Polyethylene glycol gold-nanoparticles: Facile nanostructuration of doxorubicin and its complex with DNA molecules for SERS detection

Spadavecchia, Jolanda; Perumal, Ramesh Kannan; Casale, Sandra; Krafft, Jean‐Marc; Méthivier, Christophe; Pradier, Claire‐Marie

doi:10.1016/j.cplett.2015.08.038

Cited by 15 publications

(9 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SERS studies of DNA damage primarily focus on the interaction of chemotherapeutic drugs with the DNA molecule. The impact of doxorubicin on oligonucleotide structure was studied by Spadavecchia et al, using pegylated gold nanoparticles (PEG-AuNPs) covalently linked to the cysteamine-gold surface via amide bond, as a SERS substrate [100]. SERS band at 1591 cm −1 confirms the formation of the DNA-doxorubicin complex.…”

Section: Sersmentioning

confidence: 99%

“…The SERS bands characteristic for doxorubicin-DNA interaction are presented in Table 2. The doxorubicin interaction with dsDNA was studied in various SERS configurations, using different SERS enhancers [100,[107][108][109], which resulted in a shift in the spectral position of characteristic bands, or a change in their intensity. In many cases, the bands corresponding to the in-plane bending of hydroxyl groups at doxorubicin rings, usually found around 1210 and 1244 cm −1 , were very weak or absent in the SERS spectrum, owing to drug intercalation into dsDNA structure [101,107].…”

Section: Sersmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Spectroscopic Markers of DNA Damage

Sofińska¹,

Wilkosz²,

Szymoński³

et al. 2020

Molecules

View full text Add to dashboard Cite

Every cell in a living organism is constantly exposed to physical and chemical factors which damage the molecular structure of proteins, lipids, and nucleic acids. Cellular DNA lesions are the most dangerous because the genetic information, critical for the identity and function of each eukaryotic cell, is stored in the DNA. In this review, we describe spectroscopic markers of DNA damage, which can be detected by infrared, Raman, surface-enhanced Raman, and tip-enhanced Raman spectroscopies, using data acquired from DNA solutions and mammalian cells. Various physical and chemical DNA damaging factors are taken into consideration, including ionizing and non-ionizing radiation, chemicals, and chemotherapeutic compounds. All major spectral markers of DNA damage are presented in several tables, to give the reader a possibility of fast identification of the spectral signature related to a particular type of DNA damage.

show abstract

Section: Sersmentioning

confidence: 99%

Section: Sersmentioning

confidence: 99%

Molecular Spectroscopic Markers of DNA Damage

Sofińska¹,

Wilkosz²,

Szymoński³

et al. 2020

Molecules

View full text Add to dashboard Cite

show abstract

“…In particular, S.Yang et al have indicated that citric acid makes complexes with Au(III) at several pH values, affecting the particle size . In this case, PEG diacide, about citric acid, intervenes the formation of complex PEG–Au(III)–Drug for the growth of NPs. ,, …”

Section: Resultsmentioning

confidence: 99%

Lenalidomide (LENA) Hybrid Gold Complex Nanoparticles: Synthesis, Physicochemical Evaluation, and Perspectives in Nanomedicine

Arib

Spadavecchia

2020

ACS Omega

Self Cite

View full text Add to dashboard Cite

This paper proposes a fast methodology to synthesize hybrid lenalidomide gold nanoparticles. Gold (HAuCl4) is chelated with an antiangiogenic compound (lenalidomide (LENA)) and diacid poly(ethylene glycol) (PEG) as capping agent and reagent. The suggested synthesis is rapid and results in gold nanoparticles (AuNPs) with enhanced drug solubility. The binding between LENA, PEG, and Au(III) ions forms hybrid nanovectors named LENA IN PEG-AuNPs, which were characterized by different spectroscopic techniques (Raman and UV–vis), transmission electron microscopy (TEM), and compared with LENA ON PEG-AuNPs, in which the drug was grafted onto gold surface by carbodiimide chemistry (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide, EDC/NHS). The effective drug delivery under pH conditions was also reached, combined with doxorubicin (DOX) to improve the synergic chemotherapy and stability under experimental conditions. For biomedical purposes, hybrid gold nanocarriers were conjugated with folic acid (FA), which is specifically overexpressed in cancer cells. This paper will be very important in the domain of therapeutic gold complex, paving the way for reaching progress of novel drug carrier synthesis in nanomedicine.

show abstract

“…The decrease in intensity of the bands at 1214 and 1246 cm −1 assigned to in-plane C-O, C-O-H vibrations and C-H bending confirms the intercalation of doxorubicin rings within the DNA structure. The studies on DNA-doxorubicin binding process using synthesized nanoscale particles with probe/target oligonucleotides as a SERS substrate were continued by Spadavecchia et al [93]. Presence of the doxorubicin-DNA complex was confirmed by appearance of the band at 1591 cm −1 in the SERS spectrum.…”

Section: Protective Role Of Solventsmentioning

confidence: 98%

Revealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy

et al. 2021

View full text Add to dashboard Cite

DNA covers the genetic information in all living organisms. Numerous intrinsic and extrinsic factors may influence the local structure of the DNA molecule or compromise its integrity. Detailed understanding of structural modifications of DNA resulting from interactions with other molecules and surrounding environment is of central importance for the future development of medicine and pharmacology. In this paper, we review the recent achievements in research on DNA structure at nanoscale. In particular, we focused on the molecular structure of DNA revealed by high-resolution AFM (Atomic Force Microscopy) imaging at liquid/solid interfaces. Such detailed structural studies were driven by the technical developments made in SPM (Scanning Probe Microscopy) techniques. Therefore, we describe here the working principles of AFM modes allowing high-resolution visualization of DNA structure under native (liquid) environment. While AFM provides well-resolved structure of molecules at nanoscale, it does not reveal the chemical structure and composition of studied samples. The simultaneous information combining the structural and chemical details of studied analyte allows achieve a comprehensive picture of investigated phenomenon. Therefore, we also summarize recent molecular spectroscopy studies, including Tip-Enhanced Raman Spectroscopy (TERS), on the DNA structure and its structural rearrangements.

show abstract

Polyethylene glycol gold-nanoparticles: Facile nanostructuration of doxorubicin and its complex with DNA molecules for SERS detection

Cited by 15 publications

References 41 publications

Molecular Spectroscopic Markers of DNA Damage

Molecular Spectroscopic Markers of DNA Damage

Lenalidomide (LENA) Hybrid Gold Complex Nanoparticles: Synthesis, Physicochemical Evaluation, and Perspectives in Nanomedicine

Revealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy

Contact Info

Product

Resources

About