Polyethylene glycol triggers the anti-cancer impact of curcumin nanoparticles in sw-1736 thyroid cancer cells

Abstract

Curcumin has been recognized as an effective anticancer agent. However, due to its hydrophobic property, the cell absorption is not satisfied. Herein, the curcumin nanoparticles were prepared in the presence of polyethylene glycol 6000 (PEG6000) to reduce its elimination by immune system. For first time, not only the curcumin was encapsulated within the niosome nanoparticles modified by PEG, there are no reports related to the anticancer property of curcumin against thyroid cancers. The nanoparticles was developed and its anticancer was studied on sw-1736 cancer cell line. The nanoparticles were examined by scanning electron microscopy (SEM) and dynamic light scattering (DLS). Also, the release profile of curcumin, the IC50 concentration, the radical amount and the gene expression were evaluated. The optimized nanoparticles showed a diameter of 212 ± 31 nm by SEM and the encapsulation efficiency and loading capacity of 76% and 16.8% respectively. DLS confirmed the polydispersity index (PDI) of 0.596 and the release model was shown a sustained release with the delivery of 68% curcumin after 6 days. Also, the nanoparticles indicated the higher storage stability at 4 °C. After the cell treatment, the apoptotic bodies were appeared and IC50 was obtained as 0.159 mM. Moreover, the generated radicals by the treated cells was 86% after 72 h and the gene pattern indicated the bax/bcl2 ratio of 6.83 confirming the apoptosis effect of the nanoparticles. The results approved the nanoparticles could be suggested as an anticancer drug candidate for thyroid cancers.

Controlled Release of Curcumin and Hypocrellin A from Electrospun Poly(l-Lactic Acid)/Silk Fibroin Nanofibers for Enhanced Cancer Cell Inhibition

“…Other research has demonstrated that HA can generate a large amount of singlet oxygen and induce apoptosis in cancer cells. 67 In our study, after incubation with drug-loaded nanofiber membranes for 24 h, the nuclei were shrunken and fragmented, and the cytoskeleton became incomplete, indicating apoptosis. Similar to these findings, nanofibers loaded with HA and curcumin showed differences in cell morphology, confirming that drugloaded nanofibers can serve as effective carriers for cancer treatment.…”

“…Studies on SW-1736 thyroid cancer cells cocultured with curcumin-liposome nanoparticles for 72 h also showed significant changes in cell morphology. Other research has demonstrated that HA can generate a large amount of singlet oxygen and induce apoptosis in cancer cells . In our study, after incubation with drug-loaded nanofiber membranes for 24 h, the nuclei were shrunken and fragmented, and the cytoskeleton became incomplete, indicating apoptosis.…”

Multifaceted physiological and therapeutical impact of curcumin on hormone‐related endocrine dysfunctions: A comprehensive review

“…To address the bioavailability challenges associated with Cur, a study developed CUR‐NPs and investigated their anticancer properties against thyroid cancers. The NPs exhibited enhanced cellular uptake, radical generation, and apoptotic effects on sw‐1736 cancer cells, suggesting their potential as an anticancer drug candidate for thyroid cancers (Hosseinzadeh et al, 2021). Combination therapies involving Cur were also explored in the context of thyroid cancer.…”

Nanomaterials: a promising multimodal theranostics platform for thyroid cancer

“…Curcumin nanoparticles promoted the production of free radicals in anaplastic thyroid carcinoma (ATC) cells, leading to cell apoptosis through the expression of Bax, caspase-3, and caspase-8 gene pathways. 143 Nanomaterials carrying natural anti-tumor agents have the potential to achieve a stronger anti-tumor effect than chemotherapy drugs, with fewer toxic side effects. Resveratrol, a nonflavonoid polyphenol organic compound found in grape leaves and skins, has demonstrated antioxidant, anti-inflammatory, anticancer, and cardiovascular protective effects in vitro and animal experiments.…”

“…Curcumin nanoparticles promoted the production of free radicals in anaplastic thyroid carcinoma (ATC) cells, leading to cell apoptosis through the expression of Bax, caspase-3, and caspase-8 gene pathways. 143…”