Polydopamine‐collagen complex to enhance the biocompatibility of polydimethylsiloxane substrates for sustaining long‐term culture of L929 fibroblasts and tendon stem cells

Abstract

Polydimethylsiloxane (PDMS) is a commercialized polymer extensively used in the fabrication of versatile microfluidic microdevices for studies in cell biology and tissue engineering. However, the inherent surface hydrophobicity of PDMS is not optimal for cell culture and thus restrains its applications for investigation of long-term behaviors of fibroblasts and stem cells. To improve the surface biocompatibility of PDMS, a facile technique was developed by modifying the PDMS surface with polydopamine-collagen (COL/PDA) complex. The successful synthesis of COL/PDA was verified through proton nuclear magnetic resonance spectroscopy. Compared to surface coating solely with COL or PDA, the surface wettability was significantly improved on COL/PDA-modified PDMS substrates based on water contact angle characterizations. The modified PDMS surface remarkably enhanced the initial adhesion and long-term proliferation of L929 fibroblasts and tendon stem cells (TSCs). Additionally, the effects of COL/PDA coating on cell viability and apoptosis were further investigated under prolonged incubation. We found that the COL/PDA coating on PDMS resulted in a substantial increase of cell viability compared to native PDMS, and the cell apoptosis was considerably impeded on the modified PDMS. This study demonstrated that COL/PDA coating can effectively enhance the surface biocompatibility of PDMS as verified by the enhanced adhesion and long-term proliferation of L929 fibroblasts and TSCs. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 408-418, 2018.

Simple Surface Modification of Poly(dimethylsiloxane) via Surface Segregating Smart Polymers for Biomicrofluidics

“…This may lead to poor device performance even if surface hydrophilicity is enhanced. To date, there are some studies that evaluated the biocompatibility or cell adhesion of modified PDMS microfluidic devices or slabs using mammalian A549 cells 43 , L929 mouse fibroblasts 44 , tendon stem cells 45 , mesenchymal stem cells (MSCs) 46 , brain cerebral cortex cells 47 , HeLa cells 48 , and stroma cells 49 . To our knowledge, none of the previous PDMS modification strategies were evaluated for compatibility with hepatocytes, the parenchymal cells of the liver, which are highly susceptible to adverse reactions.…”

Polydopamine-assisted surface modification for orthopaedic implants

“…They demonstrated that PDA coating could enhance chondrocyte adhesion to a series of biodegradable polymers. PDA-based surface modification has also been applied in ligament and tendon repair [77], [78]. For example, PDA was used to gradually immobilize platelet-derived growth factor-BB on nanofibres to control the differentiation of adipose-derived stem cells into tenocytes in a spatially controlled manner.…”

Enhanced Biocompatibility and Differentiation Capacity of Mesenchymal Stem Cells on Poly(dimethylsiloxane) by Topographically Patterned Dopamine

“…However, PDMS is inadequate for protein adsorption and cell adhesion because of its difficulty to anchor bioactive ligands. 36 Literature reported that the inherent hydrophobicity of PDMS was the main cause unfavorable for maintaining cell culture stably. 4,37 The modification of the hydrophobic surfaces by PDA was developed on PDMS templates with tunable micropattern imprinting to various substrates.…”