Polyhydroxyalkanoate inclusion-body growth and proliferation in<i>Bacillus megaterium</i>

McCool, Gabriel J.; Fernández, Tania Miñes; Li, Ning; Cannon, Maura C.

doi:10.1111/j.1574-6968.1996.tb08132.x

Cited by 48 publications

(12 citation statements)

References 23 publications

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“…One exception is the study of McCool and co-workers on the structure of PHB granules from Bacillus megaterium. 27 Transmission electron microscopy images of PHB granules isolated from cells after different periods of cultivation revealed thin bridges between granules. However, the influence of phasins was not investigated and discussed in this study.…”

Section: Resultsmentioning

confidence: 99%

Studies on the Influence of Phasins on Accumulation and Degradation of PHB and Nanostructure of PHB Granules inRalstoniaeutrophaH16

et al. 2006

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Phasins play an important role in the formation of poly(3-hydroxybutyrate) [PHB] granules and affect their size and number in the cells. Recent studies on the PHB granule proteome and analysis of the complete genomic DNA sequence of Ralstonia eutropha H16 have identified three homologues of the phasin protein PhaP1. In this study, mutants of R. eutropha deficient in the expression of the phasin genes phaP1, phaP2, phaP3, phaP4, phaP12, phaP123, and phaP1234 were examined by gas chromatography. In addition, the nanostructures of the PHB granules of the wild-type and of the mutants were imaged by atomic force microscopy (AFM), and the molecular masses of the accumulated PHB were analyzed by gel permeation chromatography. For this, cells were cultivated under conditions permissive for accumulation of PHB and were then cultivated under conditions permissive for degradation of PHB. Mutants deficient in the expression of phaP2, phaP3, or phaP4 genes mobilized the stored PHB only slowly like the wild-type, whereas degradation occurred much earlier and faster in the phaP1 single mutant as well as in all multiple mutants defective in the phaP1 gene plus one or more other phasin genes. This indicated that the presence of the major phasin PhaP1 on the granule surface is important for PHB degradation and that this phasin is therefore of particular relevance for PHB accumulation. It was also shown that the molecular weights of the accumulated PHB were identical in all examined strains; phasins have therefore no influence on the molecular weight of PHB. The AFM images obtained in this study showed that the PHB granules of R. eutropha H16 form a single interconnected system inside the wild-type cells.

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Section: Resultsmentioning

confidence: 99%

Studies on the Influence of Phasins on Accumulation and Degradation of PHB and Nanostructure of PHB Granules inRalstoniaeutrophaH16

et al. 2006

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“…Few studies have documented the accumulation of PHB in Bacillus sp. during the late log phase or early stationary phase; however, the accumulated PHB was found to be degraded at the onset of sporulation (Chen et al 1991; McCool et al 1996). An optimized medium might enhance and maintain PHA content even at the instance of sporulation in Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Polyhydroxybutyrate production in Bacillus mycoides DFC1 using response surface optimization for physico-chemical process parameters

Narayanan

Ramana

2012

3 Biotech

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The production of polyhydroxybutyrate (PHB) by Bacillus sp. is most often growth associated and is influenced by various physico-chemical parameters. Imbalanced nutrient conditions were often found to result in sporulation and low PHB production in Bacillus sp. In the present investigation, Bacillus mycoides DFC1 strain isolated from garden soil was studied for PHB production in glucose–peptone broth. The effect of glucose/peptone ratio on biomass yield, PHB production and sporulation was investigated. Central composite rotatable design was used to study the interactive effects of three variables: glucose, peptone and pH on cell growth and PHB production. The optimized medium conditions with the constraint ‘to maximize’ cell growth and PHB content were glucose 17.34 g/l, peptone 7.03 g/l at pH 7.3. A maximum dry cell weight of 4.35 g/l and PHB yield of 3.32 g/l amounting to 76.32 % (w/w) of dry cell weight with negligible sporulation at the end of 72 h resulted in a significant increase (1.83–3.32 g/l or 1.82-fold) in the production of PHB in comparison to the medium used in preliminary studies.

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“…Hydrogenated inclusions were obtained by the growth of P. oleovorans in M4 medium [4] containing sufficient nitrogen to increase biomass, followed by a PHO production phase under nitrogen limitation. Deuterated inclusions were obtained via a two-phase fermentation.…”

Section: Production and Isolation Of Pha Inclusions Bodiesmentioning

confidence: 99%

Investigation of the phase morphology of bacterial PHA inclusion bodies by contrast variation SANS

Russell

Holden

Garvey

et al. 2006

Physica B: Condensed Matter

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Polyhydroxyalkanoate inclusion-body growth and proliferation inBacillus megaterium

Cited by 48 publications

References 23 publications

Studies on the Influence of Phasins on Accumulation and Degradation of PHB and Nanostructure of PHB Granules inRalstoniaeutrophaH16

Studies on the Influence of Phasins on Accumulation and Degradation of PHB and Nanostructure of PHB Granules inRalstoniaeutrophaH16

Polyhydroxybutyrate production in Bacillus mycoides DFC1 using response surface optimization for physico-chemical process parameters

Investigation of the phase morphology of bacterial PHA inclusion bodies by contrast variation SANS

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