Polydatin protects against acute cholestatic liver injury in mice via the inhibition of oxidative stress and endoplasmic reticulum stress

Fang, Jinan; Luo, Lingling; Ke, Zunli; Liu, Chuhe; Yin, Li; Yao, Yin; Huang, Cheng; Zheng, Peiyong

doi:10.1016/j.jff.2019.02.029

Cited by 15 publications

(9 citation statements)

References 55 publications

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“…In agreement with previous study [ 33 ], it seems that the increased level of total bilirubin in the BDL group is due to liver cell destruction and bile duct obstruction caused by BDL. In addition, the increase in total protein content was observed in the present study to be a self-healing mechanism in the process of liver regeneration during the response to the acute phase of BDL stress [ 34 ]. Traditional Chinese medicine has used Portulaca oleracea , also known as Ma-Chi-Xian, to resolve toxin and clear heat.…”

Section: Discussionmentioning

confidence: 85%

Portulaca oleracea methanolic extract attenuate bile duct ligation-induced acute liver injury through hepatoprotective and anti-inflammatory effects

Moslemi

Bahrami

Hosseini

et al. 2021

Heliyon

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Introduction: Cholestasis is a liver disease caused by a malfunction of the hepato-biliary system. Oxidative stress as a systemic complication is the main characteristic of cholestasis. The aim of this study was to evaluate the antiinflammatory and hepatoprotective effects of Portulaca oleracea (PO) methanolic extract on liver dysfunction and tissue damage induced by bile duct ligation (BDL) in rats. Materials and methods: Twenty-eight male Wistar rats were randomly divided into four groups: sham control (SC), BDL alone, SC plus 500 mg/kg methanolic extract of PO orally for 1 week, and BDL plus 500 mg/kg methanolic extract of PO orally for 1 week. After 1 week, the animals were anesthetized, and the liver and blood samples were taken from each animal. Biochemical parameters, oxidative stress biomarkers, histopathological changes, as well as the gene expression of IL-1, TNF-α, TGF-β, and α-SMA have been evaluated. Results:The methanolic extract of PO at a dose of 500 mg/kg significantly decreased the plasma levels of aminotransferases, alkaline phosphatase as compared to BDL group (P < 0.05), while it had no significant effect on the levels of oxidative stress markers in the hepatic tissue. The plasma level of malondialdehyde and ferricreducing antioxidant power were markedly elevated in the BDL group in comparison to SC group (P < 0.05), while treatment with PO significantly reduced these markers (P < 0.05). The administration of PO attenuated hydroxyproline content, bile duct proliferation, and inflammation score in the cholestatic liver in contrast to nontreated BDL rats (P < 0.05). Moreover, the methanolic extract of PO markedly declined the expression of TNF-α and TGF-β pro inflammatory genes in contrast to BDL rats. Conclusions: Taken together, our findings showed that PO attenuated liver injury by decreasing liver function tests, inflammation, and hydroxyproline content. As a result, it is suggested that PO can be applied in cholestatic liver damage as a therapeutic or adjuvant agent.

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Section: Discussionmentioning

confidence: 85%

Portulaca oleracea methanolic extract attenuate bile duct ligation-induced acute liver injury through hepatoprotective and anti-inflammatory effects

Moslemi

Bahrami

Hosseini

et al. 2021

Heliyon

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“…According to the study of Zhao et al, MDA levels increased in both serum and liver tissue in the BDL group [ 27 ]. Fang et al indicated a significant increase in MDA level in BDL-induced cholestasis rats compared with the SC group [ 31 ]. The present data showed that the plasma MDA of cholestatic rats significantly increased compared to the SC-V group, and OM had no effect on plasma MDA levels.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that the administration of medicinal plants and natural compounds with antioxidant activity such as hydroalcoholic extract of Watercress [ 20 ], Ziziphus mauritiana leaf [ 35 ], polydatin [ 31 ], quercetin [ 36 ], and Marjoram oil [ 37 ] can protect the liver against oxidative damage caused by cholestasis. SOD and CAT are antioxidants that prevent the production of free radicals and also work as part of the first line of defense in the living cells [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and Anti-Inflammatory Effects of Origanum majorana L. Methanolic Extract on Bile Duct Ligation in Male Rats

Gheitasi

Motaghi

Sadeghi

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Introduction. Cholestasis is caused by malfunction of the hepatobiliary system. This disorder is the result of the accumulation of bile fatty acids and other toxins in the liver. The aim of the current study was to investigate the antioxidative and hepatoprotective effects of methanolic extract of Origanum majorana L. (OM) on hepatic disorder and tissue damage induced by bile duct ligation (BDL) in rats. Materials and methods. Twenty-eight male Wistar rats were randomly divided into 4 groups including sham control group received vehicle (SC-V), bile duct ligation received vehicle (BDL-V), bile duct ligation group received OM extract (BDL + OM), and sham control group received OM extract (SC + OM). One day after surgery, the animals received vehicle or methanolic extract of OM 300 mg/kg/day for 7 consecutive days by oral gavage. Finally, the animals were anesthetized and the blood samples were collected from each animal. After sacrificing of animals, liver tissue from each rat was removed and divided into three parts: one part was used for preparing of homogenized tissue, one part was fixed in 10% neutral formalin for histopathology examination, and the third part was kept in liquid nitrogen for gene expression analysis. Biomarkers of oxidative stress in the liver tissue and serum, as well as histopathological changes of the liver, were assessed. Also, the gene expression of IL-1, TNF-α, TGF-β, and α-SMA has been measured. Results. The results showed that BDL-V significantly increased the activity of ALT, AST, ALP, and total bilirubin compared to the SC-V group. The oxidative stress markers such as MDA and FRAP significantly increased due to BDL, while the CAT activity reduced in the BDL-V group compared to SC-V group. Oral treatment with OM reduced ALT and AST activity, although it was not statistically significant. OM treatment considerably increased the activity of CAT compared to BDL group. BDL-V induced a significant histological change in the liver, while treatment with OM at a dose of 300 mg/kg showed a minor effect on histopathological changes. In addition, the mRNA of IL-1, TNF-α, TGF-β, and α-SMA significantly increased in the BDL-V group, while treatment with OM only significantly reduced TGF-β in comparison with BDL-V rats. Conclusions. The results of the present study showed that oral administration of OM extract had a moderate protective effect on cholestasis due to BDL. Indeed, more studies with different doses of extract are needed to confirm this finding.

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“…PD could improve SOD activity, reduce MDA and serum AST, ALT, ALP, total bile acid (TBA), and total bilirubin (TBIL) levels, and inhibit ER stress, p-elf2 α , CHOP, and hepatocellular apoptosis in the cholestatic mice induced by alpha-naphthylisothiocyanate (ANIT) and bile duct ligation (BDL). The results suggest that PD may alleviate cholestatic liver damage by inhibition of oxidative stress, ER stress, and apoptosis [ 81 ].…”

Section: Effects Of Polydatin On Liver Diseases Via Regulating Oxidat...mentioning

confidence: 99%

Polydatin: A Critical Promising Natural Agent for Liver Protection via Antioxidative Stress

Tang

Zhang

Duan

et al. 2022

Oxidative Medicine and Cellular Longevity

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Polydatin, one of the natural active small molecules, was commonly applied in protecting and treating liver disorders in preclinical studies. Oxidative stress plays vital roles in liver injury caused by various factors, such as alcohol, viral infections, dietary components, drugs, and other chemical reagents. It is reported that oxidative stress might be one of the main reasons in the progressive development of alcohol liver diseases (ALDs), nonalcoholic liver diseases (NAFLDs), liver injury, fibrosis, hepatic failure (HF), and hepatocellular carcinoma (HCC). In this paper, we comprehensively summarized the pharmacological effects and potential molecular mechanisms of polydatin for protecting and treating liver disorders via regulation of oxidative stress. According to the previous studies, polydatin is a versatile natural compound and exerts significantly protective and curative effects on oxidative stress-associated liver diseases via various molecular mechanisms, including amelioration of liver function and insulin resistance, inhibition of proinflammatory cytokines, lipid accumulation, endoplasmic reticulum stress and autophagy, regulation of PI3K/Akt/mTOR, and activation of hepatic stellate cells (HSCs), as well as increase of antioxidant enzymes (such as catalase (CAT), glutathione peroxidase (GPx), glutathione (GSH), superoxide dismutase (SOD), glutathione reductase (GR), and heme oxygenase-1 (HO-1)). In addition, polydatin acts as a free radical scavenger against reactive oxygen species (ROS) by its phenolic and ethylenic bond structure. However, further clinical investigations are still needed to explore the comprehensive molecular mechanisms and confirm the clinical treatment effect of polydatin in liver diseases related to regulation of oxidative stress.

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Polydatin protects against acute cholestatic liver injury in mice via the inhibition of oxidative stress and endoplasmic reticulum stress

Cited by 15 publications

References 55 publications

Portulaca oleracea methanolic extract attenuate bile duct ligation-induced acute liver injury through hepatoprotective and anti-inflammatory effects

Portulaca oleracea methanolic extract attenuate bile duct ligation-induced acute liver injury through hepatoprotective and anti-inflammatory effects

Antioxidant and Anti-Inflammatory Effects of Origanum majorana L. Methanolic Extract on Bile Duct Ligation in Male Rats

Polydatin: A Critical Promising Natural Agent for Liver Protection via Antioxidative Stress

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