Polygoni Multiflori Radix Praeparata Ethanol Extract Exerts a Protective Effect Against High-Fat Diet Induced Non-Alcoholic Fatty Liver Disease in Mice by Remodeling Intestinal Microbial Structure and Maintaining Metabolic Homeostasis of Bile Acids

Abstract

In the prescription of Traditional Chinese Medicine for lipid metabolism, Polygoni Multiflori Radix Preparata (ZhiHeShouWu, RPMP) was widely used. In recent years, RPMP ethanol extract has been reported for the treatment of non-alcoholic fatty liver disease (NAFLD). However, the role of RPMP ethanol extract in the treatment of NAFLD has not been fully elucidated. Therefore, we examined the optimal therapeutic dose of RPMP ethanol extracts. Afterward, a mouse model of non-alcoholic fatty liver induced by a high-fat diet (HFD) was treated with RPMP ethanol extract to further evaluate the mechanism of action of RPMP ethanol extract treatment. And the serum lipid metabolism indexes and liver function indexes showed that the RPMP ethanol extract in the 1.35 g/kg dose group exhibited better therapeutic effects than the 2.70 g/kg dose group. Meanwhile, RPMP ethanol extract can regulate the biochemical indicators of serum and liver to normal levels, and effectively reduce liver steatosis and lipid deposition. RPMP ethanol extract treatment restored HFD-induced disruption of the compositional structure of the intestinal microbial (IM) and bile acids (BAs) pools. And restore the reduced expression of intestinal barrier-related genes caused by HFD administration, which also effectively regulates the expression of genes related to the metabolism of BAs in mice. Thus, RPMP ethanol extract can effectively improve the abnormal lipid metabolism and hepatic lipid accumulation caused by HFD, which may be related to the regulation of IM composition, maintenance of intestinal barrier function, and normal cholesterol metabolism in the body.

Dihydromyricetin ameliorated MCD mice by regulating the composition of serous lipids, bile acids and ileal microflora

“…As well known, 95% of BAs is reabsorbed into the portal vein at the end of the ileum and circulated to the liver, then absorbed into hepatocytes by Ntcp and Oatp1b2 [7]. The results indicated that the mRNA expression levels of hepatic Cyp7a1, Cyp27a1, Bsep, Mrp2, Ntcp and Oatp1b2 in MCD mice were signi cantly decreased as compared to MCS mice, which was is basically consistent with reported literatures [57,58] :3)] and harmful BAs (23-DCA and CA), and so it is for the regulatory trends of harmful bacteria (Erysipelotrichaceae and Faecalibaculum) and harmful lipids and BAs (Fig. 8).…”

Modified highland barley regulates lipid metabolism, liver inflammation and gut microbiota in high-fat/cholesterol diet mice as revealed by LC-MS based metabonomics

“…35 In our study, the PCoA results in HFCD-managed mice and HB-fed mice formed different clusters, FG was obviously separated from HBG, HB-1, HB-2 and HB-3, and HB resulted in closer clustering with NCG mice, indicating the ability of MHB to improve the structure of dysregulated gut microbiota. 36 In addition, MHB reversed the decrease of gut microbial diversity induced by the HFCD. At the gut microbial composition, MHB significantly decreased the abundances of Proteobacteria and Verrucomicrobia and increased the abundances of Firmicutes , Bacteroidetes , Actinobacteria and Deferribacteres .…”

Effects of plant natural products on metabolic-associated fatty liver disease and the underlying mechanisms: a narrative review with a focus on the modulation of the gut microbiota

“…in black bean juice and is often used clinically to regulate lipid metabolism. Studies have shown that supplementation with ZhiHeShouWu ethanol extract could increase the relative abundances of the SCFA-producing Phascolarctobacterium species and reduce the relative abundance of Desulfovibrio ( Dai et al., 2021 ). In addition, the ZhiHeShouWu ethanol extract affected BA metabolism by remodeling the gut microbiota, thus inhibiting the expression of gut FXR genes, accelerating cholesterol metabolism in vivo , and maintaining cholesterol homeostasis.…”