Polyelectrolyte multilayers of poly (<scp>l</scp>-lysine) and hyaluronic acid on nanostructured surfaces affect stem cell response

Niepel, Marcus S.; Ekambaram, Bhavya K; Schmelzer, Christian E.H.; Groth, Thomas

doi:10.1039/c8nr05529g

Cited by 22 publications

(21 citation statements)

References 61 publications

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“…Parallel ridge stripes that mimic the shape of vascular endothelial cells (EC) when exposed to the shear stress from the blood flow, generated under physiological conditions, were used to study the adhesion of EC and smooth muscle cells (SMCs) on high M w HA-modified stripes [50]. More recently, -(PEI-[HA-PLL] 11 -HA) hexagonally arranged nanostructures with crosslinked polyelectrolyte multilayer with defined spacing (i.e., 733, 518, and 302 nm) and tuned mechanical properties were used to differentiate human adipose-derived stem cells, showing that highly crosslinked surfaces presenting HA-coated particles of smaller size (i.e., 302 nm) induced osteogenesis [51].…”

Section: D Surfacesmentioning

confidence: 99%

Extracellular Matrix Mimics Using Hyaluronan-Based Biomaterials

Amorim

Reis

et al. 2021

Trends in Biotechnology

125

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Hyaluronan (HA) is a critical element of the extracellular matrix (ECM). The regulated synthesis and degradation of HA modulates the ECM chemical and physical properties that, in turn, influence cellular behavior. HA triggers signaling pathways associated with the adhesion, proliferation, migration, and differentiation of cells, mediated by its interaction with specific cellular receptors or by tuning the mechanical properties of the ECM. This review summarizes the recent advances on strategies used to mimic the HA present in the ECM to study healthy or pathological cellular behavior. This includes the development of HA-based 2D and 3D in vitro tissue models for the seeding and encapsulation of cells, respectively, and HA particles as carriers for the targeted delivery of therapeutic agents. Highlights The extracellular matrix is composed of hyaluronan of different molecular weights that play different roles in cellular proliferation, migration, and differentiation.

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Section: D Surfacesmentioning

confidence: 99%

Extracellular Matrix Mimics Using Hyaluronan-Based Biomaterials

Amorim

Reis

et al. 2021

Trends in Biotechnology

125

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“…193 A strong interdependence of all properties might be the reason, why studies systematically investigating the effect of altered topography and mechanical characteristics are rare. [216][217][218][219][220][221][222]…”

Section: Materials Functionalization With Various Biochemical Groupsmentioning

confidence: 99%

Multifunctional Biomaterials: Combining Material Modification Strategies for Engineering of Cell-Contacting Surfaces

Mertgen

Trossmann

Guex

et al. 2020

ACS Appl. Mater. Interfaces

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In the human body, cells in a tissue are exposed to signals derived from their specific extracellular matrix (ECM) cues from architectural structure, mechanical properties and chemical composition (proteins, growth factors). Research on biomaterials, in tissue engineering and regenerative medicine aim to recreate such stimuli with engineered materials in order to induce a specific response of cells at the interface. While traditional biomaterials design has been mostly limited to varying individual cues, increasing interest has arisen on combining several features in recent years in order improve the mimicry of extracellular matrix properties. Tremendous progress in

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“…Further studies were carried out with PVP 5%-HA 0.8% w/v nanofibers which were crosslinked with ε-PL taking benefit of the electrostatic interactions among the two polymers. Indeed, ε-PL is able to interact by electrostatic interactions with anionic polymers, and HA and ε-PL interactions have been already exploited for obtaining polyelectrolyte multilayers [46][47][48]. Apart from layer-by-layer structures, ε-PL is expected to interact with hyaluronan thanks to inter-and intra-molecular linkages between ε-PL amino groups and hyaluronan carboxylic groups forming macro-, micro-or nanohydrogels.…”

Section: Ocular Inserts Of Nanofibers Crosslinked With ε-Polylysinementioning

confidence: 99%

Crosslinked Hyaluronan Electrospun Nanofibers for Ferulic Acid Ocular Delivery

Grimaudo

Concheiro

2020

Pharmaceutics

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Electrospun nanofibers are gaining interest as ocular drug delivery platforms that may adapt to the eye surface and provide sustained release. The aim of this work was to design an innovative ophthalmic insert composed of hyaluronan (HA) nanofibers for the dual delivery of an antioxidant (ferulic acid, FA) and an antimicrobial peptide (ε-polylysine, ε-PL). Polyvinylpyrrolidone (PVP) was added to facilitate the electrospinning process. Fibers with diameters of approx. 100 nm were obtained with PVP 5%-HA 0.8% w/v and PVP 10%-HA 0.5% w/v mixtures in ethanol:water 4:6 v/v. An increase in PVP concentration to 20% w/v in both presence and absence of HA rendered fibers of approx. 1 µm. PVP 5%-HA 0.8% w/v fibers were loaded with 83.3 ± 14.0 µg FA per mg. After nanofibers crosslinking with ε-PL, blank and FA-loaded inserts showed a mean thickness of 270 ± 21 µm and 273 ± 41 µm, respectively. Blank and FA-loaded inserts completely released ε-PL within 30 min under sink conditions, whereas FA-loaded inserts released the antioxidant within 20 min. Both blank and FA-loaded inserts were challenged against Pseudomonas aeruginosa and Staphylococcus aureus, demonstrating their efficacy against relevant microbial species.

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Polyelectrolyte multilayers of poly (l-lysine) and hyaluronic acid on nanostructured surfaces affect stem cell response

Abstract: Laser interference lithography (LIL) and layer-by-layer (LbL) technique are combined for the first time to design exceptional systems for control of stem cell fate.

Cited by 22 publications

References 61 publications

Extracellular Matrix Mimics Using Hyaluronan-Based Biomaterials

Extracellular Matrix Mimics Using Hyaluronan-Based Biomaterials

Multifunctional Biomaterials: Combining Material Modification Strategies for Engineering of Cell-Contacting Surfaces

Crosslinked Hyaluronan Electrospun Nanofibers for Ferulic Acid Ocular Delivery

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