Polydopamine-modified decellularized intestinal scaffolds loaded with adipose-derived stem cells promote intestinal regeneration

Wan, Jian; Wu, Tianqi; Wang, Keyi; Xia, Kai; Yin, Lu; Chen, Chunqiu

doi:10.1039/d2tb01389d

Cited by 5 publications

(3 citation statements)

References 65 publications

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“…This study demonstrated that PDA coating significantly enhanced the roughness, hydrophilicity, and protein absorption capacity of the film, which promoted the adhesion and migration of MSCs onto biomaterial surfaces. In another investigation conducted by Wan et al [ 170 ], a PDA-mediated surface modification of decellularized intestinal scaffolds was combined with ADSC to promote intestinal wound healing. The PDA-coated scaffold effectively supported the growth and proliferation of ADSCs and enhanced their secretory activity for paracrine effects.…”

Section: Interaction Between Scaffolds and Mscsmentioning

confidence: 99%

Biological scaffold as potential platforms for stem cells: Current development and applications in wound healing

Xiang,

Kang,

et al. 2024

World J Stem Cells

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Wound repair is a complex challenge for both clinical practitioners and researchers. Conventional approaches for wound repair have several limitations. Stem cell-based therapy has emerged as a novel strategy to address this issue, exhibiting significant potential for enhancing wound healing rates, improving wound quality, and promoting skin regeneration. However, the use of stem cells in skin regeneration presents several challenges. Recently, stem cells and biomaterials have been identified as crucial components of the wound-healing process. Combination therapy involving the development of biocompatible scaffolds, accompanying cells, multiple biological factors, and structures resembling the natural extracellular matrix (ECM) has gained considerable attention. Biological scaffolds encompass a range of biomaterials that serve as platforms for seeding stem cells, providing them with an environment conducive to growth, similar to that of the ECM. These scaffolds facilitate the delivery and application of stem cells for tissue regeneration and wound healing. This article provides a comprehensive review of the current developments and applications of biological scaffolds for stem cells in wound healing, emphasizing their capacity to facilitate stem cell adhesion, proliferation, differentiation, and paracrine functions. Additionally, we identify the pivotal characteristics of the scaffolds that contribute to enhanced cellular activity.

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Section: Interaction Between Scaffolds and Mscsmentioning

confidence: 99%

Biological scaffold as potential platforms for stem cells: Current development and applications in wound healing

Xiang,

Kang,

et al. 2024

World J Stem Cells

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“…It has recently been shown that using the lentiviral system (vectors expressing Pdx1 and MafA/NeuroD1) it is possible to differentiate them into insulin-producing cells [ 108 ]. Using animal models, it has been shown that AD-MSC cells administered on appropriate decellularized intestinal scaffolds (polydopamine (PDA)-mediated surface modification) significantly accelerate intestinal regeneration, stimulate angiogenesis, and inhibit inflammation [ 109 ]. In another study, cells were transfected using liposomes with the plasmid Netrin-1.…”

Section: Bioengineered Ad-mscs In Regenerative Medicinementioning

confidence: 99%

Adipose-Derived Mesenchymal Stromal Cells in Basic Research and Clinical Applications

Czerwiec

Zawrzykraj

Deptuła

et al. 2023

IJMS

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Adipose-derived mesenchymal stromal cells (AD-MSCs) have been extensively studied in recent years. Their attractiveness is due to the ease of obtaining clinical material (fat tissue, lipoaspirate) and the relatively large number of AD-MSCs present in adipose tissue. In addition, AD-MSCs possess a high regenerative potential and immunomodulatory activities. Therefore, AD-MSCs have great potential in stem cell-based therapies in wound healing as well as in orthopedic, cardiovascular, or autoimmune diseases. There are many ongoing clinical trials on AD-MSC and in many cases their effectiveness has been proven. In this article, we present current knowledge about AD-MSCs based on our experience and other authors. We also demonstrate the application of AD-MSCs in selected pre-clinical models and clinical studies. Adipose-derived stromal cells can also be the pillar of the next generation of stem cells that will be chemically or genetically modified. Despite much research on these cells, there are still important and interesting areas to explore.

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“…It has been approved by the FDA, and the xenogenically derived SIS has been widely used in various tissue repair studies. Furthermore, in vivo has indicated that it is nonimmunogenic. − Several studies have verified that SIS can provide a good microenvironment to facilitate the proliferation, attachment, and osteogenic differentiation of fibroblast bone, osteoblasts, and marrow mesenchymal stem cells. ,− In our previous study, SIS was applied to modify commercially available PMMA cement to optimize the biological performance and in vivo repair effect of the basic material . Though PMMA with 20 or 40% SIS powder showed excellent bioactivity to enhance the new bone formation, the compressive strength of the constructs was hard to meet the requirement of clinical application (ISO5833), which was essential for clinical application.…”

Section: Introductionmentioning

confidence: 99%

Preclinical Evaluation of Bioactive Small Intestinal Submucosa-PMMA Bone Cement for Vertebral Augmentation

Zhang,

Cai,

et al. 2024

ACS Biomater. Sci. Eng.

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In vertebroplasty and kyphoplasty, bioinert poly(methyl methacrylate) (PMMA) bone cement is a conventional filler employed for quick stabilization of osteoporotic vertebral compression fractures (OVCFs). However, because of the poor osteointegration, excessive stiffness, and high curing temperature of PMMA, the implant loosens, the adjacent vertebrae refracture, and thermal necrosis of the surrounding tissue occurs frequently. This investigation addressed these issues by incorporating the small intestinal submucosa (SIS) into PMMA (SIS-PMMA). In vitro analyses revealed that this new SIS-PMMA bone cement had improved porous structure, as well as reduced compressive modulus and polymerization temperature compared with the original PMMA. Furthermore, the handling properties of SIS-PMMA bone cement were not significantly different from PMMA. The in vitro effect of PMMA and SIS-PMMA was investigated on MC3T3-E1 cells via the Transwell insert model to mimic the clinical condition or directly by culturing cells on the bone cement samples. The results indicated that SIS addition substantially enhanced the proliferation and osteogenic differentiation of MC3T3-E1 cells. Additionally, the bone cement's biomechanical properties were also assessed in a decalcified goat vertebrae model with a compression fracture, which indicated the SIS-PMMA had markedly increased compressive strength than PMMA. Furthermore, it was proved that the novel bone cement had good biosafety and efficacy based on the International Standards and guidelines. After 12 weeks of implantation, SIS-PMMA indicated significantly more osteointegration and new bone formation ability than PMMA. In addition, vertebral bodies with cement were also extracted for the uniaxial compression test, and it was revealed that compared with the PMMA-implanted vertebrae, the SIS-PMMA-implanted vertebrae had greatly enhanced maximum strength. Overall, these findings indicate the potential of SIS to induce efficient fixation between the modified cement surface and the host bone, thereby providing evidence that the SIS-PMMA bone cement is a promising filler for clinical vertebral augmentation.

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Polydopamine-modified decellularized intestinal scaffolds loaded with adipose-derived stem cells promote intestinal regeneration

Abstract: Regeneration of gastrointestinal tissues remains a great challenge due to their unique microenvironment. Functional composite decellularized scaffolds have shown great potential in gastrointestinal repair and inducing gastrointestinal tissue-specific proliferation. In...

Cited by 5 publications

References 65 publications

Biological scaffold as potential platforms for stem cells: Current development and applications in wound healing

Biological scaffold as potential platforms for stem cells: Current development and applications in wound healing

Adipose-Derived Mesenchymal Stromal Cells in Basic Research and Clinical Applications

Preclinical Evaluation of Bioactive Small Intestinal Submucosa-PMMA Bone Cement for Vertebral Augmentation

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