Polyethylenimine-coated PLGA nanoparticles-encapsulated Angelica sinensis polysaccharide as an adjuvant to enhance immune responses

Gu, Peng; Wusiman, Adelijiang; Wang, Siyuan; Zhang, Yue; Liu, Zhenguang; Hu, Yuanliang; Liu, Jiaguo; Wang, Deyun

doi:10.1016/j.carbpol.2019.115128

Cited by 105 publications

(57 citation statements)

References 54 publications

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“…Many polysaccharides from plant, bacterial, yeast and synthetic sources can act as pathogen-associated molecular patterns (PAMPs) and recognize pattern recognition receptors (PRRs) on immune cells, followed by regulating immunity [6][7][8][9]. Polysaccharides are safe, and biodegradable, with no tissue deposition [10].…”

Section: Introductionmentioning

confidence: 99%

Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner

et al. 2020

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There is an urgent need for the development of new, improved vaccine adjuvants against T. spiralis infection. Polysaccharides are effective, safe, and biodegradable as adjuvant. In our study, we first observed the protective efficacy of lentinan as adjuvant against helminth T. spiralis infection. Recombinant T. spiralis Serpin (rTs-Serpin) immunoscreened from a cDNA library of T. spiralis, as a vaccine, protect host against Trichinella infection. The reduction rate of helminth burden of rTs-Serpin+lentinan-immunized mice was significantly increased compared with rTs-Serpin+FCA-immunized mice. rTs-Serpin+lentinan induced IgG1-dominant immune response and higher levels of IFN-γ and IL-4. rTs-Serpin+lentinan displayed a lower reduction rate of parasite burden in NLRP3-/mice than that in WT mice and lower level of IgG1 than that in WT mice. The level of IL-4, but not IFN-γ, from NLRP3-/mice immunized by rTs-Serpin+lentinan was significantly lower than that from WT mice, suggesting that NLRP3 is associated with rTs-Serpin+lentinan-triggering Th2 protective immunity against T. spiralis infection. In summary, we revealed that lentinan was a novel adjuvant against T. spiralis infection via NLRP3. NLRP3 therefore represents an important target for adjuvant discovery and the control of T. spiralis infection.

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Section: Introductionmentioning

confidence: 99%

Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner

et al. 2020

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“…Adjuvant is an essential component used in vaccine designing to boost the immune response with minimum amount of antigen, to regulate the immunogenicity and to offers better protection by mean of long-period impact of vaccine [ [116] , [117] , [118] ]. More specifically, the use of adjuvant manages (i) the vaccine dosages (ii) promotes slow release of antigen, (iii) retains antigenecity of antigen for longer time and (iv) activate selective pathways of immunity against vaccine antigen [ [116] , [117] , [118] ]. The actual mechanism of the adjuvant functioning is not well known, but its use offers more benefits for effective functioning of vaccine [ [116] , [117] , [118] ].…”

Section: Other Potential Factors Responsible For the Development Of Cmentioning

confidence: 99%

“…More specifically, the use of adjuvant manages (i) the vaccine dosages (ii) promotes slow release of antigen, (iii) retains antigenecity of antigen for longer time and (iv) activate selective pathways of immunity against vaccine antigen [ [116] , [117] , [118] ]. The actual mechanism of the adjuvant functioning is not well known, but its use offers more benefits for effective functioning of vaccine [ [116] , [117] , [118] ]. Various kinds of the adjuvants are reported in the literatures ( Table 6 , entries 1–10) that are used in coronavirus vaccine development.…”

Section: Other Potential Factors Responsible For the Development Of Cmentioning

confidence: 99%

“…The uses of the adjuvant make it possible to immunize to more number of people as it facilitates the requirement of small dosages [ 116 , 117 ]. However in case of respiratory disease related vaccine, choice of adjuvant plays an important role, since some nano-particles, lipid and inulin based adjuvants causes toxicity to the lung tissue which are rich in immune cells and macrophages [ [119] , [120] , [121] ].…”

Section: Other Potential Factors Responsible For the Development Of Cmentioning

confidence: 99%

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Vaccine development against coronavirus (2003 to present): An overview, recent advances, current scenario, opportunities and challenges

Badgujar¹,

Badgujar

Badgujar³

2020

Diabetes & Metabolic Syndrome: Clinical Research & Revi

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Background and aim The pandemic COVID-19 occurring due to novel emerging coronavirus-2019 (SARS-CoV-2) is severely affecting the worldwide public health, culture, economy and human social behaviour. Till date, there is no approved medicine/treatment to cure COVID-19, whereas, vaccine development efforts are going on high priority. This review aimed to provide an overview of prior art, recent advances, vaccine designing strategies, current scenario, opportunities and challenges related to development of coronavirus vaccine. Method A literature survey was conducted using Scopus, PubMed and Google Scholar with the search key as: coronavirus vaccine, SARS vaccine, MERS vaccine and COVID-19 vaccine. Articles related to above search query were retrieved, sorted, analyzed and developed into an easy-to-understand review. Results The genome phylogenetic analysis suggested that genomic sequence of SARS-CoV-2 is almost 80% similar to that of SARS-CoV, further both these viruses bind to same host cell receptor ACE-2. Hence it is expected that, previously available literature data about coronavirus vaccine designing may play crucial role in development of rapid vaccine against COVID-19. In view of this, the present review discuss (i) existing information (from 2003 to present) about the type of vaccine, antigen, immunogenic response, animal model, route of administration, adjuvants and current scenario for designing of coronavirus vaccine (ii) potential factors and challenges related to rapid development of COVID-19 vaccine. Conclusion In conclusion, we discuss possible clues/ target sites for designing of vaccine against SARS-CoV-2 virus based on prior-art.

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“…19 However, the negative charge of unmodified PLNPs often limits their adjuvant activity. 20 As a widely used cationic polymer, polyethyleneimine (PEI) has been used to modify the surface charge of PLNPs. 21,22 Therefore, in this study, we used PLNPs to load the PPARγ agonist RSG and prepare PLGA-RSG nanoparticles (PLNPs-RSG).…”

Section: Introductionmentioning

confidence: 99%

<p>Ultrasound Assisted a Peroxisome Proliferator-Activated Receptor (PPAR)γ Agonist-Loaded Nanoparticle-Microbubble Complex to Attenuate Renal Interstitial Fibrosis</p>

Wei

Zhang

et al. 2020

IJN

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Objective: To investigate the antifibrotic effect of the combination of a PPARγ agonistloaded nanoparticle-microbubble complex with ultrasound (US) exposure on renal interstitial fibrosis (RIF). Materials and Methods: Polylactide-co-glycolide (PLGA) nanoparticles were used to load PPARγ agonist (rosiglitazone, RSG) and prepare PLGA-RSG nanoparticles (PLNPs-RSG); then, a novel complex between PLNPs-RSG and SonoVue microbubbles (MBs) (PLNPs-RSG-MBs) was prepared. The size distribution, zeta potentials, RSG-loading capacity and entrapment efficiency were measured, and the release of RSG was assessed using a UV-vis spectrophotometer. The in vitro cytotoxicity and in vivo systemic toxicity assays were performed. The cellular uptake assessment was performed using a confocal laser scanning microscope (CLSM). The in vivo biodistribution assessment was performed using fluorescence imaging with a near-infrared (NIR) imaging system. Furthermore, this complex was administered to a unilateral ureteral obstruction (UUO) rat model with the assistance of US exposure to investigate the antifibrotic effect. Results: This PLNPs-RSG-MBs complex had a size of 2199.5± 988.1 nm and a drugloading efficiency of 28.5%. In vitro cytotoxicity and in vivo systemic toxicity assays indicated that the PLNPs-RSG-MBs complex displayed excellent biocompatibility. In addition, the complex showed high cellular uptake efficiency in vitro and kidney-targeting ability in vivo. In a UUO rat model, the combination of the PLNPs-RSG-MBs complex with US exposure significantly reduced collagen deposition and successfully attenuated renal fibrosis. Conclusion: The combination of the PLNPs-RSG-MBs complex with US exposure may be a promising approach for the treatment of RIF.

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Polyethylenimine-coated PLGA nanoparticles-encapsulated Angelica sinensis polysaccharide as an adjuvant to enhance immune responses

Cited by 105 publications

References 54 publications

Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner

Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner

Vaccine development against coronavirus (2003 to present): An overview, recent advances, current scenario, opportunities and challenges

<p>Ultrasound Assisted a Peroxisome Proliferator-Activated Receptor (PPAR)γ Agonist-Loaded Nanoparticle-Microbubble Complex to Attenuate Renal Interstitial Fibrosis</p>

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