Polyethylene glycol effects on somatic embryogenesis of papaya hybrid UENF/CALIMAN 01 seeds

Heringer, Angelo Schuabb; Vale, Ellen Moura; Barroso, Tatiana; Santa–Catarina, Claudete; Silveira, Vanildo

doi:10.1590/s2197-00252013000200004

Cited by 26 publications

(28 citation statements)

References 38 publications

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“…Studies have described the ability of PEG to increase the osmolarity of the medium and increase the frequency of somatic embryo induction (Castillón and Kamo 2002, Vooková and Kormuták 2002, Steiner et al 2005, Alkhateeb 2006, Calic-Dragosavac et al 2010, Vale et al 2014. The results achieved with PEG may be related to the non-plasmolyzing osmotic effect of this compound: PEG may be able to induce water stress even if no water actually enters the plant cell (Alkhateeb 2006), and lead to an increased protein synthesis (Heringer et al 2013, Vale et al 2014. ABA has been widely used in maturation medium because it can induce the final development of the embryo through the accumulation of LEA proteins (late embryogenesis abundant), related to the maintenance of desiccation tolerance (Dodeman et al 1997, Capelle et al 2010, stimulate the storage of reserve substances (Gutmann et al 1996, Sholi et al 2009) and prevent precocious germination (von Arnold et al 2002).…”

Section: Maturation Of Somatic Embryosmentioning

confidence: 99%

“…The genotype may influence the variation of embryogenic responses during the induction and maturation phase in plant tissue (Bell et al 1993). In addition, factors such as the type and concentration of auxin (Anandan et al 2012), the type of explant (Capelo et al 2011), presence or absence of abscisic acid (Bell et al 1993), activated carbon (López-Pérez et al 2005) or osmotic substances (Heringer et al 2013) can alter the responses among genotypes.…”

Section: Introductionmentioning

confidence: 99%

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Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Cipriano

Cruz

Mancini

et al. 2018

An. Acad. Bras. Ciênc.

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The aim of this study was to evaluate somatic embryogenesis in juvenile explants of the THB papaya cultivar. Apical shoots and cotyledonary leaves were inoculated in an induction medium composed of different concentrations of 2,4-D (6, 9, 12, 15 and 18 µM) or 4-CPA (19, 22, 25, 28 and 31 µM). The embryogenic calluses were transferred to a maturation medium for 30 days. Histological analysis were done during the induction and scanning electron microscopy after maturing. For both types of auxin, embryogenesis was achieved at higher frequencies with cotyledonary leaves incubated in induction medium than with apical shoots; except for callogenesis. The early-stage embryos (e.g., globular or heartshape) predominated. Among the auxins, best results were observed in cotyledonary leaves induced with 4-CPA (25 µM). Histological analyses of the cotyledonary leaf-derived calluses confirmed that the somatic embryos (SEs) formed from parenchyma cells, predominantly differentiated via indirect and multicellular origin and infrequently via synchronized embryogenesis. The secondary embryogenesis was observed during induction and maturation phases in papaya THB cultivar. The combination of ABA (0.5 µM) and AC (15 g L -1 ) in maturation medium resulted in the highest somatic embryogenesis induction frequency (70 SEs callus -1 ) and the lowest percentage of early germination (4%).

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Section: Maturation Of Somatic Embryosmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Cipriano

Cruz

Mancini

et al. 2018

An. Acad. Bras. Ciênc.

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“…This approach has also been used in our research on both spruce species, but its effectiveness was found to differ between ET lines (Hazubska & Szczygieł, 2003). Many studies have indicated that PEG is an inducer of both somatic embryo maturation and the conversion of somatic embryos into functional seedlings (Heringer et al, 2013). Supplementing a culture medium with PEG, a non-permeating osmoticum, simulates the water stress that occurs during normal seed development and this is believed to affect somatic embryo maturation (Heringer et al, 2013;Koehler et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Osmotic stress in cultures of conifer tissues can be induced by the use of several compounds, such as amino acids, mannitol, or dextran. The addition of polyethylene glycol (PEG) to the maturation medium, however, has become the most common method used to stimulate embryo maturation (Belmonte et al, 2005;Heringer et al, 2013;Hudec et al, 2016). PEG is a non-permeating osmoticum that increases desiccation tolerance and induces the deposition of storage proteins in cells (Attree et al, 1991;Misra et al, 1993;Heringer et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…The addition of polyethylene glycol (PEG) to the maturation medium, however, has become the most common method used to stimulate embryo maturation (Belmonte et al, 2005;Heringer et al, 2013;Hudec et al, 2016). PEG is a non-permeating osmoticum that increases desiccation tolerance and induces the deposition of storage proteins in cells (Attree et al, 1991;Misra et al, 1993;Heringer et al, 2013). The positive effect of PEG on embryo maturation has been reported for many coniferous species, including Picea abies (Hudec et al, 2016), P. likiangensis (Chen et al, 2010), P. glauca (Stasolla et al, 2002;Stasolla & Yeung, 2003), and Cryptomeria japonica (Maruyama et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Stimulation of somatic embryo growth and development in Picea spp. by polyethylene glycol

Hazubska-Przybył¹,

Wawrzyniak²

2017

Dendrobiology

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Abstract:The objective of the presented study was to compare the effect of osmotic stress, induced by various concentrations of polyethylene glycol (PEG) MW 4000, on the development, maturation, and conversion of somatic embryos of Picea abies (model species) and P. omorika (endemic species) into somatic seedlings. Embryogenic tissue (ET) of P. abies (line A and B) and P. omorika were incubated on a maturation medium with 20 µM abscisic acid (ABA), 1 µM indole-3-butyric acid (IBA) and PEG 4000 at 0, 5, and 7.5%. After 5 weeks, the number of somatic embryos produced, and the number of cotyledonary somatic embryos per 1 gram of ET, was assessed. Cotyledonary somatic embryos were selected and transferred to a Margara germination medium for two months. Their growth, as measured by hypocotyl and radicle length, was evaluated after one month, and the somatic embryos were transferred to fresh medium. After the second month of incubation, the conversion frequency of germinated embryos into the seedlings was assessed. In general, ETs of both of the tested spruce species cultured on a maturation medium amended with 5% PEG 4000 exhibited a tendency to produce a higher total number of embryos, as well as a higher number of cotyledonary embryos. The B line of P. abies produced a significantly greater number of cotyledonary embryos, relative to the control (no PEG). PEG only stimulated hypocotyl growth in embryos of both lines (A and B) of P. abies during the germination stage. Somatic embryos of Picea omorika were characterized by poor growth of both hypocotyls and radicles, irrespective of PEG treatment. The addition of PEG 4000 to the maturation medium did not improve the conversion frequency of germinated somatic embryos into somatic seedlings in either spruce species after a month of incubation on Margara medium. The effect of PEG 4000 on somatic embryogenesis efficiency was more evident in the ET of the two P. abies lines, relative to the P. omorika ET line, during both the maturation and the germination stage. Results indicated that the P. omorika ET line was almost completely insensitive to all of the PEG concentrations tested.

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In Vitro Stress-Mediated Somatic Embryogenesis in Plants

Spinoso-Castillo

Bello-Bello

2022

Methods in Molecular Biology

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Polyethylene glycol effects on somatic embryogenesis of papaya hybrid UENF/CALIMAN 01 seeds

Cited by 26 publications

References 38 publications

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Stimulation of somatic embryo growth and development in Picea spp. by polyethylene glycol

In Vitro Stress-Mediated Somatic Embryogenesis in Plants

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