Polygala paniculata: um recurso de salicilato de metila produzido por cultura de tecidos vegetais

Abstract

RESUMOPolygala paniculata: um recurso de salicilato de metila produzido por cultura de tecidos vegetais O estabelecimento de cultura in vitro de Polygala paniculata L. permitiu a propagação rápida de matéria-prima vegetal sob condições assépticas e padronizadas. As plantas in vitro foram submetidas à extração e destilação simultâneas (EDS) e a composição química do óleo essencial foi analisada por CG/DIC e CG/EM. Foram avaliados os efeitos de BAP (6-benzilaminopurina) sobre o desenvolvimento in vitro e produção de voláteis em P. paniculata. A principal característica do óleo essencial consistiu na presença marcante de salicilato de metila. Plantas cultivadas em meio controle MS (Murashige & Skoog) produziram mais do que 80% de salicilato, enquanto o uso de BAP, reduziu a produção de salicilato de metila a 71% e 21% para as concentrações de 2 e 4 mg L -1 , respectivamente. A cultura de tecidos de P. paniculata pode ser indicada como fonte de salicilato de metila. The establishment of Polygala paniculata L. tissue culture allowed a rapid propagation of raw plant material under aseptic and standardized conditions. Plantlets were subjected to simultaneous distillation-extraction (SDE) and volatiles composition analyzed by GC/FID and GC/MS. The effect of BAP (6-benzylaminopurine) on in vitro development and volatiles production was evaluated. The main feature of volatile compounds was the significant amount of methyl salicylate. Plantlets cultured on control MS (Murashige & Skoog) medium showed more than 80% of salicylate, while the addition of BAP at 2 and 4 mg L -1 concentrations reduced methyl salicylate production to 71% and 21%, respectively. Tissue culture of P. paniculata proved to be a potential source of methyl salicylate. Palavras

Vehiculation of Methyl Salicylate from Microcapsules Supported on Textile Matrix

“…Figure 1 shows the spectrograms of the microcapsules that were obtained, as well as those of the wall materials, gelatin and gum Arabic and of the active ingredient methyl salicylate. Methyl salicylate has in its molecule intramolecular hydrogen bonds between hydroxyl and carbonyl groups [20]. The bands in the regions of 3175 cm −1 and 2952 cm −1 are attributed to the hydroxyl group and the asymmetrical stretching of the CH2, respectively.…”

“…The gum Arabic has in its chemical structure carboxylic groups, which justifies the presence of negative charges. The bands at 1603 cm −1 and 1421 cm −1 are derived from the asymmetric and symmetric stretching vibration of carboxylic acid (−COO ), respectively, which is attributed to the Methyl salicylate has in its molecule intramolecular hydrogen bonds between hydroxyl and carbonyl groups [20]. The bands in the regions of 3175 cm −1 and 2952 cm −1 are attributed to the hydroxyl group and the asymmetrical stretching of the CH 2 , respectively.…”

“…Generally, the treatment involves external application to the affected area of a drug that has methyl salicylate as its active ingredient. However, it has some drawbacks, such as low water solubility and dosage control, as well as high volatility [18][19][20]. This means that the concentration of methyl salicylate should be controlled to ensure effective delivery of the drug, in order to achieve anti-inflammatory and analgesic effects and, on the other hand, to ensure that there is no risk of skin irritation or burns [17,[20][21][22].…”

“…However, it has some drawbacks, such as low water solubility and dosage control, as well as high volatility [18][19][20]. This means that the concentration of methyl salicylate should be controlled to ensure effective delivery of the drug, in order to achieve anti-inflammatory and analgesic effects and, on the other hand, to ensure that there is no risk of skin irritation or burns [17,[20][21][22]. Yang et al [19] carried out a microencapsulation study in which they used chitosan as a wall material, having observed that the microencapsulation process provided a controlled release when compared to free methyl salicylate.…”

Determination of toxicity and chromatographic analysis of spilanthol content in in vitro culture of Spilanthes oleracea Jacq.

“…In addition, co-cultivation of nodal segments of S. oleracea and Polygala paniculata L. was performed. P. paniculata L. in vitro culture demonstrated production and release of methyl salicylate according to Victorio et al (2011). The cultures were maintained in a growth chamber under cool-white fluorescent lighting tubes (1.6 W m -2 , 23 µmol.m -2 s -1 and daily photoperiod of 16 h at 25 ± 2°C).…”