Polyelectrolyte multilayer coatings for the separation of proteins by capillary electrophoresis: Influence of polyelectrolyte nature and multilayer crosslinking

Bekri, Samya; Leclercq, Laurent; Cottet, Hervé

doi:10.1016/j.chroma.2015.04.033

Cited by 45 publications

(41 citation statements)

References 36 publications

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“…The application of a multilayer ionic polymer for the improvement of protein separation performances in CZE mode was investigated. 38 A systematic study comparing a broad library based on nine different polyanions and eight different polycations was undertaken. Among the 72 possible polycation/polyanion couples, one third (23 couples) were evaluated for the separation of a test mixture of five intact proteins.…”

Section: Separation Modes and Conditionsmentioning

confidence: 99%

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Recent advances in protein analysis by capillary and microchip electrophoresis

Dawod

Arvin

Kennedy

2017

Analyst

120

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This review article describes the significant recent advances in analysis of proteins by capillary and microchip electrophoresis during the period mid 2014 to early 2017. The review highlights the progressions, new methodologies, innovative instrumental modifications, and challenges for efficient protein analysis in human specimens, animal tissues, and plant samples. Protein analysis fields covered in this review include analysis of native, reduced, and denatured proteins in addition to Western blotting, protein therapeutics and proteomics.

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Section: Separation Modes and Conditionsmentioning

confidence: 99%

“…Hydrodynamic injection: 30 mbar, 4 s. Temperature: 25°C. (Reproduced from reference 38 with permission)…”

Section: Figurementioning

confidence: 99%

Recent advances in protein analysis by capillary and microchip electrophoresis

Dawod

Arvin

Kennedy

2017

Analyst

120

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“…In the CE of proteins, choosing the proper capillary wall coating is an essential part of method development [1][2][3][4][5][6][7][8]. The coatings reduce the notorious protein adsorption at the walls that lead to severe analyte band broadening and fouling of the fused-silica capillary, as noted by recent reviews on the use of CE for protein separations [1,3,9,10].…”

Section: Introductionmentioning

confidence: 99%

A cationic β‐cyclodextrin as a dynamic coating for the separation of proteins in capillary electrophoresis

Quirino

2017

J of Separation Science

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A cationic cyclodextrin was used as dynamic coating for the capillary electrophoresis of a model mixture of proteins (i.e., ubiquitin, α-lactoglobulin, cytochrome-c, and myoglobin) as positively charged species in a fused silica capillary. An interesting feature of the coating is that by simple adjustment of the concentration of cyclodextrin added into the background electrolyte, a neutral or positively charged surface, which was beneficial in preventing protein adsorption at the inner capillary wall surface, was obtained. This is the first demonstration of a dynamic coating that yielded a neutral surface for protein separations in capillary electrophoresis. Based on electro-osmotic flow measurements, addition of 0.05 to 0.10 mg/mL quaternary β-cyclodextrin in a low pH electrolyte resulted in a neutral or positive surface (undetectable to very slow anodic electro-osmotic flow). The coating approach afforded the electrophoretic separation of the mixture of proteins at positive polarity with good repeatability and separation performance.

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“…proposed noncovalent cation coating , which comprises successive attaching of cationic and anionic polymers to the capillary wall. When cationic polymer forms the final layer, the EOF is reversed and adsorption of cationic molecules is prevented .…”

Section: Introductionmentioning

confidence: 99%

Estimation of acidity constants, ionic mobilities and charges of antimicrobial peptides by capillary electrophoresis

et al. 2016

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Capillary electrophoresis (CE) was employed for the determination of thermodynamic acidity constants (pK ) and actual ionic mobilities of polycationic antimicrobial peptides (AMPs). The effective electrophoretic mobilities of AMPs were measured by CE in a series of the background electrolytes within a wide pH range (2.00-12.25), at constant ionic strength (25 mM) and ambient temperature, using polybrene coated fused silica capillaries to suppress sorption of cationic AMPs to the capillary wall. Eventually, Haarhoff-Van der Linde peak fitting function was used for the determination of correct migration times of some AMPs peaks that were distorted by electromigration dispersion. The measured effective mobilities were corrected to 25°C. Mixed acidity constants, pKa,i mix , and actual ionic mobilities, m , of AMPs were determined by the nonlinear regression analysis of pH dependence of their effective mobilities. The pKa,i mix values were recalculated to thermodynamic pK s using the Debye-Hückel theory. Thermodynamic pK of imidazolium group of histidine residues was found to be in the range 3.72-4.98, pK of α-NH group was in the range 6.14-6.93, and pK of ε-NH group of lysine spanned the interval 7.26-9.84, depending on the particular amino acid sequence of the AMPs. Actual ionic mobilities of AMPs with positive charges from one to six elementary units achieved values (9.8 - 36.5) × 10 m V s .

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Polyelectrolyte multilayer coatings for the separation of proteins by capillary electrophoresis: Influence of polyelectrolyte nature and multilayer crosslinking

Cited by 45 publications

References 36 publications

Recent advances in protein analysis by capillary and microchip electrophoresis

Recent advances in protein analysis by capillary and microchip electrophoresis

A cationic β‐cyclodextrin as a dynamic coating for the separation of proteins in capillary electrophoresis

Estimation of acidity constants, ionic mobilities and charges of antimicrobial peptides by capillary electrophoresis

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