Polyethylene-glycol-modified zwitterionic polymer assisted protein aggregation arrest and refolding

Abstract

Protein aggregation limits the development of protein-based drugs, and leads to neurological disorders. In this study, the zwitterionic polymer, poly-sulfobetaine (p-SPB), was modified using the polyethylene glycol (PEG), and exhibited...

Polyampholyte‐Based Polymer Hydrogels for the Long‐Term Storage, Protection and Delivery of Therapeutic Proteins

“…Previous studies have reported that PEG facilitates the suppression of protein aggregation owing to its ability to strongly interact with thermally unfolded proteins, thus protecting them from aggregation-inducing collisions. [31,42] Hence, a synergistic effect is observed where PEG and PLLSA work together to realize higher activity than those of both the individual components. The secondary structure of in-sulin was retained, and the CD spectra of the insulin combined with micelles were comparable to those of the native protein (Figure S21, Supporting Information).…”

Design of self-assembled glycopolymeric zwitterionic micelles as removable protein stabilizing agents

“…In our previous studies, we showed that poly-sulfobetaine (poly-SPB), a zwitterionic polymer, exhibits good efficiency in protecting proteins from thermal denaturation. [19][20][21][22][23][24] A number of poly-SPB derivatives have been prepared by adding hydrophobic monomers, transforming into nanogels, or preparing gra polymers to achieve higher activity. Although previously known agents and zwitterionic polymers have remarkable abilities to protect proteins, a major drawback of this process is the inability to separate proteins from the stabilizing agents.…”

Polyampholytes and Their Hydrophobic Derivatives as Excipients for Suppressing Protein Aggregation

“…Many classes of small molecules, such as polyamines, vitamins, polyphenols, peptides, and nondetergent sulfobetaines, have been successfully applied for this purpose. Additionally, a few polymers with relatively good performance have recently been reported. − In particular, zwitterionic polymers have been shown to inhibit the aggregation of lysozyme under thermal stress and facilitate the retention of enzymatic activity. − However, very high concentrations of zwitterionic polymers are required to achieve good efficiency, which is not feasible for industrial or clinical applications. , Hence, for applications in protein processing and protein therapeutics, it is highly desirable to develop polymeric systems with significantly improved efficacy.…”