Polygalacturonase production by Aspergillus nomius MR103 in solid state fermentation using Agro-industrial wastes

Abstract

The present study was aimed at polygalacturonase production from Aspergillus nomius MR103 under solid state fermentation. A total of 57 fungal strains were obtained from mangrove soils collected from Gilakaladindi and Malakayalanka of Krishna District Andhra Pradesh. For the isolation of fungi these Soil samples were serially diluted and plated on pectin agar media plates. Among them, the isolate which showed maximum polygalacturonase activity was selected for this study. This strain was identified as A. nomius MR 103 by 18S rRNA sequences analysis. Pectin rich agro-industrial wastes like apple peel, citrus peel, orange peel, wheat bran, rice bran and sugarcane bagasse were used as substrates for polygalacturonase production by A. nomius MR 103. This strain was inoculated into the nutrient broth containing agro industrial wastes under solid state fermentation and amount of Polygalacturonase production was estimated. Maximum enzyme production of 4.83 IU/mg was recorded at pH 7.0 and temperature 35?C after 7 days of incubation, when orange peels were used as substrate. Addition of carbon and nitrogen sources to orange peel media improved the Polygalcturonase production. Sucrose as carbon and peptone as nitrogen sources were proved to be the best for maximum production of Polygalcturonase by A. nomius MR 103 on orange peel substrate. Utilization of agro-industrial by-products provided the establishment of a cost-efficient and sustainable process for enzyme production.

Industrially relevant pectinase production from Aspergillus parvisclerotigenus KX928754 using apple pomace as the promising substrate

“…Moreover, the generation of growth biomass showed a similar kind of trend with respect to pH which was maximum (0.114 ± 0.003 g/50 ml) at pH 7.0 (Figure 2a). The results are in concordance with the findings of Barkavi et al [45] and Ketipally et al [46] who have used A. niger and A. nomius, respectively. Various microbes are also known to produce pectinase that is active at higher pH and is termed alkaline pectinase.…”

“…In the present investigation, the organism was cultured with AP, where the culture has to produce the enzyme required for degrading the substrate, followed by utilization of the same for growth that explains the slow growth and production of pectinase which strongly support with findings of Barkavi et al [45], Abbasi et al [34] and Ketipally et al [46]. A few strains of Aspergillus are also known to produce pectinase in a short period of time [41,46,48]. The time required for fermentation depends upon certain factors such as the amount of nutrients in the medium, nature of the medium, physiological parameters and fermentative microorganisms [49,50].…”

“…Few organisms are known to produce pectinase within a short period of incubation time because of its fast-metabolic activity and/ or simple nutrients in the media, while some are also slow and that depend on the complex nutrients supplied (agro-residues). In the present investigation, the organism was cultured with AP, where the culture has to produce the enzyme required for degrading the substrate, followed by utilization of the same for growth that explains the slow growth and production of pectinase which strongly support with findings of Barkavi et al [45], Abbasi et al [34] and Ketipally et al [46]. A few strains of Aspergillus are also known to produce pectinase in a short period of time [41,46,48].…”

“…Various studies have shown that the addition of different carbon sources into media has increased the rate of enzyme production. The organisms were able to utilize the supplemented carbon sources and produce pectinase which is sensitive to implemented carbon sources [41,44,46,52,53]. The lowest pectinase was produced by using media supplemented with mannitol (1389.21 ± 25.83 U/ml), which was about 31% of less production in comparison to sucrose (1829.27 ± 14.01 U/ml).…”

An analysis of exo-polygalacturonase bioprocess in submerged and solid-state fermentation by Pleurotus ostreatus using pomelo peel powder as carbon source

“…Sucrose improved exo-polygalacturonase production in ssf system while a marked inhibition was observed in smf (Fig. 2), supported by Ketipally et al [18] who observed that addition of sucrose to orange peel media improved the polygalacturonase production in solid-state fermentation of Aspergillus nomius. Enzyme induction ratio of tannase [19] and xylanase [20] in the presence of sucrose was also reported to increase in solid-state fermentation.…”