Polydopamine Nanoparticles Functionalized Electrochemical DNA Aptasensor for Serum Glycated Albumin Detection

Maraming, Pornsuda; Aye, Nang Noon Shean; Boonsiri, Patcharee; Daduang, Sakda; Buhome, Onanong; Daduang, Jureerut

doi:10.3390/ijms232213699

Cited by 5 publications

(5 citation statements)

References 49 publications

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“…Maraming's group detected GA using a polydopamine nanoparticle-functionalized electrochemical aptasensor. 28) The measurements of GA ranged from 15 to 15000 nM with a LOD of 6.0 nM. Furthermore, a sensing system of GA was designed using an electrode with reduced graphene oxide/Au nanoparticles/anti-GA aptamer.…”

Section: Resultsmentioning

confidence: 99%

Construction of Biosensing System for Glycated Albumin Using an Electron Transfer Peptide-Modified Protein Probe

Ito,

Sugawara

2024

CHEMICAL & PHARMACEUTICAL BULLETIN

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Glycated albumin (GA) is one of the proteins that replaces several sugar moieties and can be used as an indicator of diabetes mellitus. We developed a sensing system that uses GA in the early detection of diabetes mellitus. In this study, H 6 Y 4 C acetylated (Ac-) at the N-terminals of the peptide was combined with wheat germ agglutinin (WGA) to recognize glucose moieties. The Ac-H 6 Y 4 C-WGA was constructed as a GA-sensing probe. The tyrosine residues of Y 4 C exhibited an oxidation peak, and His-tag moieties were introduced to separate Ac-H 6 Y 4 C-WGA in the synthesis of the probe. The Ac-H 6 Y 4 C-WGA probe binds with the 1-2 molecules of Ac-H 6 Y 4 C per WGA using matrix assisted laser desorption/ionization-time of flight (MALDI-TOF)-MS. Next, the functions of Ac-H 6 Y 4 C-WGA were evaluated using voltammetry. The number of electrontransfers was calculated based on the relationship between the peak potential and logarithm of scan rate and was 3.03. In the electrochemical measurements with mannose and bovine serum albumin, the peak currents were similar to that of GA alone. By contrast, a decrease in the peak current was suppressed when glucose was added to the solution containing the probe. As a result, Ac-H 6 Y 4 C-WGA was selectively bound to the glucose moieties of GA. The calibration curve via differential pulse voltammetry was proportional to the concentrations of GA and ranged from 1.0 10 12 to 2.0 10 11 M with a detection limit of 3.3 10 13 M. Key words wheat germ agglutinin (WGA), his-tag, electron-transfer peptide, glycated albumin (GA)

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Section: Resultsmentioning

confidence: 99%

Construction of Biosensing System for Glycated Albumin Using an Electron Transfer Peptide-Modified Protein Probe

Ito,

Sugawara

2024

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…engineered a polydopamine‐based SNA electrochemical aptasensor for the detection of serum glycated albumin with the LOD of 0.4 µg mL −1 . [ 86 ] Furthermore, Chen et al. developed a polyA‐based aptamer nanobeacon for the highly efficient detection of ATP.…”

Section: Recent Biomedical Applications Of Stimuli‐responsive Sna Nan...mentioning

confidence: 99%

“…[18] In addition, Ye et al constructed a turn-on model based on a kanamycin-SNA aptasensor for monitoring antibiotic residues in foods with the LOD of 258 nm, [85] while Maraming et al engineered a polydopamine-based SNA electrochemical aptasensor for the detection of serum glycated albumin with the LOD of 0.4 μg mL −1 . [86] Furthermore, Chen et al developed a polyA-based aptamer nanobeacon for the highly efficient detection of ATP. Notably, they demonstrated that the dissociation constants and competitive binding kinetics of the engineered nanobeacon could be regulated by adjusting the polyA length to tune the sensitivity (Figure 1E).…”

Section: Snas Responsive To Other Stimulimentioning

confidence: 99%

Smart Stimuli‐Responsive Spherical Nucleic Acids: Cutting‐Edge Platforms for Biosensing, Bioimaging, and Therapeutics

Tang,

Zhao,

Luo

et al. 2024

Small

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Spherical nucleic acids (SNAs) with exceptional colloidal stability, multiple modularity, and programmability are excellent candidates to address common molecular delivery‐related issues. Based on this, the higher targeting accuracy and enhanced controllability of stimuli‐responsive SNAs render them precise nanoplatforms with inestimable prospects for diverse biomedical applications. Therefore, tailored diagnosis and treatment with stimuli‐responsive SNAs may be a robust strategy to break through the bottlenecks associated with traditional nanocarriers. Various stimuli‐responsive SNAs are engineered through the incorporation of multifunctional modifications to meet biomedical demands with the development of nucleic acid functionalization. This review provides a comprehensive overview of prominent research in this area and recent advancements in the utilization of stimuli‐responsive SNAs in biosensing, bioimaging, and therapeutics. For each aspect, SNA nanoplatforms that exhibit responsive behavior to both internal stimuli (including sequence, enzyme, redox reactions, and pH) and external stimuli (such as light and temperature) are highlighted. This review is expected to offer inspiration and guidance strategies for the rational design and development of stimuli‐responsive SNAs in the field of biomedicine.

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“…DA is one of the most important neurotransmitters in the human body and provides transmission of signals between nerve cells, and a deficiency of DA can cause many neurological and mental diseases [ 20 ]. It was reported that through the functionalization of polydopamine nanoparticles, an electrochemical aptasensor was developed for the rapid, accurate, and economical detection of glycated albumin as a promising biomarker for glycemic management [ 21 ]. There are also reports in the literature about the use of DA as a medication [ 7 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Polydopamine Coating of Graphitic Carbon Nitride, g-C3N4, Improves Biomedical Application

Sahiner,

Demirci,

Sahiner

2024

Biomedicines

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Graphitic carbon nitride (g-C3N4) is an intriguing nanomaterial that exhibits photoconductive fluorescence properties under UV–visible light. Dopamine (DA) coating of g-C3N4 prepared from melamine was accomplished via self-polymerization of DA as polydopamine (PDA). The g-C3N4 was coated with PDA 1, 3, and 5 times repeatedly as (PDA@g-C3N4) in tris buffer at pH 8.5. As the number of PDA coatings was increased on g-C3N4, the peak intensity at 1512 cm−1 for N–H bending increased. In addition, the increased weight loss values of PDA@g-C3N4 structures at 600 °C from TGA thermograms confirmed that the coating was accomplished. The band gap of g-C3N4, 2.72 eV, was reduced to 0.87 eV after five coatings with PDA. A pristine g-C3N4 was found to have an isoelectric point (IEP) of 4.0, whereas the isoelectric points of 1PDA@g-C3N4 and 3PDA@g-C3N4 are close to each other at 3.94 and 3.91, respectively. On the other hand, the IEP of 5PDA@g-C3N4 was determined at pH 5.75 assuming complete coating with g-C3N4. The biocompatibility of g-C3N4 and PDA@g-C3N4 against L929 fibroblast cell lines revealed that all PDA@g-C3N4 coatings were found to be biocompatible up to a 1000 mg/mL concentration, establishing that PDA coatings did not adversely affect the biocompatibility of the composite materials. In addition, PDA@g-C3N4 was screened for antioxidant potential via total phenol content (TPC) and total flavonoid content assays and it was found that PDA@g-C3N4 has recognizable TPC values and increased linearly with an increased number of PDA coatings. Furthermore, blood compatibility of pristine g-C3N4 is enhanced considerably upon PDA coating, affirmed by hemolysis and the blood clotting index%. Additionally, α-glucosidase inhibitory properties of PDA@g-C3N4 structures revealed that 67.6 + 9.8% of this enzyme was evenly inhibited by 3PDA@g-C3N4 structure.

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Polydopamine Nanoparticles Functionalized Electrochemical DNA Aptasensor for Serum Glycated Albumin Detection

Cited by 5 publications

References 49 publications

Construction of Biosensing System for Glycated Albumin Using an Electron Transfer Peptide-Modified Protein Probe

Construction of Biosensing System for Glycated Albumin Using an Electron Transfer Peptide-Modified Protein Probe

Smart Stimuli‐Responsive Spherical Nucleic Acids: Cutting‐Edge Platforms for Biosensing, Bioimaging, and Therapeutics

Polydopamine Coating of Graphitic Carbon Nitride, g-C3N4, Improves Biomedical Application

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