Polyelectrolyte Complex Consisting Sodium Dextran Sulfate and 2-(Diethylamino)ethyldextran Hydrochloride

康男, 菊池; 洋, 福田

doi:10.1246/nikkashi.1977.1051

Cited by 2 publications

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“…A procedure by Yoshioka et al (1990) produces microcapsules by the interaction of the ionic polysaccharides (carboxymethy1)cellulose and chitosan in a process termed complex coacervation. The complex coacervation concept is attractive because of its simplicity, gentle nature, and the availability of a large number of biocompatible ionic polymers and polysaccharides capable of interacting to form membranes (Fukudaand Kikuchi, 1979;Kikuchi and Fukuda, 1977;Takahashi et al, 1990;Kikuchi and Noda, 1976;Chavasit and Torres, 1990). A number of the polysaccharides are known to be essential components of connective tissue and cellular basement membranes (Lindahl and Hook, 1978), and they raise the possibility of developing a microcapsule whose components would actively promote the attachment and function of the encapsulated cells.…”

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confidence: 99%

Complex Coacervate Microcapsules for Mammalian Cell Culture and Artificial Organ Development

Matthew

Salley

Peterson

et al. 1993

Biotechnology Progress

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A number of combinations of anionic and cationic polymers, the majority being polysaccharides, were screened to determine their suitability for the development of alternative microcapsule formulations capable of supporting cells. The capsules were taken through a limited optimization and then evaluated on the bases of rupture strength, permeability to albumin, and ability of their components to promote the attachment, aggregation, and function of encapsulated rabbit hepatocytes. The widely used alginate-polylysine capsules were employed as a comparative standard in all tests. A number of the new formulations compared favorably with the standard, and some exhibited superior performance in specific areas. Hepatocyte function, as evaluated by the rate of urea synthesis, showed no significant differences between formulations over a 24-h test period. One formulation, composed of the polysaccharides (carboxymethyl)cellulose, chondroitin sulfate A, chitosan, and polygalacturonate, was found to be superior to alginate-polylysine capsules in the areas investigated and supported the long-term survival and growth of liver endothelial cells.

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