Polyethylenimine with acid-labile linkages as a biodegradable gene carrier

Kim, Young Heui; Park, Jeong Hyun; Lee, Minhyung; Kim, Yong Hee; Park, Tae Gwan; Kim, Sung Wan

doi:10.1016/j.jconrel.2004.11.008

Cited by 313 publications

(209 citation statements)

References 18 publications

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“…Degradable random polymers based on low molecular weight oligoethylenimine 800 Da (OEI) were previously described by several researchers including our group. 9,13,16,[28][29][30] In the current work, we designed better defined pseudodendritic polymers of low molecular weight starting with OEI as core building block, using different dioldiacrylates, which contain degradable ester bonds to form pseudodendritic branches and OEI acrylate ester-based pseudodendrimers for in vitro and in vivo gene transfer V Russ et al added various oligoamines to modify the pseudodendritic cores.…”

Section: Discussionmentioning

confidence: 99%

Novel degradable oligoethylenimine acrylate ester-based pseudodendrimers for in vitro and in vivo gene transfer

et al. 2007

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Section: Discussionmentioning

confidence: 99%

Novel degradable oligoethylenimine acrylate ester-based pseudodendrimers for in vitro and in vivo gene transfer

et al. 2007

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“…Polymer composed of PEI1.8K bonded with acidic bonds can show comparable transfection efficiency compared to high molecular weight PEIs such as PEI25K. Additionally, low endosomal pH (~ 4–5) can break down labile acid PEI to low molecular weight counterparts and reduces the cytotoxicity [73]. Many strategies, such as PEGylation, bonding PEI with functional groups and synthesis of biodegradable PEIs through crosslinking or grafting, have been introduced to reduce cytotoxicity and promote the transfection efficiency of PEI [74].…”

Section: Cellular Toxicity and Transfection Ability Of Peimentioning

confidence: 99%

Polyethylenimine-based nanocarriers in co-delivery of drug and gene: a developing horizon

Zakeri

Kouhbanani

Beheshtkhoo

et al. 2018

Nano Reviews & Experiments

234

150

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The meaning of gene therapy is the delivery of DNA or RNA to cells for the treatment or prevention of genetic disorders. The success rate of gene therapy depends on the progression and safe gene delivery system. The vectors available for gene therapy are divided into viral and non-viral systems. Viral vectors cause higher transmission efficiency and long gene expression, but they have major problems, such as immunogenicity, carcinogenicity, the inability to transfer large size genes and high costs. Non-viral gene transfer vectors have attracted more attention because they exhibit less toxicity and the ability to transfer large size genes. However, the clinical application of non-viral methods still faces some limitations, including low transmission efficiency and poor gene expression. In recent years, numerous methods and gene-carriers have been developed to improve gene transfer efficiency. The use of Polyethylenimine (PEI) based transfer of collaboration may create a new way of treating diseases and the combination of chemotherapy and gene therapy. The purpose of this paper is to introduce the PEI as an appropriate vector for the effective gene delivery.

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“…Cetyl trimethyl ammonium bromide (CTAB), as a cationic surfactant, has been applied to coat PLGA cationic particles for efficient DNA vaccine delivery [8,9]. Cationic polymers, including polyethyleneimine (PEI) [10][11][12], polylysine [13], chitosan [14,15], and gelatin [16], have also been reported as coating materials for biodegradable PLGA or poly (D,L-lactic acid) particles [17] as gene carriers. Cationic surface modification of nanoparticles can be obtained either during the formation of PLGA particles or by incubating polyelectrolytes with preformed PLGA particles [16], using emulsion-solvent evaporation or spray-drying techniques.…”

Section: Introductionmentioning

confidence: 99%

Understanding the adsorption mechanism of chitosan onto poly(lactide-co-glycolide) particles

Guo

Gemeinhart

2008

European Journal of Pharmaceutics and Biopharmaceutics

114

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Polyelectrolyte-coated nanoparticles or microparticles interact with bioactive molecules (peptides, proteins or nucleic acids) and have been proposed as delivery systems for these molecules. However, the mechanism of adsorption of polyelectrolyte onto particles remains unsolved. In this study, cationic poly(lactide-co-glycolide) (PLGA) nanoparticles were fabricated by adsorption of various concentrations of a biodegradable polysaccharide, chitosan (0-2.4 g/L), using oil-in-water emulsion and solvent evaporation techniques. The particle diameter, zeta-potential, and chitosan adsorption of chitosan coated PLGA nanoparticles confirmed the increase of polyelectrolyte adsorption. Five adsorption isotherm models (Langmuir, Freundlich, Halsey, Henderson and Smith) were applied to the experimental data in order to better understand the mechanism of adsorption. Both particle diameter and chitosan adsorption increased with chitosan concentration during adsorption. A good correlation was obtained between PLGA-chitosan nanoparticle size and adsorbed chitosan on the surface, suggesting the increased particle size was primarily due to the increased chitosan adsorption. The zeta-potential of chitosan-coated PLGA nanoparticles was positive and increased with chitosan adsorbed until a maximum value (+55 mV) was reached at approximately 0.4-0.6 g/L; PLGA nanoparticles had a negative zeta-potential (−20 mV) prior to chitosan adsorption. Chitosan adsorption on PLGA nanoparticles followed a multilayer adsorption behavior, although the Langmuir monolayer equation held at low concentrations of chitosan. The underlying reasons for adsorption of chitosan on PLGA nanoparticles were thought to be the cationic nature of chitosan, high surface energy and microporous non-uniform surface of PLGA nanoparticles.

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Polyethylenimine with acid-labile linkages as a biodegradable gene carrier

Cited by 313 publications

References 18 publications

Novel degradable oligoethylenimine acrylate ester-based pseudodendrimers for in vitro and in vivo gene transfer

Novel degradable oligoethylenimine acrylate ester-based pseudodendrimers for in vitro and in vivo gene transfer

Polyethylenimine-based nanocarriers in co-delivery of drug and gene: a developing horizon

Understanding the adsorption mechanism of chitosan onto poly(lactide-co-glycolide) particles

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