DMBA-treated animals. In golden Syrian hamsters, oral squamous cell carcinoma (OSCC) was generated by painting with 0.5% DMBA in liquid paraffin three times a week for 14 weeks. After 100% tumor formation was observed, high tumor volume, tumor burden, and altered levels of biochemical status were observed in the DMBA-painted hamsters. Intra-gastric administration of varying concentration of POL-PLGA-NPs (7.5, 15, and 30 mg/kg b.wt) to DMBA-treated hamsters assumedly prevents oncological incidences and restores the status of the biochemical markers. It also significantly enhances the apoptotic associated and inhibits the cancer cell proliferative markers expression (p53, Bax, Bcl-2, cleaved caspase 3, cyclin-D1). The present study reveals that POL-PLGA-NPs is a penitential candidate for nano-chemopreventive, anti-lipid peroxidative, and antioxidant potential, and also has a modulating effect on the phase I and Phase II detoxification system, which is associated with reduced cell proliferation and induced apoptosis in experimental oral carcinogenesis.

Insights on the Atmospheric-Pressure Plasma-Induced Free-Radical Polymerization of Allyl Ether Cyclic Carbonate Liquid Layers

“…Figure 2 shows the recorded FTIR spectra, normalized according to the intensity of the absorption band at 1755 cm −1 (C=O stretching carbonate ring), of samples exposed to plasma for 30, 40, 50, 70 and 90 runs. Increasing the exposition time not only led to the decrease in and disappearance of the bands attributed at the allyl groups ( Figure 2 b), but the formation of alkyl backbone could be detected by the appearance of and increase in the plasma exposition time of the absorption bands in the range of 3050–2800 cm −1 , corresponding to C-H stretching, and at 760 cm −1 , related to the rocking vibrations of CH 2 of the long alkyl chains [ 8 , 12 , 29 , 30 ].…”

Ameliorative effect of polydatin and polydatin-loaded chitosan nanoparticles against diabetes-induced pulmonary disorders in rats

“…The current investigation elucidated that oral treatment of PD-CSNPs, PD and MET reduced lipid peroxidation products and increased the enzymatic antioxidant activity; also exhibited their anti-inflammatory effects and suppressed inflammatory damage in diabetic rats through enhancing expression of Nrf2 and HO-1, which reduces oxidative stress. Notably, polydatin nanoparticles revealed anti-lipid peroxidation and antioxidant activity [45]. Also, PD reduced hyperosmolar stress-induced inflammation by attenuating NF-κβ translocation to the nucleus and the TNF-α mRNA expression [16].…”

Polydatin, a Glycoside of Resveratrol, Induces Apoptosis and Inhibits Metastasis Oral Squamous Cell Carcinoma Cells In Vitro

“…Also, a study to increase cell membrane permeability by encapsulating polydatin was published. They found that polydatin and metformin encapsulated with Poly[lactic-co-glycolic acid] (POL-PLGA-NPs) were to induce a mitochondrial-mediated apoptotic mechanism in vivo [ 39 ]. Therefore, in our results, the reason that a rather high concentration of polydatin induced apoptosis and inhibited metastasis could be inferred that polydatin was not properly absorbed during penetration into the cell membrane.…”