Polyester hydrolytic and synthetic activity catalyzed by the medium-chain-length poly(3-hydroxyalkanoate) depolymerase from Streptomyces venezuelae SO1

Santos, Marta Maria Oliveira dos; Gangoiti, Joana; Keul, Helmut; Möller, Martin; Serra, Juan L.; Llama, Marı́a J.

doi:10.1007/s00253-012-4210-1

Cited by 28 publications

(24 citation statements)

References 34 publications

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“…However, after 3 days of reaction at 30°C, the enzyme was unable to hydrolyze olive oil, which is a suitable substrate for lipases, indicating that this depolymerase does not show lipase activity (data not shown). Similar substrate specificity was observed with PhaZ SveSO1 (47) and PhaZ SomSO2 (unpublished data). As described before (14), the prototype PhaZ PflGK13 did not hydrolyze scl PHA or PLA.…”

Section: Resultssupporting

confidence: 60%

“…On the other hand, its activity with scl pNP-alkanoates was significantly lower. Similar substrate specificities for pNP-alkanoates were described for PhaZ SspKJ-72 (23) and PhaZ SveSO1 (47). In contrast, PhaZ PflGK13 showed maximum esterase activity when pNPtetradecanoate was used as the substrate (8).…”

Section: Resultsmentioning

confidence: 73%

“…Polymer-degrading bacteria were routinely grown in solid M9 mineral medium (45) containing 1.5% (wt/vol) agar with the carbon sources indicated in the text. For enzyme production, S. roseolus SL3 and S. venezuelae SO1 cells were grown at 30°C in 250-ml Erlenmeyer flasks containing 100 ml of mineral medium supplemented with a film (0.15 g) of P(3HO) as the sole carbon and energy source, as described by Santos et al (47). The strains were maintained as frozen spore suspensions in 15% (vol/vol) glycerol at Ϫ20°C, as described by Kieser et al (19).…”

Section: Chemicalsmentioning

confidence: 99%

“…In a recent study, the identification of a significantly different mcl PHA depolymerase gene from the thermophilic bacterium Thermus thermophilus HB8 has been reported (36). Recently, the isolation and identification of Streptomyces venezuelae SO1 as a novel mcl PHA depolymerase (PhaZ SveSO1 ) producer have been reported by our group (47). However, the molecular characteristics of the genes encoding mcl PHA depolymerases from Streptomyces origins have not yet been cleared.…”

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confidence: 99%

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Characterization of a Novel Subgroup of Extracellular Medium-Chain-Length Polyhydroxyalkanoate Depolymerases from Actinobacteria

Gangoiti

Santos

Prieto³

et al. 2012

Appl Environ Microbiol

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c Nineteen medium-chain-length (mcl) poly(3-hydroxyalkanoate) (PHA)-degrading microorganisms were isolated from natural sources. From them, seven Gram-positive and three Gram-negative bacteria were identified. The ability of these microorganisms to hydrolyze other biodegradable plastics, such as short-chain-length (scl) PHA, poly(-caprolactone) (PCL), poly(ethylene succinate) (PES), and poly(L-lactide) (PLA), has been studied. On the basis of the great ability to degrade different polyesters, Streptomyces roseolus SL3 was selected, and its extracellular depolymerase was biochemically characterized. The enzyme consisted of one polypeptide chain of 28 kDa with a pI value of 5.2. Its maximum activity was observed at pH 9.5 with chromogenic substrates. The purified enzyme hydrolyzed mcl PHA and PCL but not scl PHA, PES, and PLA. Moreover, the mcl PHA depolymerase can hydrolyze various substrates for esterases, such as tributyrin and p-nitrophenyl (pNP)-alkanoates, with its maximum activity being measured with pNP-octanoate. Interestingly, when poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate [11%]) was used as the substrate, the main hydrolysis product was the monomer (R)-3-hydroxyoctanoate. In addition, the genes of several Actinobacteria strains, including S. roseolus SL3, were identified on the basis of the peptide de novo sequencing of the Streptomyces venezuelae SO1 mcl PHA depolymerase by tandem mass spectrometry. These enzymes did not show significant similarity to mcl PHA depolymerases characterized previously. Our results suggest that these distinct enzymes might represent a new subgroup of mcl PHA depolymerases.

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Section: Resultssupporting

confidence: 60%

Section: Resultsmentioning

confidence: 73%

Section: Chemicalsmentioning

confidence: 99%

mentioning

confidence: 99%

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Characterization of a Novel Subgroup of Extracellular Medium-Chain-Length Polyhydroxyalkanoate Depolymerases from Actinobacteria

Gangoiti

Santos

Prieto³

et al. 2012

Appl Environ Microbiol

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“…PHB depolymerases can be employed for the production of esters in anhydrous media [110][111][112][113]. Similarly, PHA depolymerases from Pseudomonas fluorescens GK13 and Streptomyces venezuelae SO1 were applied to esterification and PHB synthesis [114,115].…”

Section: Applications Of Phb Depolymerasesmentioning

confidence: 99%

Biodegradable Polymers: Recent Developments and New Perspectives

Carrasco¹,

Pérez²,

Cailloux³

et al. 2017

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PHB (poly‐β‐hydroxybutyrate) and its enzymatic degradation

Roohi

Zaheer²,

Kuddus

2017

Polymers for Advanced Techs

116

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Our daily life needs depend on plastics, as they are cheap and durable, so they become the most commonly used synthetic chemical products. But from an environmentalist's point of view, a major concern related to these plastics is their non-biodegradable nature. Driven by growing demand to search for sustainable solutions to dispose off generating huge volume of synthetic plastic wastes, shifted the mind of researcher towards the use of biodegradable plastics which can be completely disposed-off by microbial enzymatic degradation. These biodegradable plastics or "bioplastics" are also synthesized by microbes under certain stressed environmental conditions out of which poly(R-3-hydroxybutyrate) (PHB) is the most ubiquitous and best known representatives of polyhydroxyalkanoate family. The PHB is most intensively used for the innovative biomedical applications owing to suitable combination of biocompatibility, transport characteristics, and mechanical properties. These challenging aspects of PHB can be used for designing of novel medical devices, in tissue engineering, and for systematic sustained drug delivery. Lots of research reports on PHB degrading enzymes and their producing microorganisms including biochemical aspects are available but in scattered form. So this review highlighted all the relevant information of PHB and PHB-degrading enzymes starting with basic classification, synthesis, mechanism, and applications that are environment friendly and are of public interest.

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Polyester hydrolytic and synthetic activity catalyzed by the medium-chain-length poly(3-hydroxyalkanoate) depolymerase from Streptomyces venezuelae SO1

Cited by 28 publications

References 34 publications

Characterization of a Novel Subgroup of Extracellular Medium-Chain-Length Polyhydroxyalkanoate Depolymerases from Actinobacteria

Characterization of a Novel Subgroup of Extracellular Medium-Chain-Length Polyhydroxyalkanoate Depolymerases from Actinobacteria

Biodegradable Polymers: Recent Developments and New Perspectives

PHB (poly‐β‐hydroxybutyrate) and its enzymatic degradation

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