Polyelectrolyte coating of ferumoxytol nanoparticles for labeling of dendritic cells

Celikkin, Nehar; Jakubcová, Lucie; Zenke, Martin; Hoß, Mareike; Wong, John E.; Hieronymus, Thomas

doi:10.1016/j.jmmm.2014.09.001

Cited by 4 publications

(22 citation statements)

References 29 publications

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“…In contrast, GM-DCs showed significantly higher labeling efficiency by uncoated ferumoxytol than FL-DCs. The PE-coating of MNPs resulted in increased cell labeling in line with previous studies but with different impact [16,27]. For FL-DCs, the MNP coating with both PEI and PDADMAC resulted in a four-fold higher labeling of FL-DCs than with uncoated MNPs (Figure 3A).…”

Section: Resultssupporting

confidence: 87%

“…We determined for ferumoxytol a hydrodynamic diameter of 29 nm and a ζ-potential of - 44 mV (Figure 1A and B). We frequently use layer-by-layer (LbL) assembly of high and low molecular weight (MW) polyelectrolytes around MNPs to improve cellular responses, including uptake, intracellular localization and processing of MNPs, and thus on MRI properties of labeled cells [16,18].…”

Section: Resultsmentioning

confidence: 99%

“…High MW polyethyleneimine (PEI, 750 kDa) and low MW polydiallyldimethylammoniumchlorid (PDADMAC, 100-200 kDa) were obtained from Sigma-Aldrich (Taufkirchen, Germany). The surface coating of ferumoxytol was carried out using the LbL technique to deposit polyelectrolyte layers (Figure 1 A) [16,[18][19][20]. The negatively charged ferumoxytol MNPs were added to an aqueous solution of the positively charged polyelectrolytes to prepare the coating layer.…”

Section: Polyelectrolyte Coating Of Ferumoxytolmentioning

confidence: 99%

“…Ferumoxytol has also been used as a macrophage-imaging agent as well as a blood-pool agent with MRI [14]. However, ferumoxytol alone did not result in effective cell labeling [15,16]. Thus, in previous studies, we successfully established conditions that enabled us to manufacture colloidal stable ferumoxytol particles coated with polyelectrolytes (PE), resulting in enhanced cell labeling [16].…”

Section: Introductionmentioning

confidence: 99%

“…However, ferumoxytol alone did not result in effective cell labeling [15,16]. Thus, in previous studies, we successfully established conditions that enabled us to manufacture colloidal stable ferumoxytol particles coated with polyelectrolytes (PE), resulting in enhanced cell labeling [16]. In the present study, we mainly focused on the impact of PE-coated ferumoxytol MNPs on the labeling of different DC subsets, including inflammatory DCs, and steady-state conventional DCs (cDCs) and plasmacytoid DCs (pDCs) [17].…”

Section: Introductionmentioning

confidence: 99%

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Polyelectrolyte Coating of Ferumoxytol Differentially Impacts on Labeling of Inflammatory and Steady-State Dendritic Cell Subtypes

Celikkin

Zenke

et al. 2021

Preprint

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Engineered magnetic nanoparticles (MNPs) are emerging as advanced tools for medical applications. The coating of MNPs using polyelectrolytes (PEs) is a versatile means to tailor MNP properties and is used to optimize MNP functionality. Dendritic cells (DCs) are critical regulators of adaptive immune responses. Functional distinct DC subsets exist either under steady-state or inflammatory conditions, which are explored for the specific treatment of various diseases, such as cancer, autoimmunity or transplant rejection. Here, the impact of PE coating of ferumoxytol for uptake into both inflammatory and steady-state DCs and cellular responses to the MNP labeling is addressed. Labeling efficiency by uncoated and PE-coated ferumoxytol is highly variable in different DC subsets, and PE coating significantly improves the labeling of steady-state DCs. Uncoated ferumoxytol results in increased cytotoxicity of steady-state DCs after labeling that is abolished by the PE coating, while no increased cell death is observed in inflammatory DCs. Furthermore, uncoated and PE-coated ferumoxytol appears immunologically inert in inflammatory DCs but induces activation of steady-state DCs. These results show that PE coating of MNPs can be applied to endow particles with desired properties for enhanced uptake and cell type-specific responses in distinct target DC populations.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Polyelectrolyte Coating Of Ferumoxytolmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Polyelectrolyte Coating of Ferumoxytol Differentially Impacts on Labeling of Inflammatory and Steady-State Dendritic Cell Subtypes

Celikkin

Zenke

et al. 2021

Preprint

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Effect of reaction temperature on electrical and magnetic properties of chemically synthesized MnS nanocrystals

Veeramanikandasamy¹,

Rajendran²,

Sambath³

2019

Solid State Sciences

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Polyelectrolyte Coating of Ferumoxytol Differentially Impacts the Labeling of Inflammatory and Steady-State Dendritic Cell Subtypes

et al. 2022

Self Cite

View full text Add to dashboard Cite

Engineered magnetic nanoparticles (MNPs) are emerging as advanced tools for medical applications. The coating of MNPs using polyelectrolytes (PEs) is a versatile means to tailor MNP properties and is used to optimize MNP functionality. Dendritic cells (DCs) are critical regulators of adaptive immune responses. Functionally distinct DC subsets exist, either under steady-state or inflammatory conditions, which are explored for the specific treatment of various diseases, such as cancer, autoimmunity, and transplant rejection. Here, the impact of the PE coating of ferumoxytol for uptake into both inflammatory and steady-state DCs and the cellular responses to MNP labeling is addressed. Labeling efficiency by uncoated and PE-coated ferumoxytol is highly variable in different DC subsets, and PE coating significantly improves the labeling of steady-state DCs. Uncoated ferumoxytol results in increased cytotoxicity of steady-state DCs after labeling, which is abolished by the PE coating, while no increased cell death is observed in inflammatory DCs. Furthermore, uncoated and PE-coated ferumoxytol appear immunologically inert in inflammatory DCs, but they induce activation of steady-state DCs. These results show that the PE coating of MNPs can be applied to endow particles with desired properties for enhanced uptake and cell type-specific responses in distinct target DC populations.

show abstract

Polyelectrolyte coating of ferumoxytol nanoparticles for labeling of dendritic cells

Cited by 4 publications

References 29 publications

Polyelectrolyte Coating of Ferumoxytol Differentially Impacts on Labeling of Inflammatory and Steady-State Dendritic Cell Subtypes

Polyelectrolyte Coating of Ferumoxytol Differentially Impacts on Labeling of Inflammatory and Steady-State Dendritic Cell Subtypes

Effect of reaction temperature on electrical and magnetic properties of chemically synthesized MnS nanocrystals

Polyelectrolyte Coating of Ferumoxytol Differentially Impacts the Labeling of Inflammatory and Steady-State Dendritic Cell Subtypes

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