Polyethylenimine-mediated gene transfer into pancreatic tumor dissemination in the murine peritoneal cavity

Aoki, Kazunori; Furuhata, Souichi; Hatanaka, Kazuteru; Maeda, Miho; Js, Remy; Jp, Behr; Terada, Masaaki; Yoshida, Teruhiko

doi:10.1038/sj.gt.3301435

Cited by 118 publications

(103 citation statements)

References 31 publications

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“…DNA (1 mg) was subjected to PCR analysis using the primers previously described by Aoki et al 2 (Luci L, 5 0 -GCGCC ATTCTATCCGCTGGA-3 0 ; Luci R, 5 0 -CTATCGAAG GACTCTGGCAC-3 0 ) and amplified under the following cycling conditions : preincubation at 941C for 5 min, then 941C for 1 min, 601C for 30 s and 721C for 2 min for 40 cycles and postincubation at 721C for 3 min. The PCR products were visualized on agar gel.…”

Section: Luminometrymentioning

confidence: 99%

Intraperitoneal linear polyethylenimine (L-PEI)-mediated gene delivery to ovarian carcinoma nodes in mice

Dutoit

Denoux

et al. 2005

Cancer Gene Ther

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Linear polyethylenimine (L-PEI) is an efficient transfection agent for ovarian carcinoma cells in vitro and ex vivo. In the present work, we go a step further and evaluate the efficacy of L-PEI in human ovarian tumor nodes developed in mice. PEI/DNA complexes were administered intraperitoneally instead of intravenously to avoid sequestering of complexes in the lung and liver and to allow transfection of nonvascularized tumor nodes. Plasmid biodistribution was studied by PCR and gene expression was characterized using complementary luciferase and b-galactosidase assays. Intraperitoneal (i.p.) injection of L-PEI/DNA complexes allowed the straightforward distribution of plasmid in the whole peritoneal cavity. Gene expression occurred in many organs, but tumor nodes appeared as preferential sites for transgene expression. The i.p. delivery route allowed repeated injections and administration of large amounts of DNA (up to 400 mg) without signs of toxicity, even for doses well beyond the intravenous lethal dose. Transgene expression was dose-dependent and transient. However, multiple injections allowed its persistence to increase. These results provide encouraging elements towards the development of PEI-based gene therapy protocols for the treatment of advanced stage ovarian carcinoma.

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Section: Luminometrymentioning

confidence: 99%

Intraperitoneal linear polyethylenimine (L-PEI)-mediated gene delivery to ovarian carcinoma nodes in mice

Dutoit

Denoux

et al. 2005

Cancer Gene Ther

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“…34 Only a small number of scattered blue cells were observed. Although the transfection efficiency mediated by intraperitoneal gene delivery is relatively low, genes encoding a secreted protein with therapeutic potential may overcome this handicap using a paracrine effect, secreting adequate protein to reach physiologically meaningful levels locally.…”

Section: Discussionmentioning

confidence: 96%

Intraperitoneal gene delivery mediated by a novel cationic liposome in a peritoneal disseminated ovarian cancer model

et al. 2002

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“…X-gal and AP staining was performed as described. 47,49 (e) RT-PCR analysis of organ distribution of lacZ gene expression 2 days after the intravenous injection of AxCA-lacZ. The untreated normal liver did not show any PCR band.…”

Section: Intravenous Adenovirus Vector Injection Into Rats With Dmn-imentioning

confidence: 99%

Adenovirus-mediated gene transfer of interferon α improves dimethylnitrosamine-induced liver cirrhosis in rat model

Suzuki

Aoki²,

Ohnami³

et al. 2003

Gene Ther

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Polyethylenimine-mediated gene transfer into pancreatic tumor dissemination in the murine peritoneal cavity

Cited by 118 publications

References 31 publications

Intraperitoneal linear polyethylenimine (L-PEI)-mediated gene delivery to ovarian carcinoma nodes in mice

Intraperitoneal linear polyethylenimine (L-PEI)-mediated gene delivery to ovarian carcinoma nodes in mice

Intraperitoneal gene delivery mediated by a novel cationic liposome in a peritoneal disseminated ovarian cancer model

Adenovirus-mediated gene transfer of interferon α improves dimethylnitrosamine-induced liver cirrhosis in rat model

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