Polyester copolymer scaffolds enhance expression of bone markers in osteoblast‐like cells

Idris, Shaza Bushra; Arvidson, Kristina; Plikk, Peter; Ibrahim, Mohamed Yousif; Finne‐Wistrand, Anna; Albertsson, Ann‐Christine; Bolstad, Anne Isine; Mustafa, Kamal

doi:10.1002/jbm.a.32726

Cited by 38 publications

(42 citation statements)

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“…The pattern of ECM production exhibited by the HOBs is similar to that observed by other groups using different types of scaffolds, including poly( l -lactide) (PLLA) copolymers, RADA-16, PEG and gelatin gels. 10,22,30,31 …”

Section: Resultsmentioning

confidence: 99%

Human osteoblasts within soft peptide hydrogels promote mineralisation in vitro

et al. 2014

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Biomaterials that provide three-dimensional support networks for the culture of cells are being developed for a wide range of tissue engineering applications including the regeneration of bone. This study explores the potential of the versatile ionic-complementary peptide, FEFEFKFK, for such a purpose as this peptide spontaneously self-assembles into β-sheet-rich fibres that subsequently self-associate to form self-supporting hydrogels. Via simple live/dead cell assays, we demonstrated that 3 wt% hydrogels were optimal for the support of osteoblast cells. We went on to show that these cells are not only viable within the three-dimensional hydrogel but they also proliferate and produce osteogenic key proteins, that is, they behave like in vivo bone cells, over the 14-day period explored here. The gel elasticity increased over time when cells were present – in comparison to a decrease in control samples – indicating the deposition of matrix throughout the peptide scaffold. Moreover, significant quantities of calcium phosphate were deposited. Collectively, these data demonstrate that ionic-complementary octapeptides offer a suitable three-dimensional environment for osteoblastic cell function.

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Section: Resultsmentioning

confidence: 99%

Human osteoblasts within soft peptide hydrogels promote mineralisation in vitro

et al. 2014

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“…Several authors have shown that various scaffolding materials, including polymer, can induce ectopic bone formation [8,11,12]. In vitro findings have also suggested that poly(LLA-co-DXO) scaffolds enhance differentiation of osteogenic cells [13]. …”

Section: Introductionmentioning

confidence: 99%

Endothelial microvascular networks affect gene-expression profiles and osteogenic potential of tissue-engineered constructs

et al. 2013

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IntroductionA major determinant of the potential size of cell/scaffold constructs in tissue engineering is vascularization. The aims of this study were twofold: first to determine the in vitro angiogenic and osteogenic gene-expression profiles of endothelial cells (ECs) and mesenchymal stem cells (MSCs) cocultured in a dynamic 3D environment; and second, to assess differentiation and the potential for osteogenesis after in vivo implantation.MethodsMSCs and ECs were grown in dynamic culture in poly(L-lactide-co-1,5-dioxepan-2-one) (poly(LLA-co-DXO)) copolymer scaffolds for 1 week, to generate three-dimensional endothelial microvascular networks. The constructs were then implanted in vivo, in a murine model for ectopic bone formation. Expression of selected genes for angiogenesis and osteogenesis was studied after a 1-week culture in vitro. Human cell proliferation was assessed as expression of ki67, whereas α-smooth muscle actin was used to determine the perivascular differentiation of MSCs. Osteogenesis was evaluated in vivo through detection of selected markers, by using real-time RT-PCR, alkaline phosphatase (ALP), Alizarin Red, hematoxylin/eosin (HE), and Masson trichrome staining.ResultsThe results show that endothelial microvascular networks could be generated in a poly(LLA-co-DXO) scaffold in vitro and sustained after in vivo implantation. The addition of ECs to MSCs influenced both angiogenic and osteogenic gene-expression profiles. Furthermore, human ki67 was upregulated before and after implantation. MSCs could support functional blood vessels as perivascular cells independent of implanted ECs. In addition, the expression of ALP was upregulated in the presence of endothelial microvascular networks.ConclusionsThis study demonstrates that copolymer poly(LLA-co-DXO) scaffolds can be prevascularized with ECs and MSCs. Although a local osteoinductive environment is required to achieve ectopic bone formation, seeding of MSCs with or without ECs increases the osteogenic potential of tissue-engineered constructs.

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“…Biocompatibility was confirmed according to ISO/EN 109935 guidelines [6] and a series of studies has demonstrated that cell lines with osteoprogenitor potential, such as rat-and human-derived bone marrow stromal cells (MSC), periodontal ligament cells and human osteoblast-like cells, attach, spread and proliferate well on these constructs [7][8][9][10]. Moreover, the scaffolds stimulate osteogenic differentiation of rat and human bone marrow stromal cells and human osteoblastlike cells [7,8,10]. A recent animal study, using a rat calvarial defect model, showed that implantation of scaffolds seeded with endothelial cells and MSC promoted rapid bone formation [11].…”

Section: Introductionmentioning

confidence: 99%