Polydopamine surface-modified hyperbranched polymeric nanoparticles for synergistic chemo/photothermal therapy of oral cancer

Abstract

A novel drug delivery system for the treatment of oral cancer was developed using a facile polydopamine (PDA)-based surface modification and a binding mechanism linking folic acid-targeting ligands. The system was able to achieve the following objectives: loading of chemotherapeutic agents, active targeting, pH responsiveness, and prolonged in vivo blood circulation. DOX-loaded polymeric nanoparticles (DOX/H20-PLA@PDA NPs) were functionalized with amino-poly (ethylene glycol)-folic acid (H2N-PEG-FA) after coating them with PDA to form the targeting combination, DOX/H20-PLA@PDA-PEG-FA NPs. The novel NPs exhibited drug delivery characteristics similar to DOX/H20-PLA@ PDA NPs. Meanwhile, the incorporated H2N-PEG-FA contributed to active targeting, as illustrated in cellular uptake assays and animal studies. In vitro cytotoxicity and in vivo anti-tumor studies have shown that the novel nanoplatforms exhibit extremely effective therapeutic effects. In conclusion, the multifunctional PDA-modified H20-PLA@PDA-PEG-FA NPs offer a promising chemotherapeutic strategy to improve the treatment of oral cancer.

Nano-Drug Delivery Systems in Oral Cancer Therapy: Recent Developments and Prospective

“… [ 111 ] H20-PLA@ PDA-PEG-FA NPs DOX (chemotherapy drug) and PDA (PTT agent) DOX-loaded polymeric NPs (DOX/H20-PLA@PDA NPs) were functionalized with amino-poly (ethylene glycol)-FA (H2N-PEG-FA) after coating them with PDA to form the targeting combination, DOX/H20-PLA@PDA-PEG-FA NPs. Photothermal effect and the pH sensitivity of the PDA films; chemotherapy effect of DOX Very effective therapeutic effect on OC, accelerated drug release in acidic TME under laser irradiation [ 62 ] PLGA-PEG nanocarriers ATRA- PLGA- PEG-programmed death-ligand 1 (PD-L1) nanomedicines ATRA-PLGA-PEG-PD-L1 nanomedicines were fabricated by loading ATRA into PLGA-PEG nanocarriers and modification using an anti-PD-L1 antibody ATRA-PLGA-PEG-PD-L1 NPs inhibited proliferation and induced apoptosis in cancer cells Rapid cellular uptake in DOK and CAL-27 cells, significantly inhibited proliferation and inducing apoptosis, specifically targeted tumor cells, enhanced anticancer activity and reduced side effects [ 66 ] FA-PEG-TK-PLGA NPs DOX PLGA, along with PEG, was used in the significant skeleton, and the ROS-responsive thioketone-containing (TK) was used for FA ligation to form FA-PEG-TK-PLGA NPs to load DOX DOX-induced apoptotic cell death Effective escape from endosomes, quick release of the entrapped DOX into the cytoplasm, induced apoptosis of OSCC cells [ 56 ] Poly (ethylene glycol)-poly (ε-caprolactone) copolymers (PEG-PCL) ICG (PDT PS) and an organic compound (C3) (PTT agent) C3 encapsulated in PEG-PCL with ICG to form hybrid NPs (PEGs-PCLs-C3s-ICG NPs) PEGs-PCLs-C3s-ICG NPs simultaneously produced hyperthermia through C3 and produced ROS with 808snm laser irradiation at tumor sites Better photothermal conversion stability, lower cytotoxicity, and a faster metabolic rate, which ensured the tumor elimination effect of PTT in vivo [ …”

“…FA receptor [56][57][58][59][60][61][62] Protein corona-modulating Tf2 peptide Transferrin receptor (TfR) [63] HN-1, a 12-amino acid peptide HN-1 receptor [64,65] Anti Programmed death-ligand 1 (PD-L1) antibody PD-L1 [66,67] α-tocopherol α-tocopherol receptor [68] Fucoidan Scavenger-A receptors (SR-A), L-selectin, Toll-like receptors, and PD-L1 [69] Antibodies specific for matrix metalloproteinase-1 (MMP-1) MMP-1 [70] pH-sensitive H-peptide Epidermal growth factor receptor (EGFR) [71] Anti-Her-2 (human epidermal growth factor receptor 2) nanobody Her-2 [72] Dental pulp mesenchymal stem cell (DPSC), which expresses the CXCL8 binding receptor, CXCR2 Chemokine CXCL8 [73] Chemokine SDF-1 CXC chemokine receptor 4 (CXCR4) [74] Shiga Toxin-B Globotriaosylceramide receptor (GB3) [75] Fibroblast activation protein (FAP)-targeted peptide chains FAP [76] AE105 (H-Asp-Cha-Phe-(d)Ser-(d)Arg-Tyr-Leu-Trp-SerOH) peptide Urokinase plasminogen activator receptor (uPAR) [77]…”

“…Photothermal effect and the pH sensitivity of the PDA films; chemotherapy effect of DOX Very effective therapeutic effect on OC, accelerated drug release in acidic TME under laser irradiation [62]…”