Polyglycolide–montmorillonite as a novel nanocomposite platform for biosensing applications

Ünal, Betül Aydoğan; Yalçınkaya, Esra Evrim; Gümüştaş, Sıla; Sonmez, Burak; Özkan, Melek; Balcan, Mehmet; Demirkol, Dilek Odaci; Tımur, Suna

doi:10.1039/c7nj01751k

Cited by 17 publications

(4 citation statements)

References 52 publications

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“…Clays are inorganic materials, which are preferred to design biosensors because of their high adsorption capacity and stability [ 22 ]. Amino acids, calixarenes, and other numerous organic materials have been used to intercalate clays for their use in the design of biosensors [ 23 , 24 , 25 , 26 , 27 , 28 ]. Poly(amidoamine) (PAMAM) dendrimers with different generations were previously used to modify montmorillonite (Mt) clays [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Development of an Enzymatic Biosensor Using Glutamate Oxidase on Organic–Inorganic-Structured, Electrospun Nanofiber-Modified Electrodes for Monosodium Glutamate Detection

et al. 2023

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Herein, dendrimer-modified montmorillonite (Mt)-decorated poly-Ɛ-caprolactone (PCL) and chitosan (CHIT)-based nanofibers were prepared. Mt was modified with a poly(amidoamine) generation 1 (PAMAMG1) dendrimer, and the obtained PAMAMG1–Mt was incorporated into the PCL–CHIT nanofiber’s structure. The PCL–CHIT/PAMAMG1–Mt nanofibers were conjugated with glutamate oxidase (GluOx) to design a bio-based detection system for monosodium glutamate (MSG). PAMAMG1–Mt was added to the PCL–CHIT backbone to provide a multipoint binding side to immobilize GluOx via covalent bonds. After the characterization of PCL–CHIT/PAMAMG1–Mt/GluOx, it was calibrated for MSG. The linear ranges were determined from 0.025 to 0.25 mM MSG using PCL–CHIT/Mt/GluOx and from 0.0025 to 0.175 mM MSG using PCL–CHIT/PAMAMG1–Mt/GluOx (with a detection limit of 7.019 µM for PCL–CHIT/Mt/GluOx and 1.045 µM for PCL–CHIT/PAMAMG1–Mt/GluOx). Finally, PCL–CHIT/PAMAMG1–Mt/GluOx was applied to analyze MSG content in tomato soup without interfering with the sample matrix, giving a recovery percentage of 103.125%. Hence, the nanofiber modification with dendrimer-intercalated Mt and GluOx conjugation onto the formed nanocomposite structures was performed, and the PCL–CHIT/PAMAMG1–Mt/GluOx system was successfully developed for MSG detection.

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Section: Introductionmentioning

confidence: 99%

Development of an Enzymatic Biosensor Using Glutamate Oxidase on Organic–Inorganic-Structured, Electrospun Nanofiber-Modified Electrodes for Monosodium Glutamate Detection

et al. 2023

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“…Enzymatic glucose biosensors based on nanoclays have recently acquired attention due to their good performance in terms of detection limit, stability, sensibility, and selectivity for in vitro glucose determination from human biological fluids [57][58][59]. Several authors have applied raw and functionalized clays in biosensors' preparation for glucose detection, as stated in Table 5 [60][61][62][63][64][65][66][67][68][69][70][71][72]. A schematic representation of an enzyme biosensor based on electrode with nanoclay for glucose determination is shown in Figure 3 [23].…”

Section: Glucose Determinationmentioning

confidence: 99%

“…Under a similar strategy, Unal et al [68] prepared a polymer/clay nanocomposite based on natural silicate montmorillonite (Mt) and a biodegradable polymer polyglycolide (PGA). Then, the resulting matrix was used as a fixation matrix for pyranose oxidase (PyOx), which was selected as a model enzyme.…”

Section: Glucose Determinationmentioning

confidence: 99%

New Trends in Nanoclay-Modified Sensors

et al. 2021

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Nanoclays are widespread materials characterized by a layered structure in the nano-scale range. They have multiple applications in diverse scientific and industrial areas, mainly due to their swelling capacity, cation exchange capacity, and plasticity. Due to the cation exchange capacity, nanoclays can serve as host matrices for the stabilization of several molecules and, thus, they can be used as sensors by incorporating electroactive ions, biomolecules as enzymes, or fluorescence probes. In this review, the most recent applications as bioanalyte sensors are addressed, focusing on two main detection systems: electrochemical and optical methods. Particularly, the application of electrochemical sensors with clay-modified electrodes (CLME) for pesticide detection is described. Moreover, recent advances of both electrochemical and optical sensors based on nanoclays for diverse bioanalytes’ detection such as glucose, H2O2, organic acids, proteins, or bacteria are also discussed. As it can be seen from this review, nanoclays can become a key factor in sensors’ development, creating an emerging technology for the detection of bioanalytes, with application in both environmental and biomedical fields.

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“…The purpose of this review is to highlight the structural feature in the formation of clay minerals -their ability in forming peculiar layered atomic structures, each species within a group has specific properties. Consequently, clay minerals present a broad spectrum of applications in different areas: oil industry, paper industry, ceramic, agriculture, electronics, biomaterials pharmaceutics, cosmetics, environmental remediation, catalysts, polymer nanocomposites and other areas (CYGAN et al, 2009;HEINZ, 2012;JOSHI et al, 2009;KURIAN;KAVITHA, 2016;MAITI;BHATTACHARYYA, 2013;UNAL et al, 2017;ZHOU et al, 2016).…”

Section: Mixed Layered Clay Mineralsmentioning

confidence: 99%

Síntese De Nanocompósito Cerâmico De Zircônia-Hidroxiapatita

Lopes¹,

Flores²,

Prado³

et al. 2020

The Great World of Nanotechnology

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Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento, desde que sejam atribuídos créditos aos autores, e sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

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Polyglycolide–montmorillonite as a novel nanocomposite platform for biosensing applications

Abstract: In catalytic biosensors, the immobilization of biomolecules in a suitable matrix is one of the vital parameters for obtaining improved systems.

Cited by 17 publications

References 52 publications

Development of an Enzymatic Biosensor Using Glutamate Oxidase on Organic–Inorganic-Structured, Electrospun Nanofiber-Modified Electrodes for Monosodium Glutamate Detection

Development of an Enzymatic Biosensor Using Glutamate Oxidase on Organic–Inorganic-Structured, Electrospun Nanofiber-Modified Electrodes for Monosodium Glutamate Detection

New Trends in Nanoclay-Modified Sensors

Síntese De Nanocompósito Cerâmico De Zircônia-Hidroxiapatita

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