Polyhydroxyalkanoate as a slow-release carbon source for in situ bioremediation of contaminated aquifers: From laboratory investigation to pilot-scale testing in the field

Pierro, Lucia; Matturro, Bruna; Rossetti, Simona; Sagliaschi, Marco; Sucato, Salvatore; Alesi, Eduard J.; Bartsch, Ernst; Arjmand, Firoozeh; Papini, Marco Petrangeli

doi:10.1016/j.nbt.2016.11.004

Cited by 51 publications

(39 citation statements)

References 20 publications

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“…As an example, groundwater wells such as those based on the Groundwater Circulation Well ® technology (IEG, Germany), which are specifically designed to create a circulation flow of groundwater within the aquifer with the scope to mobilize contaminants and/or accelerate (anaerobic) biodegradation processes, are already commercially available (Pierro et al ., 2017). In these systems, the contaminated groundwater is recirculated several times within the well before it flows downstream, hence allowing to control the groundwater retention time within the treatment zone independently of the groundwater flow velocity.…”

Section: Resultsmentioning

confidence: 99%

The bioelectric well: a novel approach for in situ treatment of hydrocarbon‐contaminated groundwater

Palma

Daghio

Franzetti

et al. 2017

Microbial Biotechnology

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SummaryGroundwater contamination by petroleum hydrocarbons (PHs) is a widespread problem which poses serious environmental and health concerns. Recently, microbial electrochemical technologies (MET) have attracted considerable attention for remediation applications, having the potential to overcome some of the limiting factors of conventional in situ bioremediation systems. So far, field‐scale application of MET has been largely hindered by the limited availability of scalable system configurations. Here, we describe the ‘bioelectric well’ a bioelectrochemical reactor configuration, which can be installed directly within groundwater wells and can be applied for in situ treatment of organic contaminants, such as PHs. A laboratory‐scale prototype of the bioelectric well has been set up and operated in continuous‐flow regime with phenol as the model contaminant. The best performance was obtained when the system was inoculated with refinery sludge and the anode potentiostatically controlled at +0.2 V versus SHE. Under this condition, the influent phenol (25 mg l−1) was nearly completely (99.5 ± 0.4%) removed, with an average degradation rate of 59 ± 3 mg l−1 d and a coulombic efficiency of 104 ± 4%. Microbial community analysis revealed a remarkable enrichment of Geobacter species on the surface of the graphite anode, clearly pointing to a direct involvement of this electro‐active bacterium in the current‐generating and phenol‐oxidizing process.

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Section: Resultsmentioning

confidence: 99%

The bioelectric well: a novel approach for in situ treatment of hydrocarbon‐contaminated groundwater

Palma

Daghio

Franzetti

et al. 2017

Microbial Biotechnology

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“…In the second phase, starting from the hydrogeochemical conceptual model obtained from the first one, the analysis focused on the pilot test area, increasing the observation scale to analyze in detail the effects of geological heterogeneity and chemical peculiarity in the first intervention area. Based on the results of the laboratory investigations (microbiological and microcosm tests), the design and execution of a field test was then performed as the final step of the evaluation process [11,12,15]. The pilot test was designed to optimize the layout of the intervention, to check its efficiency, and to calibrate the full-scale intervention.…”

Section: Methodsmentioning

confidence: 99%

“…The combined technology is verified through a pilot test (the first of its kind) carried out in an operative industrial site located in the Po plain, which site is heavily contaminated by CAHs. Based on the satisfactory results of the laboratory investigations (microbiological and microcosm tests), which indicated that reductive dechlorination might be successfully enhanced at the considered site [12,[15][16][17], the design and execution of a field test was performed as the final step of the evaluation process [11,12,15]. The pilot test aimed to verify the possible mobilization of contaminants trapped in horizons with very low permeability, and the effective distribution of electron donors to stimulate the in situ processes of natural attenuation [15].…”

Section: The Combination Of Groundwater Circulation Wells and Biologimentioning

confidence: 99%

“…The analytical data, which refer to the monitoring campaigns carried out from 2008 to 2017, were used for the construction of some contour maps, which represent the concentrations of the contaminants detected in the historical piezometric network. The thematic maps were produced by considering some key contaminants (vinyl chloride (VC) and 1,2-dicloroethylene (1,2-DCE)), which are correlated with the state of contamination that was previously ascertained [12,15,45]. The concentration values were compared to the Italian threshold limits (CSC)-the limits between each class corresponded to multiples of the CSC value for each contaminant.…”

Section: Contamination Status Evolutionmentioning

confidence: 99%

“…The high-resolution characterization, in geological and chemical terms, displays the association of a significant mass of contaminants with lenses of fine materials. These very low permeability layers, in the saturated zone, act as slow-release sources of contaminants [12,15]. 1,2-DCE and VC were the main pollutants of the groundwater.…”

Section: Contamination Status Evolutionmentioning

confidence: 99%

See 2 more Smart Citations

Hydrogeochemical Model Supporting the Remediation Strategy of a Highly Contaminated Industrial Site

Ciampi

Esposito

Papini

2019

Water

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Delineation and understanding the geology and the hydrogeology of a contaminated site, considering its chemical and its biological aspects, are fundamental requirements for successful environmental remediation. The aim of this research is to provide some evidence about the effectiveness of a hydrogeochemical geodatabase to facilitate the integrated management, representation and analysis of heterogeneous data, enabling the appropriate selection, design and optimization of an effective remediation strategy. This study investigates a new technology for the remediation of a dense non-aqueous phase liquid aged source zone, with the aim of enhancing in situ bioremediation by coupling groundwater circulation wells with a continuous production system of electron donors. The technology was verified through a pilot test carried out at an industrial site highly contaminated by chlorinated aliphatic hydrocarbons. The multidisciplinary conceptual model confirmed a complex hydrogeological situation, with the occurrence of active residual sources in low permeability layers. The pilot test results clearly demonstrate a significant mobilization of contaminants from the low permeability zone, and the possibility of favoring the in situ natural attenuation mechanisms based upon biological reductive dechlorination. Different information related to the hydrogeochemical sphere must be integrated and taken into consideration when developing a reliable remediation strategy for contaminated sites.

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Mapping biosynthesis pathway of natural folate during Lactiplantibacillus plantarum subsp. plantarum fermentation in purple cabbage by transcriptomic analysis

Tan,

Yan,

Ramaswamy

et al. 2024

J Sci Food Agric

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BACKGROUNDFolate is an important one‐carbon cycle donor involved in the synthesis of purines, thymine, pantothenic acid, serine and glycine. The present study aimed to explore the capacity of Lactiplantibacillus plantarum subsp. plantarum (L. plantarum subsp. plantarum) to synthesize folate in fermenting MRS and purple cabbage juice (PCJ). The mechanism underlying folate biosynthesis was investigated by transcriptomics. We aimed to find important genes and transcription factors involved in folate production by analyzing gene expression during L. plantarum subsp. plantarum fermentation.RESULTSThe results showed a 44% increase in total folate content in the PCJ medium, with 5‐methyltetrahydrofolate (5‐MTHF) contributing to a substantial 28% of this increase, whereas no folate was detected during MRS fermentation. The mechanism of folate production during the fermentation involved the coordinated regulation of multiple genes. The expression of some key genes (aroC, pabA, folE, folA and MTHFR) was upregulated during fermentation, and the expression of MTHFR, a gene related to 5‐MTHF synthesis, was increased by 8.4 times. These genes play roles in different steps of the folate synthesis pathway, including substrate synthesis, metabolic regulation and catalytic reactions.CONCLUSIONOur results revealed the expression of all genes involved in the biosynthesis of 5‐MTHF during L. plantarum subsp. plantarum fermentation, indicating the integrity of its biosynthetic pathway. The findings provide a scientific basis for folate production by optimizing fermentation conditions, further helping research on folate synthesis in other fermented foods. © 2024 Society of Chemical Industry.

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Polyhydroxyalkanoate as a slow-release carbon source for in situ bioremediation of contaminated aquifers: From laboratory investigation to pilot-scale testing in the field

Cited by 51 publications

References 20 publications

The bioelectric well: a novel approach for in situ treatment of hydrocarbon‐contaminated groundwater

The bioelectric well: a novel approach for in situ treatment of hydrocarbon‐contaminated groundwater

Hydrogeochemical Model Supporting the Remediation Strategy of a Highly Contaminated Industrial Site

Mapping biosynthesis pathway of natural folate during Lactiplantibacillus plantarum subsp. plantarum fermentation in purple cabbage by transcriptomic analysis

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