Polygenic sex determination in the cichlid fish Astatotilapia burtoni

Roberts, Natalie B.; Juntti, Scott A.; Coyle, Kaitlin P.; Dumont, Bethany L.; Stanley, M. Kaitlyn; Ryan, Allyson Q.; Fernald, Russell D.; Roberts, Reade B.

doi:10.1186/s12864-016-3177-1

Cited by 71 publications

(84 citation statements)

References 92 publications

(132 reference statements)

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“…It has been suggested that teleost karyotypes have remained largely stable since the fish-specific whole genome duplication more than 300 million years ago [34]. This is in contrast to recent reports of chromosomal fusions among closely related cichlid species [35][36][37], and a large number of putative inversions associated with the evolution of sex chromosomes in various species [13,31,38]. Chromosomescale assemblies of cichlids allow us to quantify the levels of synteny among teleost lineages, and the rate of intra-chromosomal rearrangement among cichlid lineages in East Africa.…”

Section: Introductionmentioning

confidence: 71%

Chromosome-scale assemblies reveal the structural evolution of African cichlid genomes

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Joshi

Moore

et al. 2018

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Section: Introductionmentioning

confidence: 71%

Chromosome-scale assemblies reveal the structural evolution of African cichlid genomes

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Joshi

Moore

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“…Roberts et al. (2016), using a laboratory strain that is very likely from the same source population as the one from the University of Texas (HHL), also identified a XX/XY system on LG5 but an additional ZZ/ZW on LG13. Behavioral differences between the HH laboratory strain and southern populations (LZL and KA3) were observed in a study on maternal care (Renn et al., 2009).…”

Section: Discussionmentioning

confidence: 99%

“…In two recent studies dealing with the genomics of sex determination in A. burtoni, Böhne et al (2016) inferred a XX/XY system located on LG5 for the laboratory strain of the University of Basel (LAB), and a XX/XY system at LG18 for a wild population from the southern lineage (KAL). Roberts et al (2016), using a laboratory strain that is very likely from the same source population as the one from the University of Texas (HHL), also identified a XX/XY system on LG5 but an additional ZZ/ZW on LG13. Behavioral differences between the HH laboratory strain and southern populations (LZL and KA3) were observed in a study on maternal care (Renn et al, 2009).…”

Section: Interestingly In Both the Southern And The Northern Clades Ofmentioning

confidence: 98%

The puzzling phylogeography of the haplochromine cichlid fishAstatotilapia burtoni

Pauquet

Salzburger

Egger

2018

Ecology and Evolution

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Astatotilapia burtoni is a member of the “modern haplochromines,” the most species‐rich lineage within the family of cichlid fishes. Although the species has been in use as research model in various fields of research since almost seven decades, including developmental biology, neurobiology, genetics and genomics, and behavioral biology, little is known about its spatial distribution and phylogeography. Here, we examine the population structure and phylogeographic history of A. burtoni throughout its entire distribution range in the Lake Tanganyika basin. In addition, we include several A. burtoni laboratory strains to trace back their origin from wild populations. To this end, we reconstruct phylogenetic relationships based on sequences of the mitochondrial DNA (mtDNA) control region (d‐loop) as well as thousands of genomewide single nucleotide polymorphisms (SNPs) derived from restriction‐associated DNA sequencing. Our analyses reveal high population structure and deep divergence among several lineages, however, with discordant nuclear and mtDNA phylogenetic inferences. Whereas the SNP‐based phylogenetic hypothesis uncovers an unexpectedly deep split in A. burtoni, separating the populations in the southern part of the Lake Tanganyika basin from those in the northern part, analyses of the mtDNA control region suggest deep divergence between populations from the southwestern shoreline and populations from the northern and southeastern shorelines of Lake Tanganyika. This phylogeographic pattern and mitochondrial haplotype sharing between populations from the very North and the very South of Lake Tanganyika can only partly be explained by introgression linked to lake‐level fluctuations leading to past contact zones between otherwise isolated populations and large‐scale migration events.

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“…234 235 Sex determination in vertebrates is highly variable with the major exceptions of Eutherian 236 mammals and birds in which XX/XY and ZZ/ZW monofactorial sex determination systems 237 have been conserved over a long evolutionary period [45,46]. In contrast, fish exhibit much 238 more diverse and dynamic sex determination [9,10,47], with monofactorial and polyfactorial 239 [48,49] genetic systems and frequent switches and turnovers of master sex-determining genes 240 [12,14,15,17,21,50]. In goldfish, we identified male-specific markers and obvious male-241 specific SNPs strongly enriched on LG22.…”

Section: Discussion 188mentioning

confidence: 99%

Sex chromosome and sex locus characterization in the goldfish, Carassius auratus

Wen

Féron

Pan

et al. 2019

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38Background: Goldfish is an important model for various areas of research, including neural 39 development and behavior and a species of significant importance in aquaculture, especially as 40 an ornamental species. It has a male heterogametic (XX/XY) sex determination system that 41 relies on both genetic and environmental factors, with high temperatures being able to produce 42 female-to-male sex reversal. Little, however, is currently known on the molecular basis of 43 genetic sex determination in this important cyprinid model. We used sequencing approaches to 44 better characterize sex determination and sex-chromosomes in goldfish. 45 Results:Our results confirmed that sex determination in goldfish is a mix of environmental 46 and genetic factors and that its sex determination system is male heterogametic (XX/XY). 47Using reduced representation (RAD-seq) and whole genome (pool-seq) approaches, we 48 characterized sex-linked polymorphisms and developed male specific genetic markers. These 49 male specific markers were used to distinguish sex-reversed XX neomales from XY males and 50 to demonstrate that XX female-to-male sex reversal could even occur at a relatively low rearing 51 temperature (18°C), for which sex reversal has been previously shown to be close to zero. We 52 also characterized a relatively large non-recombining region (~11.7 Mb) on goldfish linkage 53 group 22 (LG22) that contained a high-density of male-biased genetic polymorphisms. This 54 large LG22 region harbors 373 genes, including a single candidate as a potential master sex 55 gene, i.e., the anti-Mullerian hormone gene (amh). However, no sex-linked polymorphisms 56 were detected in the goldfish amh gene or its 5 kb proximal promoter sequence. 57 Conclusions:These results show that goldfish have a relatively large sex locus on LG22, which 58 is likely the goldfish Y chromosome. The presence of a few XX males even at low temperature 59 also suggests that other environmental factors in addition to temperature could trigger female-60 to-male sex reversal. Finally, we also developed sex-linked genetic markers in goldfish, which 61 will be important for future research on sex determination and aquaculture applications in this 62 species. 63 assembly 65 66 3 BACKGROUND 67Goldfish, Carassius auratus, is a domesticated fish species originating from central Asia and 68 China that has been introduced throughout the world. Goldfish belongs to the Cyprinidae family 69 and is considered as an important fish model for research in endocrinology [1,2], 70 developmental biology [3,4] or fish pathology [5]. Thanks to the recent availability of a whole 71

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Polygenic sex determination in the cichlid fish Astatotilapia burtoni

Cited by 71 publications

References 92 publications

Chromosome-scale assemblies reveal the structural evolution of African cichlid genomes

Chromosome-scale assemblies reveal the structural evolution of African cichlid genomes

The puzzling phylogeography of the haplochromine cichlid fishAstatotilapia burtoni

Sex chromosome and sex locus characterization in the goldfish, Carassius auratus

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