Polyherbal Medicine Divya Sarva-Kalp-Kwath Ameliorates Persistent Carbon Tetrachloride Induced Biochemical and Pathological Liver Impairments in Wistar Rats and in HepG2 Cells

Balkrishna, Acharya; Sakat, S; Ranjan, Ravikant; Joshi, Kheemraj; Shukla, Sunil; Joshi, Kamal; Verma, Sudeep; Gupta, Abhishek; Bhattacharya, Kunal; Varshney, Anurag

doi:10.3389/fphar.2020.00288

Cited by 9 publications

(19 citation statements)

References 59 publications

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“…SKK ameliorated the FFA-induced cell-death in the HepG2 cells. A similar protective response of SKK was earlier observed by us in HepG2 cells treated with carbon tetrachloride [ 19 ]. Plant metabolites comprising of rotenoids, catechin, gallic acid, quercetin, rutin, corilagin, and caffeic acid play a major role in the hepatoprotective role of SKK against a wide range of liver ailments [ 19 , 35 , 36 , 37 , 38 ].…”

Section: Discussionsupporting

confidence: 85%

“…Results showed the presence of polyphenols namely gallic acid, caffeic acid, quercetin, catechin, rutin, and corilagin. These plant metabolites have also been identified using liquid chromatography-based mass spectroscopy (LC–MS) and high-performance liquid chromatography (HPLC) techniques in our previously published study [ 19 ]. Chromatogram analysis showed all plant metabolites of SKK were well separated without any tail or diffuseness ( Figure 1 A–D).…”

Section: Resultsmentioning

confidence: 99%

“…In our previous study, showing the efficacy of SKK formulation in ameliorating carbon tetrachloride-induced liver damages, we had identified the presence of marker plant metabolites gallic acid, caffeic acid, quercetin, catechin, rutin, and corilagin originating from the plant components Boerhavia diffusa L., P. niruri sensu Hook. f., and Solanum nigrum L. [ 19 ]. Hence, in the present study, the same plant metabolic compounds were analyzed using the HPTLC as a quality check for SKK.…”

Section: Discussionmentioning

confidence: 99%

“…f. (Euphorbiaceae) (NISCAIR/RHMD/Consult/2019/3453-54-30), and Solanum nigrum L. (Solanaceae) (NISCAIR/RHMD/Consult/2019/3453-54-119) plants in the ratio of 2:1:1. B. diffusa L. plant also known as “Punarnava” possesses a variety of isoflavonoids such as rotenoids, flavonoids, flavonoid glycosides, xanthones, lignans, ecdysteroids, and steroids [ 19 ]. These plant metabolites have been found to have hepatoprotective effects.…”

Section: Introductionmentioning

confidence: 99%

“…In our previous study, we performed a detailed chemical analysis of SKK by High Performance Liquid Chromatography and Liquid Chromatography based–Mass Spectroscopy. We have reported the presence of plant metabolites: gallic acid, caffeic acid, rutin, quercetin, catechin, and corilagin as marker components originating from its herbal components [ 19 ]. We have shown that SKK exhibited hepatoprotective effect in Wistar rats and HepG2 cells against Carbon Tetrachloride (CCl 4 ) induced toxicity and inflammation [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Tri-Herbal Medicine Divya Sarva-Kalp-Kwath (Livogrit) Regulates Fatty Acid-Induced Steatosis in Human HepG2 Cells through Inhibition of Intracellular Triglycerides and Extracellular Glycerol Levels

et al. 2020

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Steatosis is characterized by excessive triglycerides accumulation in liver cells. Recently, application of herbal formulations has gained importance in treating complex diseases. Therefore, this study explores the efficacy of tri-herbal medicine Divya Sarva-Kalp-Kwath (SKK; brand name, Livogrit) in treating free fatty acid (FFA)-induced steatosis in human liver (HepG2) cells and rat primary hepatocytes. Previously, we demonstrated that cytosafe SKK ameliorated CCl4-induced hepatotoxicity. In this study, we evaluated the role of SKK in reducing FFA-induced cell-death, and steatosis in HepG2 through analysis of cell viability, intracellular lipid and triglyceride accumulation, extracellular free glycerol levels, and mRNA expression changes. Plant metabolic components fingerprinting in SKK was performed via High Performance Thin Layer Chromatography (HPTLC). Treatment with SKK significantly reduced the loss of cell viability induced by 2 mM-FFA in a dose-dependent manner. SKK also reduced intracellular lipid, triglyceride accumulation, secreted AST levels, and increased extracellular free glycerol presence in the FFA-exposed cells. SKK normalized the FFA-stimulated overexpression of SREBP1c, FAS, C/EBPα, and CPT1A genes associated with the induction of steatosis. In addition, treatment of rat primary hepatocytes with FFA and SKK concurrently, reduced intracellular lipid accumulation. Thus, SKK showed efficacy in reducing intracellular triglyceride accumulation and increasing extracellular glycerol release, along with downregulation of related key genetic factors for FFA-associated steatosis.

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Section: Discussionsupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tri-Herbal Medicine Divya Sarva-Kalp-Kwath (Livogrit) Regulates Fatty Acid-Induced Steatosis in Human HepG2 Cells through Inhibition of Intracellular Triglycerides and Extracellular Glycerol Levels

et al. 2020

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A systematic review on multipotent carcinogenic agent, N‐nitrosodiethylamine (NDEA), its major risk assessment, and precautions

Janmeda,

Jain,

Chaudhary

et al. 2024

J of Applied Toxicology

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The International Agency for Research on Cancer has classified N‐nitrosodiethylamine (NDEA) as a possible carcinogen and mutagenic substances, placing it in category 2A of compounds that are probably harmful to humans. It is found in nature and tobacco smoke, along with its precursors, and is also synthesized endogenously in the human body. The oral or parenteral administration of a minimal quantity of NDEA results in severe liver and kidney organ damage. The NDEA required bioactivation by CYP450 enzyme to form DNA adduct in the alkylation mechanism. Thus, this bioactivation directs oxidative stress and injury to cells due to the higher formation of reactive oxygen species and alters antioxidant system in tissues, whereas free radical scavengers guard the membranes from NDEA‐directed injury in many enzymes. This might be one of the reasons in the etiology of cancer that is not limited to a certain target organ but can affect various organs and organ systems. Although there are various possible approaches for the treatment of NDEA‐induced cancer, their therapeutic outcomes are still very dismal. However, several precautions were considered to be taken during handling or working with NDEA, as it considered being the best way to lower down the occurrence of NDEA‐directed cancers. The present review was designed to enlighten the general guidelines for working with NDEA, possible mechanism, to alter the antioxidant line to cause malignancy in different parts of animal body along with its protective agents. Thus, revelation to constant, unpredictable stress situations even in common life may remarkably augment the toxic potential through the rise in the oxidative stress and damage of DNA.

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Neuroprotection by Polyherbal Medicine Divya-Medha-Vati Against Scopolamine-Induced Cognitive Impairment Through Modulation of Oxidative Stress, Acetylcholine Activity, and Cell Signaling

Balkrishna,

Bhattacharya,

Shukla

et al. 2023

Mol Neurobiol

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Polyherbal Medicine Divya Sarva-Kalp-Kwath Ameliorates Persistent Carbon Tetrachloride Induced Biochemical and Pathological Liver Impairments in Wistar Rats and in HepG2 Cells

Cited by 9 publications

References 59 publications

Tri-Herbal Medicine Divya Sarva-Kalp-Kwath (Livogrit) Regulates Fatty Acid-Induced Steatosis in Human HepG2 Cells through Inhibition of Intracellular Triglycerides and Extracellular Glycerol Levels

Tri-Herbal Medicine Divya Sarva-Kalp-Kwath (Livogrit) Regulates Fatty Acid-Induced Steatosis in Human HepG2 Cells through Inhibition of Intracellular Triglycerides and Extracellular Glycerol Levels

A systematic review on multipotent carcinogenic agent, N‐nitrosodiethylamine (NDEA), its major risk assessment, and precautions

Neuroprotection by Polyherbal Medicine Divya-Medha-Vati Against Scopolamine-Induced Cognitive Impairment Through Modulation of Oxidative Stress, Acetylcholine Activity, and Cell Signaling

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