Polyethylenimine-coated SPION exhibits potential intrinsic anti-metastatic properties inhibiting migration and invasion of pancreatic tumor cells

Mulens-Arias, Vladimir; Rojas, José M.; Pérez-Yagüe, Sonia; Morales, M.P.; Barber, Domingo F.

doi:10.1016/j.jconrel.2015.08.009

Cited by 29 publications

(28 citation statements)

References 79 publications

(100 reference statements)

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“…Moreover, Perillo and colleagues showed that gH625-PEG-IONs have high cytoplasmic distribution, making them good candidates for cancer theranostic treatments [ 161 ]. BUF II-conjugated IONs have also demonstrated great translocating abilities due to the high arginine content of BUF II, as we have shown in previous studies [ 131 , 132 ]. ION-PEA-BUF II and Ag/ION-pDMAEMA/PEA-BUF II nanovehicles showed homogeneous cytosolic distribution after 1 h of incubation with THP-1 and neuroblastoma (SH-SY5Y) cells, respectively, and only 27% and 24% colocalized with endosomal compartments after this time period [ 131 , 132 ].…”

Section: Enhancing Ion Endosomal Escapesupporting

confidence: 67%

“…This approach was explored by Song and colleagues through novel PolyMag/DNA/Tat-peptide nanoparticles with endosomal escape abilities and a 4-fold improvement in transfection over the complexes without the peptide [ 253 ]. The same capability has also been shown for ION-PEA-BUF-II nanobioconjugates developed by our group, which were able to efficiently penetrate several mammalian cell lines without significant impact on viability and presented an overall homogeneous cytosolic distribution [ 131 , 254 ]. In addition to agents suitable for protonation, Cristofolini and colleagues developed a novel vehicle that induces the proton-sponge-effect by increasing the luminal concentration of cationic molecules to form an internal hypertonic medium.…”

Section: Enhancing Ion Endosomal Escapesupporting

confidence: 63%

“…Previous work from our group also demonstrated the nuclear penetration of whole PEA-ION nanobioconjugates modified with the peptide Buforin II (i.e., BUF-II-PEA-IONs). This activity was attributed to the presence of BUFII and the ability of the PEA surface extensor to maintain BUF-II’s cell-penetrating capabilities [ 131 ].…”

Section: An Overview Of Ion-mediated Transfection In Gene Editingmentioning

confidence: 99%

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Tailoring Iron Oxide Nanoparticles for Efficient Cellular Internalization and Endosomal Escape

et al. 2020

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Iron oxide nanoparticles (IONs) have been widely explored for biomedical applications due to their high biocompatibility, surface-coating versatility, and superparamagnetic properties. Upon exposure to an external magnetic field, IONs can be precisely directed to a region of interest and serve as exceptional delivery vehicles and cellular markers. However, the design of nanocarriers that achieve an efficient endocytic uptake, escape lysosomal degradation, and perform precise intracellular functions is still a challenge for their application in translational medicine. This review highlights several aspects that mediate the activation of the endosomal pathways, as well as the different properties that govern endosomal escape and nuclear transfection of magnetic IONs. In particular, we review a variety of ION surface modification alternatives that have emerged for facilitating their endocytic uptake and their timely escape from endosomes, with special emphasis on how these can be manipulated for the rational design of cell-penetrating vehicles. Moreover, additional modifications for enhancing nuclear transfection are also included in the design of therapeutic vehicles that must overcome this barrier. Understanding these mechanisms opens new perspectives in the strategic development of vehicles for cell tracking, cell imaging and the targeted intracellular delivery of drugs and gene therapy sequences and vectors.

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Section: Enhancing Ion Endosomal Escapesupporting

confidence: 67%

Section: Enhancing Ion Endosomal Escapesupporting

confidence: 63%

Section: An Overview Of Ion-mediated Transfection In Gene Editingmentioning

confidence: 99%

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Tailoring Iron Oxide Nanoparticles for Efficient Cellular Internalization and Endosomal Escape

et al. 2020

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“…AuNPs MAPK signalling Inhibited the proliferation of SKOV3 (ovarian) cancer cells and delayed the tumoral and metastases growth by reversing EMT and inhibition of MAPK signalling [27] PEI coated SPIONs Src kinase, miR-21, MMP2 Reduced the invadosome intensity and decreased the ability of Pan02 (pancreatic cancer) cells to invade through basement membrane. [28] FA-PEG-PEI-SPIONs miR-125b-5p JAK-STAT, Wnt/β-Catenin Inhibited the invasion, migration, and growth of HCC HUH7 and HCCLM3 cells [29] Zinc…”

Section: Invasion and Metastasismentioning

confidence: 99%

“…Polyethylenimine coated superparamagnetic iron NPs (SPIONs) are proven to inhibit tumour cell migration and invasion through the inhibition of Src kinase activity and downregulation of MT1-MMP and MMP2 matrix-metalloproteinases. In addition, SPIONs treatment can downregulate miR-21, upregulating cell migration inhibitors PTEN, PDCD4 and sproutyl-1 [28].…”

Section: Targeting Emt and Metastatic Progression With Nanoparticle Fmentioning

confidence: 99%

Exploiting Current Understanding of Hypoxia Mediated Tumour Progression for Nanotherapeutic Development

Feng

Byrne

Jamal

et al. 2019

Cancers

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Hypoxia is one of the most common phenotypes of malignant tumours. Hypoxia leads to the increased activity of hypoxia-inducible factors (HIFs), which regulate the expression of genes controlling a raft of pro-tumour phenotypes. These include maintenance of the cancer stem cell compartment, epithelial-mesenchymal transition (EMT), angiogenesis, immunosuppression, and metabolic reprogramming. Hypoxia can also contribute to the tumour progression in a HIF-independent manner via the activation of a complex signalling network pathway, including JAK-STAT, RhoA/ROCK, NF-κB and PI3/AKT. Recent studies suggest that nanotherapeutics offer a unique opportunity to target the hypoxic microenvironment, enhancing the therapeutic window of conventional therapeutics. In this review, we summarise recent advances in understanding the impact of hypoxia on tumour progression, while outlining possible nanotherapeutic approaches for overcoming hypoxia-mediated resistance.

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Carboxymethyl dextran‐trimethyl chitosan coated superparamagnetic iron oxide nanoparticles: An effective siRNA delivery system for HIV‐1 Nef

Kamalzare

Noormohammadi

Rahimi

et al. 2019

Journal Cellular Physiology

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Gene therapy, including small interfering RNA (siRNA) technology, is one of the leading strategies that help to improve the outcomes of the current therapeutic systems against HIV-1 infection. The successful therapeutic application of siRNAs requires their safe and efficient delivery to specific cells. Here, we introduce a superparamagnetic iron oxide nanoparticle (SPION) for delivering siRNA against HIV-1 nef (anti-nef siRNA) into two cell lines, HEK293 and macrophage RAW 264.7.SPIONs were coated with trimethyl chitosan (TMC), and thereafter, different concentrations of SPION-TMC were coated with different ratios of a carboxymethyl dextran (CMD) to modify the physicochemical properties and improve the biological properties of the nanocarriers. The nanoparticles exhibited a spherical shape with an average size of 112 nm. The obtained results showed that the designed delivery route enhanced the uptake of siRNA into both HEK293 and RAW 264.7 cells compared with control groups. Moreover, CMD-TMC-SPIONs containing anti-nef siRNA significantly reduced the expression of HIV-1 nef in HEK293 stable cells. The modified siRNA-loaded SPIONs also displayed no toxicity or apoptosis-inducing

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Polyethylenimine-coated SPION exhibits potential intrinsic anti-metastatic properties inhibiting migration and invasion of pancreatic tumor cells

Cited by 29 publications

References 79 publications

Tailoring Iron Oxide Nanoparticles for Efficient Cellular Internalization and Endosomal Escape

Tailoring Iron Oxide Nanoparticles for Efficient Cellular Internalization and Endosomal Escape

Exploiting Current Understanding of Hypoxia Mediated Tumour Progression for Nanotherapeutic Development

Carboxymethyl dextran‐trimethyl chitosan coated superparamagnetic iron oxide nanoparticles: An effective siRNA delivery system for HIV‐1 Nef

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