Polyhydroxyalkanoate/Carbon Nanotube Nanocomposites: Flexible Electrically Conducting Elastomers for Neural Applications

Vallejo-Giraldo, Catalina; Pugliese, Eugenia; Larrañaga, Aitor; Fernández‐Yagüe, Marc A.; Britton, James; Trotier, Alexandre; Tadayyon, Ghazal; Kelly, Adriona; Rago, Ilaria; Sarasua, Jose‐Ramon; Dowd, Eilís; Quinlan, Leo R.; Pandit, Abhay; Biggs, Manus

doi:10.2217/nnm-2016-0075

Cited by 38 publications

(47 citation statements)

References 83 publications

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“…In order to determine the stability of PEDOT:PTS functionalized microelectrodes, substrates were subjected to 500 cycles of continuous potentiodynamic stimulation with the aim of testing, under working conditions, the durability and the electrochemical robustness of the potential neural electrodes . The loss in electroactivity of the microelectrodes was calculated as a percentage based on CSCs recorded before and after stability studies (Table ).…”

Section: Resultsmentioning

confidence: 99%

“…The electrochemical stability of microelectrodes was determined as described previously in ref. . Briefly, cyclic voltammetry was performed before and after electrical stability measurements and used to calculate the percentage of loss in charge storage capacity by comparing the CSC of the 1st and 500th cycles.…”

Section: Methodsmentioning

confidence: 99%

“…Biological Characterization—Immunofluorescent Labeling : Indirect double‐immunofluorescent labeling was performed to visualize neurons and astrocyte cell populations, as described by Vallejo‐Giraldo et al Briefly, VM cells on experimental and control substrates were fixed with 4% paraformaldehyde and 1% of sucrose for 20 min at room temperature at the time point. Once fixed, the samples were washed with PBS and permeabilized with buffered 0.5% Triton X‐100 within a buffered isotonic solution (10.3 g sucrose, 0.292 g NaCl, 0.06 g MgCl 2 , 0.476 g 4‐(2‐hydroxyethyl)‐1 piperazineethanesulfonic acid (HEPES) buffer, 0.5 mL Triton X‐100, in 100 mL water, pH 7.2) at 4 °C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

“…Cell analysis was performed as described in ref. . At least twenty images at 60× magnification were taken at random from each experimental group and controls.…”

Section: Methodsmentioning

confidence: 99%

“…Implantable stimulation and recording devices have received significant attention in biomedical engineering and have brought great success in the treatment of central nervous system disorders including paralysis, epilepsy, and Parkinson's disease . In order to achieve chronic functionality and integration with surrounding tissue, material functionalization strategies have been employed to provide chemical and physicomechanical properties analogous to neural tissues, with an ultimate goal of mitigating electrode deterioration via reactive host cell response and glial scar‐induced encapsulation …”

Section: Introductionmentioning

confidence: 99%

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Attenuated Glial Reactivity on Topographically Functionalized Poly(3,4‐Ethylenedioxythiophene):P‐Toluene Sulfonate (PEDOT:PTS) Neuroelectrodes Fabricated by Microimprint Lithography

Vallejo-Giraldo

Krukiewicz

Calaresu

et al. 2018

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Following implantation, neuroelectrode functionality is susceptible to deterioration via reactive host cell response and glial scar-induced encapsulation. Within the neuroengineering community, there is a consensus that the induction of selective adhesion and regulated cellular interaction at the tissue-electrode interface can significantly enhance device interfacing and functionality in vivo. In particular, topographical modification holds promise for the development of functionalized neural interfaces to mediate initial cell adhesion and the subsequent evolution of gliosis, minimizing the onset of a proinflammatory glial phenotype, to provide long-term stability. Herein, a low-temperature microimprint-lithography technique for the development of micro-topographically functionalized neuroelectrode interfaces in electrodeposited poly(3,4-ethylenedioxythiophene):p-toluene sulfonate (PEDOT:PTS) is described and assessed in vitro. Platinum (Pt) microelectrodes are subjected to electrodeposition of a PEDOT:PTS microcoating, which is subsequently topographically functionalized with an ordered array of micropits, inducing a significant reduction in electrode electrical impedance and an increase in charge storage capacity. Furthermore, topographically functionalized electrodes reduce the adhesion of reactive astrocytes in vitro, evident from morphological changes in cell area, focal adhesion formation, and the synthesis of proinflammatory cytokines and chemokine factors. This study contributes to the understanding of gliosis in complex primary mixed cell cultures, and describes the role of micro-topographically modified neural interfaces in the development of stable microelectrode interfaces.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Cell analysis was performed as described in ref. . At least twenty images at 60× magnification were taken at random from each experimental group and controls.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Attenuated Glial Reactivity on Topographically Functionalized Poly(3,4‐Ethylenedioxythiophene):P‐Toluene Sulfonate (PEDOT:PTS) Neuroelectrodes Fabricated by Microimprint Lithography

Vallejo-Giraldo

Krukiewicz

Calaresu

et al. 2018

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Carbon Nanotubes, Directly Grown on Supporting Surfaces, Improve Neuronal Activity in Hippocampal Neuronal Networks

et al. 2019

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Carbon nanotube (CNT)-modified surfaces unequivocally demonstrate their biocompatibility and ability to boost the electrical activity of neuronal cells cultured on them. Reasons for this effect are still under debate. However, the intimate contact at the membrane level between these thready nanostructures and cells, in combination with their unique electrical properties, seems to play an important role. The entire existing literature exploiting the effect of CNTs on modulating cellular behavior deals with cell cultures grown on purified multiwalled carbon nanotubes (MWNTs) deposited on a supporting surface via drop-casting or mechanical entrapment. Here, for the first time, it is demonstrated that CNTs directly grown on a supporting silicon surface by a chemical vapor deposition (CVD)-assisted technique have the same effect. It is shown that primary neuronal cells developed above a carpet of CVD CNTs form a healthy and functional network. The resulting neuronal network shows increased electrical activity when compared to a similar network developed on a control glass surface. The low cost and high versatility of the here presented CVD-based synthesis process, together with the possibility to create on supporting substrate patterns of any arbitrary shape of CNTs, open up new opportunities for brain-machine interfaces or neuroprosthetic devices.

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Preparation of Cytocompatible ITO Neuroelectrodes with Enhanced Electrochemical Characteristics Using a Facile Anodic Oxidation Process

Vallejo-Giraldo

Pampaloni

Pallipurath

et al. 2017

Adv Funct Materials

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Physicochemical modification of implantable electrode systems is recognized as a viable strategy to enhance tissue/electrode integration and electrode performance in situ. In this work, a bench-top electrochemical process to formulate anodized ITO films with altered roughness, thickness and conducting profiles was explored. In addition, the influence of these anodized films on SH-5YSY cell proliferation, viability and focal adhesion reinforcement indicated that anodized ITO film cytocompatibility can be altered by varying the anodization current density. Furthermore, an ITO anodized films formed with a current density of 0.4 mA cm-2 showed important primary neural cell survival and promotion of neural network activity.

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Polyhydroxyalkanoate/Carbon Nanotube Nanocomposites: Flexible Electrically Conducting Elastomers for Neural Applications

Abstract: This study demonstrates the feasibility of a green thermoplastic MWCNTs nanocomposite for potential use in neural interfacing applications.

Cited by 38 publications

References 83 publications

Attenuated Glial Reactivity on Topographically Functionalized Poly(3,4‐Ethylenedioxythiophene):P‐Toluene Sulfonate (PEDOT:PTS) Neuroelectrodes Fabricated by Microimprint Lithography

Attenuated Glial Reactivity on Topographically Functionalized Poly(3,4‐Ethylenedioxythiophene):P‐Toluene Sulfonate (PEDOT:PTS) Neuroelectrodes Fabricated by Microimprint Lithography

Carbon Nanotubes, Directly Grown on Supporting Surfaces, Improve Neuronal Activity in Hippocampal Neuronal Networks

Preparation of Cytocompatible ITO Neuroelectrodes with Enhanced Electrochemical Characteristics Using a Facile Anodic Oxidation Process

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