Polyester Sheeting (Melinex O), a Tissue-Culture Support Easily Separable from Epoxy Resins after Flat-Face Embedding

Firket, H

doi:10.3109/10520296609116304

Cited by 41 publications

(10 citation statements)

References 7 publications

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“…Sections were examined with a Siemen's Elmiskop IA electron microscope (Siemens America, Inc, New York). Macrophages adhering to the Melinex plastic film were processed by the method of Firket (12).…”

Section: Methodsmentioning

confidence: 99%

The human alveolar macrophage: isolation, cultivation in vitro, and studies of morphologic and functional characteristics

Cohen¹,

Cline²

1971

J. Clin. Invest.

177

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A B S T R A C T Human alveolar macrophages were lavaged from surgically resected lungs and from lungs of normal subjects. Macrophages that had been purified by glass adherence were maintained in tissue culture for as long as 54 days. After 3-4 wk in vitro they underwent transformation into multinucleated giant cells. These aged cells had more than 30 times the phagocytic capacity that the same group of cells had had after 1 day in vitro.Phagocytosis of heat-killed Candida albicans was inhibited by iodoacetate, sodium fluoride, potassium cyanide, and low partial pressures of oxygen, suggesting that these cells require both oxidative and glycolytic energy sources for maximal particle ingestion. Alveolar macrophages and monocyte-derived macrophages killed Listeria monocytogenes with similar efficiency, but neutrophils were more efficient than either of the other cell types. Bacterial killing is probably not dependent upon myeloperoxidase in the monocyte-derived macrophage or in the alveolar macrophage since histochemical stains for peroxidase do not stain either cell type. C. albicans blastospores, which are killed by neutrophils and monocytes that contain myeloperoxidase, were not killed by human alveolar macrophages during the 4 hr of observation.Large cells with supernormal phagocytic capacity were recovered from patients with postobstructive pheumonia and from one patient with recurrent bacterial pneumonia, indicating that macrophage function can be altered in certain disease states.Human alveolar macrophages are unique human phagocytes in their dependence on an oxygen tension greater than 25 mm HG for maximal phagocytosis. Carbon dioxide tensions as high as 70 mm Hg did not alter phago-

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Section: Methodsmentioning

confidence: 99%

The human alveolar macrophage: isolation, cultivation in vitro, and studies of morphologic and functional characteristics

Cohen¹,

Cline²

1971

J. Clin. Invest.

177

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show abstract

“…Seven cultures were incubated in 'Leighton' tubes containing a sheet of Melinex (I.C.I. ), a transparent polyester (Firket, 1966). In each case the cells attached and spread out on the Melinex sheet.…”

Section: Cell Culturesmentioning

confidence: 99%

Ultrastructure of synovial cells in vitro.

Glen-Bott¹

1972

Annals of the Rheumatic Diseases

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“…An alternative approach has been to grow the cell cultures on other supports which can be more readily detached from the embedding resins. Kjellen et al Clegg (1964) and Firket (1966) reported the use of polyester sheeting (Melinex), but according to Clegg the surface was hydrophobic and cells attached to it with difficulty. It was therefore necessary to coat the material with carbon.…”

Section: Introductionmentioning

confidence: 99%