Polydopamine-Laced Biomimetic Material Stimulation of Bone Marrow Derived Mesenchymal Stem Cells to Promote Osteogenic Effects

Lee, Dong Joon; Lee, Yan Ting; Zou, Rui; Daniel, Renie; Ko, Ching Chang

doi:10.1038/s41598-017-13326-y

Cited by 33 publications

(30 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a previous study, HCCS‐PDA in particle form was tested for bone regeneration capacity both in vitro and in vivo to observe sole effect of HCCS‐PDA material. The results showed that the HCCS‐PDA enhanced local bone formation in calvarial CSD model, forming tight interface between HCCS‐PDA particle and newly formed bone . With well‐designed scaffold, the amount of bone regeneration is expected to increase.…”

Section: Introductionmentioning

confidence: 98%

“…The results showed that the HCCS-PDA enhanced local bone formation in calvarial CSD model, forming tight interface between HCCS-PDA particle and newly formed bone. 2 With well-designed scaffold, the amount of bone regeneration is expected to increase. Computeraided design (CAD) modelling has enabled precise control of the scaffold architecture, including the pore size, connectivity, dimensions and shape.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Effect of pore size in bone regeneration using polydopamine‐laced hydroxyapatite collagen calcium silicate scaffolds fabricated by 3D mould printing technology

Lee

Kwon

Kim

et al. 2019

Orthod Craniofacial Res

Self Cite

View full text Add to dashboard Cite

Structured Abstract Objective The pore size of the scaffold is a critical factor in repairing large bone defect. Here, we investigated the potential of bone regeneration using novel nanocomposite polydopamine‐laced hydroxyapatite collagen calcium silicate (HCCS‐PDA) scaffolds with two different pore sizes, 250 and 500 μm. Samples/Setting A total of 12 male Sprague‐Dawley rats were implanted with HCCS‐PDA scaffold with pore size of either 250 or 500 μm into surgically created critical‐sized defect (CSD). Methods HCCS‐PDA scaffolds were fabricated using mould printing technique. The effect of pore size on mechanical strength of the scaffolds was assessed by compression testing. After seeding with rat mesenchymal stem cells (rMSCs), the scaffolds were implanted, and new bone formation was evaluated using microCT and histomorphometric analysis after 8 weeks. Results MicroCT and histology analysis demonstrated restricted peripheral new bone formation in either dural or periosteal side and limited new bone formation in the 250 μm pore scaffold. Conversely, the 500‐μm pore scaffold showed more penetration of new bone into the scaffold and greater bone regeneration in the rat CSD. Conclusion Based on our results, which demonstrated improved new bone formation in 500 μm pores scaffold, we can conclude that effective scaffold pore size that induces osteointegration and bone regeneration is around 500 μm for HCCS‐PDA nanocomposite scaffold.

show abstract

Section: Introductionmentioning

confidence: 98%

mentioning

confidence: 99%

Effect of pore size in bone regeneration using polydopamine‐laced hydroxyapatite collagen calcium silicate scaffolds fabricated by 3D mould printing technology

Lee

Kwon

Kim

et al. 2019

Orthod Craniofacial Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Bliziotes et al (2000) showed that the deletion of the DA transporter gene in mice reduces the cancellous bone mass. Moreover, DA promotes osteoblastic differentiation of murine preosteoblasts (Lee et al, 2015) and rat bone marrow mesenchymal stem cells (MSCs; Lee, Lee, Zou, Daniel, & Ko, 2017).…”

mentioning

confidence: 99%

Expression and function of dopamine in odontoblasts

Fujino

Tomokiyo

Itoyama

et al. 2019

Journal Cellular Physiology

View full text Add to dashboard Cite

Dopamine (DA) is produced from tyrosine by tyrosine hydroxylase (TH). A recent study has reported that DA promotes the mineralization of murine preosteoblasts.However, the role of DA in odontoblasts has not been examined. Therefore, in this investigation, we researched the expression of TH and DA in odontoblasts and the effects of DA on the differentiation of preodontoblasts (KN-3 cells). Immunostaining showed that TH and DA were intensely expressed in odontoblasts and preodontoblasts of rat incisors and molars. KN-3 cells expressed D1-like and D2-like receptors for DA. Furthermore, DA promoted odontoblastic differentiation of KN-3 cells, whereas an antagonist of D1-like receptors and a PKA signaling blocker, inhibited such differentiation. However, antagonists of D2-like receptors promoted differentiation. These results suggested that DA in preodontoblasts and odontoblasts might promote odontoblastic differentiation through D1-like receptors, but not D2like receptors, and PKA signaling in an autocrine or paracrine manner and plays roles in dentinogenesis. K E Y W O R D S differentiation, dopamine, odontoblast, tyrosine hydroxylase

show abstract

“…From our Prestoblue ® assay and cytoskeleton results, it was observed that PDA coating significantly up-regulates WJMSCs proliferation. Even though we are still unsure of the exact mechanism behind this explanation, it can be hypothesized that the ions dissolved and released from our PDA-coated cements were able to positively modulate cell behaviors through the synergistic combinations between the functional groups present in PDA, and the presence of ionic components on the coating surfaces [ 47 , 48 ]. Moreover, we believed that some WJMSC had begun to enter the stage of osteogenesis differentiation, so the growth rate of the cells would gradually slow.…”

Section: Resultsmentioning

confidence: 99%

Surface Modification of Calcium Silicate via Mussel-Inspired Polydopamine and Effective Adsorption of Extracellular Matrix to Promote Osteogenesis Differentiation for Bone Tissue Engineering

Kao

Chen

et al. 2018

Materials

View full text Add to dashboard Cite

Calcium silicate-based cement has garnered huge interest in recent years, due to its versatility and potential in mass fabrication of a variety of bioceramics. For this study, the main objective was to fabricate functionalized calcium silicate (CS) powder integrated with a simple bio-inspired surface modification using polydopamine (PDA), to regulate cellular behaviors such as cellular adhesion, and subsequently cell differentiation and proliferation. For this study, scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) techniques were used to analyze the chemical compositions and observe the surface characteristics of our PDA coated CS cements. Such modifications were found to enhance Wharton Jelly’s mesenchymal stem cells (WJMSC) in various ways. Firstly, PDA-coated CS cements were found to significantly enhance cell adhesion with higher expressions of cell adhesion markers, such as focal adhesion kinase and integrins. This was further supported by morphology analysis of the cells. This enhanced cell adhesion, in turn, led to significantly higher secretion of extracellular matrix (ECM) proteins, such as collagen I and fibronectin, which directly promoted cell attachments and proliferation. In our osteogenesis assays, it was found that secretion and expression of osteogenesis related genes and proteins were significantly higher and were dependent on the PDA content. Therefore, these results demonstrated that such simple bio-inspired modification techniques of synthetic degradable CS cements can be applied as a future modification, to modify and convert inert surfaces of synthetic bone grafts to enhance and modulate the cell behaviors of WJMSCs. This in turn can be used as a potential alternative for further bioengineering research.

show abstract

Polydopamine-Laced Biomimetic Material Stimulation of Bone Marrow Derived Mesenchymal Stem Cells to Promote Osteogenic Effects

Cited by 33 publications

References 33 publications

Effect of pore size in bone regeneration using polydopamine‐laced hydroxyapatite collagen calcium silicate scaffolds fabricated by 3D mould printing technology

Effect of pore size in bone regeneration using polydopamine‐laced hydroxyapatite collagen calcium silicate scaffolds fabricated by 3D mould printing technology

Expression and function of dopamine in odontoblasts

Surface Modification of Calcium Silicate via Mussel-Inspired Polydopamine and Effective Adsorption of Extracellular Matrix to Promote Osteogenesis Differentiation for Bone Tissue Engineering

Contact Info

Product

Resources

About