Polyhexamethylene guanidine hydrochloride modified sodium alginate nonwoven with potent antibacterial and hemostatic properties for infected full-thickness wound healing

Zhang, Jie; Hu, Lifen; Zhang, Qiang; Guo, Chuan; Wu, Chen‐Yi; Shi, Yao; Shu, Rui; Tan, Lin

doi:10.1016/j.ijbiomac.2022.04.194

Cited by 22 publications

(10 citation statements)

References 36 publications

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“…Investigation of literature data shows that the cytotoxic of PHMG depends on its concentration and mode of application in dressing matrix. Chemical grafting of PHMG to the dressing backbone is a promising method to mitigate this concern 18,19,54,55 …”

Section: Resultsmentioning

confidence: 99%

“…Chemical grafting of PHMG to the dressing backbone is a promising method to mitigate this concern. 18,19,54,55 The cytocompatibility of the prepared dressing membranes was investigated by evaluating the behavior of cultured L-929 fibroblast cells. Based on the observations made in Figure 5A-C it can be noted that there were no significant morphological changes in the cells cultured over P/G-PHMG1 and P/G-PHMG2 membranes.…”

Section: Biological Properties Of Dressing Membranementioning

confidence: 99%

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Polyhexamethylene guanidine hydrochloride‐functionalized wound dressings: Physical, mechanical and biological evaluations

Babaahmadi

Yeganeh

2023

Polymers for Advanced Techs

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The objective of the present study is fabrication of wound dressing membranes with effective antibacterial activity, good biocompatibility with fibroblast cells, appropriate mechanical properties in both dry and wet states to potentially provide physical protection to wounds at different stages of healing, and the ability to maintain an optimum moist environment over the wounded area. To achieve this, a new silane functional polyhexamethylene guanidine hydrochloride was synthesized and utilized as a crosslinker for a polymeric matrix consisting of poly(vinyl alcohol) and gelatin. Thin monolayer films of the resulting polymeric networks were characterized and subjected to different physical and biological assays. The tensile test results indicated that the prepared dressing membranes exhibit good tensile strength in both dry (44 MPa) and hydrated (700 KPa) states. The fluid handling capacity of the dressings was found to be in the range of 2.23 to 2.29 g 10−1 m−2 day−1, which is appropriate for treating wounds with “light” to “moderate” exudate levels. The MTT assay showed excellent biocompatibility, evidenced by high fibroblast cells viability cultured directly over dressings (over 70%) or cultured in media containing the leachates extracted from the dressings (over 90%). Moreover, the dressings exhibited potent antibacterial activity against Staphylococcus aureus (87% killing) and Escherichia coli (99% killing) bacterial strains.

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Section: Resultsmentioning

confidence: 99%

Section: Biological Properties Of Dressing Membranementioning

confidence: 99%

Polyhexamethylene guanidine hydrochloride‐functionalized wound dressings: Physical, mechanical and biological evaluations

Babaahmadi

Yeganeh

2023

Polymers for Advanced Techs

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“…Notably, the sterilization rate of cotton fabric-SD-PHMG 10 against E. coli and S. aureus could still maintain more than 99.9% even after 10 antibacterial cycles. As a large number of bacteria would contact with the fabrics during the antibacterial experiment, thus bacterial debris inevitably and gradually accumulated on the surface of testing fabrics accompanied by repeated antibacterial cycles, thus leading to the decline of antibacterial ability ( Zhang et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

“…This confirmed that the platelets on the surface of the cotton-SA-PHMG were activated, which was attributed to the effect of PHMG. The guanidine groups of PHMG are electropositive which have interactions with electronegative cell membranes on the red blood, leading to charming hemagglutination and adsorption of fibrinogen and plasma proteins, as well as accelerate the aggregation of the platelets ( Gharibi et al, 2019 ; Zhang J. et al, 2021 ; Zhang et al, 2022 ). Additionally, the carboxyl groups on SA came into being the complex with the Fe 3+ ions which were escaped from the damaged RBCs, resulting in the aggregation and adhesion of platelets ( Sekhon and Sen Gupta, 2018 ; Zheng et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

A universal coating strategy for inhibiting the growth of bacteria on materials surfaces

Zhang

Wang

et al. 2022

Front. Chem.

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The development of a versatile antibacterial coating, irrespective of material characteristics, is greatly attractive but still a challenge. In this work, mussel-inspired dopamine-modified sodium alginate (SA-DA) was successfully synthesized as the adhesion layer, and antibacterial coatings on three types of substrates, namely cotton fabric, aluminum sheet, and polyurethane membrane, were constructed through the layer-by-layer (LbL) deposition of polyhexamethylene guanidine and sodium alginate. Among the coated materials, the coated cotton fabric was systematically characterized, and the results showed that it still exhibited ideal hydrophilicity, and its liquid absorption capacity increased with an increase in the coating layers. The growth of Escherichia coli and Staphylococcus aureus was notably inhibited on the coated cotton fabric, and 10 coating bilayers achieved 100% inhibition of bacterial growth within 10 min. Furthermore, an ideal antibacterial ability maintained after 10 cycles of antibacterial trials or 50 washing or soaping cycles. In vitro evaluation of the hemostatic effect indicated that the coated cotton fabric could promote blood clotting by concentrating the components of blood and activating the platelets, and no significant hemolysis and cytotoxicity were observed in the coated cotton fabric. Moreover, the coated aluminum and polyurethane film also displayed an obvious antibacterial effect, which proved that the constructed coating could successfully adhere to the metal and polymer surfaces. Therefore, this work provided a proper way for the progress of a current antibacterial coating tactics for different substrate surfaces.

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“…Compounds containing the guanidine group constitute a class of bioactive molecules with a wide range of applications [ 10 , 29 ]. Thus, guanidine has been developed as the dominant structural motif in the design of novel drugs for the treatment of various infectious diseases [ 30 , 31 ]. IBG is a substituted benzoguanidine derivative with good antibacterial activity against Gram-positive and Gram-negative bacteria [ 13 , 14 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolite Identification of Isopropoxy Benzene Guanidine in Rat Liver Microsomes by Using UHPLC-Q-TOF-MS/MS

Zhang

et al. 2023

IJMS

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Isopropoxy benzene guanidine (IBG) is a guanidine derivative with antibacterial activity against multidrug-resistant bacteria. A few studies have revealed the metabolism of IBG in animals. The aim of the current study was to identify potential metabolic pathways and metabolites of IBG. The detection and characterization of metabolites were performed with high-performance liquid chromatography tandem mass spectrometry (UHPLC-Q-TOF-MS/MS). Seven metabolites were identified from the microsomal incubated samples by using the UHPLC-Q-TOF-MS/MS system. The metabolic pathways of IBG in the rat liver microsomes involved O-dealkylation, oxygenation, cyclization, and hydrolysis. Hydroxylation was the main metabolic pathway of IBG in the liver microsomes. This research investigated the in vitro metabolism of IBG to provide a basis for the further pharmacology and toxicology of this compound.

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Polyhexamethylene guanidine hydrochloride modified sodium alginate nonwoven with potent antibacterial and hemostatic properties for infected full-thickness wound healing

Cited by 22 publications

References 36 publications

Polyhexamethylene guanidine hydrochloride‐functionalized wound dressings: Physical, mechanical and biological evaluations

Polyhexamethylene guanidine hydrochloride‐functionalized wound dressings: Physical, mechanical and biological evaluations

A universal coating strategy for inhibiting the growth of bacteria on materials surfaces

Metabolite Identification of Isopropoxy Benzene Guanidine in Rat Liver Microsomes by Using UHPLC-Q-TOF-MS/MS

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